Anaerobic
digestion of furfural wastewater suffers from toxicity
to microbes, which reduces its performance. The toxic effects may
be eliminated by a sustainable way of conductive material supplementation.
In this study, the effect on anaerobic digestion of furfural (0.5–4
g/L) was investigated by adding 0–40 g/L granular activated
carbon (GAC) in batch tests. The innovation of this current study
is to explore the potential direct interspecies electron transfer
(DIET) for the first time in furfural degradation as the sole substrate
in anaerobic digestion. The results demonstrated that when furfural
was the sole substrate, in the presence of GAC, the peak methane production
rate was increased by 46.0%, while the specific methane yield was
improved by 24.7%, compared with the control. Additionally, the electroactive Geobacter sulfurreducens abundance in biofilms attached
to the surface of GAC was 19.0 folds higher than that in the control.
The abundances of the related genes of methane metabolism and furfural
degradation in biofilms were 1.1 and 5.8 folds higher than the control
by metagenomic analysis, respectively. These lines of evidence suggested
that the pathway of furfural metabolism to methane among Moorella species, G. sulfurreducens, and Methanothrix species based on
DIET was likely established.