Fungus Polygalacturonase-Generated Oligogalacturonide Restrains Fruit Softening in Ripening Tomato

Yang, Ying; Lu, Laifeng; Sun, Dandan; Wang, Jinghao; Wang, Nifei; Liao, Qiao; Guo, Qingbin; Wang, Changlu

doi:10.1021/acs.jafc.1c04972

Cited by 8 publications

(3 citation statements)

References 38 publications

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“…65 Glucose metabolism plays a role in cell division, dilation and degradation, and contributes to fruit softening during ripening. 20,32,66 Endogenous glucanase, 67 PG, 68,69 expansion protein, 70,71 pectin methylesterase 72 and pectate lyase 73 have been reported to play important roles in cell degradation and fruit softening. In this study, ABA treatment induced the upregulation of most proteins associated with cell wall metabolism (especially PG).…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic analysis of tomato fruit ripening behavior in response to exogenous abscisic acid

Cui

et al. 2023

J Sci Food Agric

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BACKGROUNDTo determine how abscisic acid (ABA) affects tomato fruit ripening at the protein level, mature green cherry tomato fruit were treated with ABA, nordihydroguaiaretic acid (NDGA) or sterile water (control, CK). The proteomes of treated fruit were analyzed and quantified using tandem mass tags (TMTs) at 7 days after treatment, and the gene transcription abundances of differently expressed proteins (DEPs) were validated with quantitative real‐time polymerase chain reaction.RESULTSPostharvest tomato fruit underwent faster color transformation and ripening than the CK when treated with ABA. In total, 6310 proteins were identified among the CK and treatment groups, of which 5359 were quantified. Using a change threshold of 1.2 or 0.83 times, 1081 DEPs were identified. Among them, 127 were upregulated and 127 were downregulated in the ABA versus CK comparison group. According to KEGG and protein–protein interaction network analyses, the ABA‐regulated DEPs were primarily concentrated in the photosynthesis system and sugar metabolism pathways, and 102 DEPs associated with phytohormones biosynthesis and signal transduction, pigment synthesis and metabolism, cell wall metabolism, photosynthesis, redox reactions, allergens and defense responses were identified in the ABA versus CK and NDGA versus CK comparison groups.CONCLUSIONABA affects tomato fruit ripening at the protein level to some extent. The results of this study provided comprehensive insights and data for further research on the regulatory mechanism of ABA in tomato fruit ripening. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Quantitative proteomic analysis of tomato fruit ripening behavior in response to exogenous abscisic acid

Cui

et al. 2023

J Sci Food Agric

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“…Fruit firmness is one of the important characteristics of tomato fruit ripening, and the metabolism of the cell wall plays a crucial role in determining the change rate of fruit firmness during ripening [ 67 ]. Several enzymes, including endogenous glucanase [ 68 ], polygalacturonase (PG) [ 69 , 70 ], expansin [ 71 ], pectin methylesterase [ 72 ], and PL [ 73 ], were shown to be involved in cell wall metabolism and fruit softening [ 74 ]. In addition, Zeng et al [ 67 ] found that ABA treatment can accelerate fruit softening and promote the gene expression of β-galactosidase, SlTBG3 , and SlTBG4 .…”

Section: Discussionmentioning

confidence: 99%

ChIP-Seq Analysis of SlAREB1 Downstream Regulatory Network during Tomato Ripening

Cui

et al. 2023

Foods

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SlAREB1, a member of the abscisic acid (ABA) response element-binding factors (AREB/ABFs) family, was reported to play a crucial role in the expression of ABA-regulated downstream genes and affect the ripening of tomato fruit. However, the downstream genes of SlAREB1 are still unclear. Chromatin immunoprecipitation (ChIP) is a powerful tool and a standard method for studying the interactions between DNA and proteins at the genome-wide level. In the present study, SlAREB1 was proved to continually increase until the mature green stage and then decrease during the ripening period, and a total of 972 gene peaks were identified downstream of SlAREB1 by ChIP-seq analysis, mainly located in the intergenic and promoter regions. Further gene ontology (GO) annotation analysis revealed that the target sequence of SlAREB1 was the most involved in biological function. Kyoto Encylopaedia of Genes and Genomes (KEGG) pathway analysis showed that the identified genes were mainly involved in the oxidative phosphorylation and photosynthesis pathways, and some of them were associated with tomato phytohormone synthesis, the cell wall, pigment, and the antioxidant characteristic of the fruit as well. Based on these results, an initial model of SlAREB1 regulation on tomato fruit ripening was constructed, which provided a theoretical basis for further exploring the effects of the regulation mechanism of SlAREB1 and ABA on tomato fruit ripening.

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“…Moreover, ectopic expression of FaPE1 promoted the content of aromatic amino acids phenylalanine, tryptophan, tyrosine, and aspartate. More recently, Yang et al [ 85 ] reported that OGs prepared from apple pectin through enzymatic digestion using a mixture of PG and PL from Botrytis cinerea inhibited fruit softening in ripening tomatoes. This research suggest that OGs are indeed capable of serving as signals to regulate plant growth and development.…”

Section: Cwi Signal Candidates In Fruit Ripeningmentioning

confidence: 99%

Cell Wall Integrity Signaling in Fruit Ripening

Jia

Wang

Zhang

et al. 2023

IJMS

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Plant cell walls are essential structures for plant growth and development as well as plant adaptation to environmental stresses. Thus, plants have evolved signaling mechanisms to monitor the changes in the cell wall structure, triggering compensatory changes to sustain cell wall integrity (CWI). CWI signaling can be initiated in response to environmental and developmental signals. However, while environmental stress-associated CWI signaling has been extensively studied and reviewed, less attention has been paid to CWI signaling in relation to plant growth and development under normal conditions. Fleshy fruit development and ripening is a unique process in which dramatic alternations occur in cell wall architecture. Emerging evidence suggests that CWI signaling plays a pivotal role in fruit ripening. In this review, we summarize and discuss the CWI signaling in relation to fruit ripening, which will include cell wall fragment signaling, calcium signaling, and NO signaling, as well as Receptor-Like Protein Kinase (RLKs) signaling with an emphasis on the signaling of FERONIA and THESEUS, two members of RLKs that may act as potential CWI sensors in the modulation of hormonal signal origination and transduction in fruit development and ripening.

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Fungus Polygalacturonase-Generated Oligogalacturonide Restrains Fruit Softening in Ripening Tomato

Cited by 8 publications

References 38 publications

Quantitative proteomic analysis of tomato fruit ripening behavior in response to exogenous abscisic acid

Quantitative proteomic analysis of tomato fruit ripening behavior in response to exogenous abscisic acid

ChIP-Seq Analysis of SlAREB1 Downstream Regulatory Network during Tomato Ripening

Cell Wall Integrity Signaling in Fruit Ripening

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