Further Characterization of Cells Expressing STRO-1 in Cultures of Adult Human Bone Marrow Stromal Cells

Stewart, Karina; Walsh, S.; Screen, Joanne; Jefferiss, Carolyn M.; Chainey, Jonathan; Jordan, Grant; Beresford, J.N.

doi:10.1359/jbmr.1999.14.8.1345

Cited by 184 publications

(142 citation statements)

References 62 publications

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“…More surprising than the osteogenic phenotype of cultured HPDCs in this study was the presence of a consistent and stable Stro-1 + population. Stro-1 expression had been reported as varying between 2% and 80% in 28-day bone marrow stromal cultures [39] and noted to decrease, to 15% of their maximum frequency, after 6 weeks of culture [36]. These phenotypic expression profile differences noted in our study may reflect differences in culture techniques and cell types examined (eg, stromal versus periosteal cell isolates).…”

Section: Discussionmentioning

confidence: 53%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering. Questions/purpose We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro. Methods We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1 À and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription-polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization. Results Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture.

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Section: Discussionmentioning

confidence: 53%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…RNA samples that had not been reversetranscribed were included to check for the absence of DNA contamination. Primers sequences for PTH/PTHrpreceptor (PTH/PTHrpR) (Stewart et al, 1999), osteonectin (ON) (Gronthos et al, 1999), dentin sialophosphoprotein (DSPP) (Papagerakis et al, 2002) and glyceraldehyde phosphate dehydrogenase (GAPDH) (Bluteau et al, 1999) and annealing temperatures are detailed in table 1. Amplifications were carried out in an Eppendorf master cycler (VWR, Brumath, France) under the following conditions: denaturation for 3 minutes at …”

Section: Rt-pcrmentioning

confidence: 99%

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Lopez-Cazaux¹,

Bluteau²,

Magne³

et al. 2006

eCM

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In vitro approaches have extensively been developed to study reparative dentinogenesis. While dental pulp is a source of unidentified progenitors able to differentiate into odontoblast-like cells, we investigated the effect of two media; MEM (1.8mM Ca and 1mM Pi) and RPMI 1640 (0.8mM Ca and 5mM Pi) on the behaviour of human dental pulp cells. Our data indicate that MEM significantly increased cell proliferation and markedly enhanced the proportion of α-smooth muscle actin positive cells, which represent a putative source of progenitors able to give rise to odontoblast-like cells. In addition, MEM strongly stimulated alkaline phosphatase activity and was found to induce expression of transcripts encoding dentin sialophosphoprotein, an odontoblastic marker, without affecting that of parathyroid hormone/parathyroid hormone related protein-receptor and osteonectin. In conclusion, these observations demonstrate that not only proliferation but also differentiation into odontoblast-like cells was induced by rich calcium and poor phosphate medium (MEM) as compared to RPMI 1640. This study provides important data for the determination of the optimal culture conditions allowing odontoblast-like differentiation in human pulp cell culture.

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“…Well-known exceptions include STRO1 [10,11], THY1 [12,13], and SCA1 [14]. Determining the genetic expression profile of this specific cell type is key to rapid advances in understanding skeletal growth and development.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression Profile of Human Bone Marrow Stromal Cells: High-Throughput Expressed Sequence Tag Sequencing Analysis

et al. 2002

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Human bone marrow stromal cells (HBMSC) are pluripotent cells with the potential to differentiate into osteoblasts, chondrocytes, myelosupportive stroma, and marrow adipocytes. We used high-throughput DNA sequencing analysis to generate 4258 single-pass sequencing reactions (known as expressed sequence tags, or ESTs) obtained from the 5Ј (97) and 3Ј (4161) ends of human cDNA clones from a HBMSC cDNA library. Our goal was to obtain tag sequences from the maximum number of possible genes and to deposit them in the publicly accessible database for ESTs (dbEST of the National Center for Biotechnology Information). Comparisons of our EST sequencing data with nonredundant human mRNA and protein databases showed that the ESTs represent 1860 gene clusters. The EST sequencing data analysis showed 60 novel genes found only in this cDNA library after BLAST analysis against 3.0 million ESTs in NCBI's dbEST database. The BLAST search also showed the identified ESTs that have close homology to known genes, which suggests that these may be newly recognized members of known gene families. The gene expression profile of this cell type is revealed by analyzing both the frequency with which a message is encountered and the functional categorization of expressed sequences. Comparing an EST sequence with the human genomic sequence database enables assignment of an EST to a specific chromosomal region (a process called digital gene localization) and often enables immediate partial determination of intron/exon boundaries within the genomic structure. It is expected that high-throughput EST sequencing and data mining analysis will greatly promote our understanding of gene expression in these cells and of growth and development of the skeleton.Key Words: human bone marrow stromal cells, EST sequencing analysis, novel genes, gene expression profile, data mining them potentially useful for cell and gene therapy [2][3][4]. After extensive proliferation in vitro, the HBMSC population includes precursor cells for at least four types of connective tissue: bone, cartilage, hematopoiesis-supporting stroma, and associated adipocytes [5][6][7][8]. When single bone marrow stromal cells develop into individual HBMSC colonies, they show different morphologies and rates of proliferation. HBMSC strains derived from individual colonies also vary widely in their ability to form bone and the hematopoietic microenvironment after in vivo transplantation [9]. Despite efforts to understand the biology of HBMSC through studies 8Article doi:10.1006/geno.2001.6683, available online at http://www.idealibrary.com on IDEAL at different levels, the study of genes and proteins important to the biological phenotypes of HBMSC is still in its infancy. Well-known exceptions include STRO1 [10,11], THY1 [12,13], and SCA1 [14]. Determining the genetic expression profile of this specific cell type is key to rapid advances in understanding skeletal growth and development. The ability to peer into HBMSC and read their molecular signature will enable us to identify more preci...

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Further Characterization of Cells Expressing STRO-1 in Cultures of Adult Human Bone Marrow Stromal Cells

Cited by 184 publications

References 62 publications

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

Gene Expression Profile of Human Bone Marrow Stromal Cells: High-Throughput Expressed Sequence Tag Sequencing Analysis

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