Fungos filamentosos isolados de sedimento do manguezal barra das Jangadas, Jaboatão dos Guararapes, Pernambuco, Brasil

Abstract

Os manguezais são ecossistemas de transição entre os ambientes terrestre e marinho caracterizados por propriedades físico-químicas únicas, influenciando a biota local. Tendo em vista a instabilidade ecológica do manguezal Barra das Jangadas, provocado pelos deságues dos rios Jaboatão e Pirapama, torna-se importante o conhecimento de espécies de fungos filamentosos desse ecossistema. Este trabalho teve como objetivo isolar e identificar fungos filamentosos de sedimentos do manguezal Barra das Jangadas, Jaboatão dos Guararapes, Pernambuco. Foram coletadas amostras de sedimento do manguezal Barra das Jangadas nos meses de março e abril/2004 e outubro/2005 (período de estiagem); junho e julho/2004 e julho/2005 (período chuvoso). As coletas foram realizadas em quatro pontos: 1 e 3, 2 e 4, ao longo dos rios Jaboatão e Pirapama, respectivamente. Vinte e quatro amostras foram coletadas e semeadas em placas de Petri contendo ágar Sabouraud acrescido de cloranfenicol. Foram isoladas e identificadas 50 espécies de fungos filamentosos, perfazendo um total de 273 UFC. Penicillium e Aspergillus foram os gêneros dominantes com 21 e 11 espécies respectivamente, seguidos de Trichoderma (5), Fusarium, Phoma e Talaromyces (2), sendo os demais gêneros: Cladosporium, Eupenicillium, Gongronella, Microsphaeropsis, Mucor, Stilbella e Thielavia com uma espécie. Dentre as espécies isoladas Gongronella butleri e Stilbella clavispora são consideradas como primeira ocorrência em sedimento de manguezal para o Brasil. Palavras-chave: Fungos filamentosos, manguezais, taxonomia

Density and diversity of filamentous fungi in the water and sediment of Araçá bay in São Sebastião, São Paulo, Brazil

“…Similar findings were reported by Gomes et al (2011), who analyzed filamentous fungi isolated from sediment collected from a mangrove swamp in Barra das Jangadas, Pernambuco, Brazil. The authors reported a higher incidence of fungi during the dry season (77%) relative to the rainy season (23%) and found that the species Aspergillus terreus, A. niger, Penicillium sp., and Trichoderma sp.…”

“…The authors reported a higher incidence of fungi during the dry season (77%) relative to the rainy season (23%) and found that the species Aspergillus terreus, A. niger, Penicillium sp., and Trichoderma sp. were common in both seasons (Gomes et al 2011). Pinto et al (1992) also found that rainfall significantly increases the amount of isolated fungi in both land and aquatic ecosystems.…”

<b>Tolerance to Polycyclic Aromatic Hydrocarbons (PAHs) by filamentous fungi isolated from contaminated sediment in the Amazon region

“…All locations were characterized by having different levels of PAHs contamination, as described by Souza et al (2015) (Table 1). Fungal isolation was performed by serial suspension technique, based on Gomes, Cavalcanti, and Passavante (2011) in which 25 g of sediment samples were diluted in 225 mL of sterile distilled water and further dilutions (1:1000) were performed seeding 1 mL in petri plates containing Sabouraud Dextrose Agar (SDA; 40 g L ) in quadruplicate. The inoculated plates were incubated at 28°C for 5 days and purified isolates were preserved in vials containing sterile distilled water and cryopreservation tubes at -20°C.…”

Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation

“…It is also necessary to take into consideration the special conditions of the Negro River sediments regarding their granulometry, as mentioned by Souza et al (2015), and the small amounts of substrates for fungal colonization. Gomes et al (2011) and Da Silva et al (2003) reported that the diversity of substrates and nutrient availability are abiotic factors that determine the presence, permanence and survival of mycobiota. It is important to mention that of the 21 isolates selected for their oxidation capacity of gallic acid, nine isolates (S03, S10, S15, S29, S50, S55, S123, S125, S143) belong to the regions of Porto São Raimundo and Ceasa, regions characterized by Souza et al (2015) to be highly contaminated.…”

“…All locations were characterized by having different levels of PAHs contamination, as described by Souza et al (2015) (Table 1). Fungal isolation was performed by serial suspension technique, based on Gomes et al (2011) in which 25 g of sediment samples were diluted in 225 mL of sterile distilled water and further dilutions (1:1000) were performed seeding 1 mL in petri plates containing Sabouraud Dextrose Agar (SDA; 40 g L −1 dextrose, 10 g L −1 mycological peptone, and 15 g L −1 agar) supplemented with chloramphenicol (100 mg L −1 ) in quadruplicate. The inoculated plates were incubated at 28°C for 5 days and purified isolates were preserved in vials containing sterile distilled water (Castellani 1939) and cryopreservation tubes at −20°C.…”