Further characterization of mitochondrial contact sites: Effect of short-chain alcohols on membrane fluidity and activity

Ardail, Dominique; Lermé, F.; Louisot, Pierre

doi:10.1016/s0006-291x(05)80868-x

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…At high catecholamine concentrations, when all NMG have disappeared, zones of close contact between inner and outer membrane are frequently seen. The presence of cardiolipin in the matrix granules, as suggested by different authors (Barnard and Afzelius, 1972;Bonucci et al, 1973;Brdiczka et al, 1980), and their integration within the inner membrane could explain why the cardiolipin content is 30% greater in the contact sites than in the inner membrane (Ardail et al, 1990). The presence of glycoprotein in the matrix granules (Bonucci et al, 1973;Taffarel et al, 1984) may reduce the Ca concentration, necessary for the fusion of the inner and outer membranes, from the millimolar to the micromolar range (Dahl et al, 1979;Gratzl et al, 1980;Papahadjopoulos et al, 1991), as is the situation in heart muscle.…”

Section: Contact Sitesmentioning

confidence: 83%

Mitochondrial matrix granules: Their behavior during changing metabolic situations and their relationship to contact sites between inner and outer mitochondrial membranes

Jacob

Hertsens

Biermans

1994

Microscopy Res & Technique

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Since their discovery in the early fifties mitochondrial granules have been the subject of many researches. Some twenty years ago two hypotheses on their function were introduced. Peachey thought that the granules were a sink of cations and that they would eventually regulate the concentrations of these ions. Alternatively, Barnard thought that the granules were precursors of the mitochondrial inner membrane. There are only a few data on organic constituents of the granules. Phospholipids (e.g., cardiolipin) glycoprotein or lipids, calcium precipitable lipoprotein, cytochrome c oxidase seem to be present in the granules. There has been much debate on whether calcium is present or not. Reports are mostly based on X-ray microanalysis, the result of which depends on preparation techniques. In heart muscle in stimulating situations the NMG (native matrix granules) move towards the inner membrane and are incorporated in it. They appear to create contact sites between inner and outer mitochondrial membranes in which enzymes can function efficiently. It is hypothetized that the system, NMG-contact sites, forms the structural basis of a regulatory mechanism, by which cells can cope with a high and sudden energy demand.

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Section: Contact Sitesmentioning

confidence: 83%

Mitochondrial matrix granules: Their behavior during changing metabolic situations and their relationship to contact sites between inner and outer mitochondrial membranes

Jacob

Hertsens

Biermans

1994

Microscopy Res & Technique

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“…Evidence suggests that cardiolipin plays a key role in regulating mitochondrial bioenergetics, by optimizing activities of various mitochondrial inner membrane proteins involved in OXPHOS [121-123]. Cardiolipin has a role in maintaining contact sites between the inner and outer mitochondrial membranes [124]. Cardiolipin has also been noted to be required for electron transfer in the mitochondrial respiratory chain [125].…”

Section: Mechanisms Of Mitochondrial Dysfunction In Diabetic Cardimentioning

confidence: 99%

Mitochondrial dysfunction in diabetic cardiomyopathy

Duncan

2011

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

173

122

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Cardiovascular disease is common in patients with diabetes and is a significant contributor to the high mortality rates associated with diabetes. Heart failure is common in diabetic patients, even in the absence of coronary artery disease or hypertension, an entity known as diabetic cardiomyopathy. Evidence indicates that myocardial metabolism is altered in diabetes, which likely contributes to contractile dysfunction and ventricular failure. The mitochondria are the center of metabolism, and recent data suggests that mitochondrial dysfunction may play a critical role in the pathogenesis of diabetic cardiomyopathy. This review summarizes many of the potential mechanisms that lead to mitochondrial dysfunction in the diabetic heart.

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“…diate density fractions (IDF) isolated from organelles of various sources, certain outer and inner membrane proteins are enriched (e.g., Ohlendieck et al, 1986;Pon et al, 1989;Lithgow et al, 1991). In addition, the lipid composition quantitatively differs from purified outer and inner membrane vesicles (Ardail et al, 1990a). These quantitative differences might, however, in part be the consequence of enrichment of the inner boundary membrane in the IDF, whereas the inner membrane fractions mainly contain cristae membranes.…”

Section: Contact Sitesmentioning

confidence: 99%

A morphological view on mitochondrial protein targeting

Klei

Veenhuis

Neupert

1994

Microscopy Res & Technique

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Mitochondrial protein targeting includes both intramitochondrial sorting of proteins encoded by the organellar genome and import and subsequent sorting of nuclear encoded precursor proteins. Only a few proteins are encoded by the mitochondrial genome and synthesized in the organellar matrix. These include predominantly inner membrane proteins that are perhaps co-translationally inserted into this membrane. Biochemical data suggest that insertion into the inner membrane may be confined to the inner boundary membrane. Ultrastructurally, however, a preferential association of ribosomes with either inner boundary or cristae membranes has not been established.The majority of the mitochondrial proteins are nuclear encoded and synthesized as precursors in the cytosol. Electron microscopic studies revealed that import of precursor proteins is generally confined to sites where both mitochondrial envelope membranes are closely apposed. In line with these observations, biochemical studies indicated that precursor proteins destined for the inner membrane or matrix have t o interact with the energized inner membrane to allow complete passage of the precursor through the outer membrane. As a consequence, the mitochondrial envelope membranes have to be in close proximity at protein import sites.In isolated mitochondria distinct sites (designated as contact sites) exist where both envelope membranes are closely apposed and presumably stably associated. In situ, however, mitochondrial boundary membranes are in close proximity over large areas that cover almost the entire mitochondrial periphery. Consequently, the relative area of the mitochondrial surface, where both boundary membranes are in sufficient proximity for allowing protein translocation, is generally larger in situ compared to that in isolated organelles.Immunocytochemical localization studies showed a rather random distribution of components of the mitochondrial protein translocation machinery over the entire mitochondrial surface and not confined to contact sites.Based on these ultrastructral data and recent biochemical findings we propose that mitochondrial protein import sites are dynamic in nature and include relatively labile regions of close association of the boundary membranes. In vitro, however, mitochondrial protein import may preferentially take place at or near the presumably stable contact sites. o

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Further characterization of mitochondrial contact sites: Effect of short-chain alcohols on membrane fluidity and activity

Cited by 18 publications

References 23 publications

Mitochondrial matrix granules: Their behavior during changing metabolic situations and their relationship to contact sites between inner and outer mitochondrial membranes

Mitochondrial matrix granules: Their behavior during changing metabolic situations and their relationship to contact sites between inner and outer mitochondrial membranes

Mitochondrial dysfunction in diabetic cardiomyopathy

A morphological view on mitochondrial protein targeting

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