Tests of over 2000 pigs, with the blood group reagents Ac, Ap and 0, and the reagents Ha, Hb, Hc, Hd, He and Hc/c, confirmed that the H locus affects the expression of A and 0 antigens. The 'exceptional' phenotypes Ap and '-' werz found in Ha homozygotes of Miniature pigs and in Hcd homozygotes of a semiinbred Landrace line. They were also found in unrelated pigs of other breeds, and moreover in some individuals with genotypes H a H c d , HabHCJ, H-H-and HaHab or H a b H a b .The mechanism of the effect of the H locus on the A phenotypes may consist in the interaction of certain, not yet completely defined, H genotypes, or in linkage of the H system with the hypothetical 'S' locus.The aim of the present study was to investigate which alleles (H genotypes) influence, or are otherwise associated with, the expression of A and 0 antigens.