The use of AMF can contribute to plant nutrition, reduce the use of chemical fertilizers and help with the ecological balance of agricultural crops. The research aimed to investigate the diversity of AMF in the soils of the Chapada das Mesas National Park (PNCM) and select species capable of promoting the growth of faba beans. The experiment was conducted in a greenhouse, with six treatments and five replications. The treatments included soil without AMF inoculum (control), soil with Glomus spp., soil with Rhizophagus sp., soil from all areas collected at PNCM, soil with Roottela BR® and soil with corrected phosphorus concentration. The diversity assessment found that the pasture area presented a greater diversity of AMF species. The genus Glomus showed greater predominance in both phytophysiognomies. It was not possible to find a significant impact of AMF inoculation on the growth and productivity of faba beans. The study highlights the importance of further research to determine the time required for the establishment of AMF in faba bean roots and to evaluate the effectiveness of AMF inoculation on plant productivity in different crops and substrates, in addition to providing important data for studies of conservationists in the region.