Further evidence for the DNA base specificity of light-induced banding

Ferruchhi, L.; Sumner, A. T.

doi:10.1007/bf01041352

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…The possible photodenaturation of DNA was suggested previously by others (25,26,27). It has been suggested that denaturation of DNA might occur as a result of photosensitized oxidation of guanine in fixed cells and isolated DNA (27,28,29). However, these authors did not demonstrate that the light-induced changes in chromatographic DNA mobility (27) or staining pattern of mitotic chromosomes (25,26) that they observed were due to a transition between ds and ss DNA.…”

Section: Discussionmentioning

confidence: 83%

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei¶

Bernaś¹,

Asem²,

Robinson³

et al. 2005

Photochem Photobiol

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The double-stranded helical structure of DNA is maintained in part by hydrogen bonds between strands and by stacking interactions between adjacent purine and pyrimidine bases in one strand. The transition (denaturation) from a double-stranded (ds) to a single-stranded (ss) form can be induced in isolated DNA or fixed cells by exposure to elevated temperatures, alkali or acids, aprotic or nonpolar solvents or some drugs. We report here that DNA denaturation can occur in situ in cell nuclei as a result of interaction between light and an intercalated dye, acridine orange or ethidium bromide. This DNA photodenaturation was probed using metachromatic properties of acridine orange and imaged by fluorescence confocal microscopy. Furthermore, an empirical kinetic model was developed to separate changes of acridine orange luminescence intensities caused by photobleaching from those that were a result of DNA denaturation. We investigated the influence of oxygen on these phenomena and propose a mechanism by which photodenaturation may occur.

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Section: Discussionmentioning

confidence: 83%

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei¶

Bernaś¹,

Asem²,

Robinson³

et al. 2005

Photochem Photobiol

View full text Add to dashboard Cite

show abstract

“…The possible photodenaturation of DNA was suggested previously by others (25,26,27). It has been suggested that denaturation of DNA might occur as a result of photosensitized oxidation of guanine in fixed cells and isolated DNA (27,28,29). However, these authors did not demonstrate that the light‐induced changes in chromatographic DNA mobility (27) or staining pattern of mitotic chromosomes (25,26) that they observed were due to a transition between ds and ss DNA.…”

Section: Discussionmentioning

confidence: 99%

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei^¶

Bernaś¹,

Asem²,

Robinson³

et al. 2005

Photochem & Photobiology

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The double‐stranded helical structure of DNA is maintained in part by hydrogen bonds between strands and by stacking interactions between adjacent purine and pyrimidine bases in one strand. The transition (denaturation) from a double‐stranded (ds) to a single‐stranded (ss) form can be induced in isolated DNA or fixed cells by exposure to elevated temperatures, alkali or acids, aprotic or nonpolar solvents or some drugs. We report here that DNA denaturation can occur in situ in cell nuclei as a result of interaction between light and an intercalated dye, acridine orange or ethidium bromide. This DNA photodenaturation was probed using metachromatic properties of acridine orange and imaged by fluorescence confocal microscopy. Furthermore, an empirical kinetic model was developed to separate changes of acridine orange luminescence intensities caused by photobleaching from those that were a result of DNA denaturation. We investigated the influence of oxygen on these phenomena and propose a mechanism by which photodenaturation may occur.

show abstract

Further evidence for the DNA base specificity of light-induced banding

Cited by 2 publications

References 12 publications

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei¶

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei¶

Confocal Fluorescence Imaging of Photosensitized DNA Denaturation in Cell Nuclei^¶

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