Further evidence from tryptic peptide analysis for heterogeneity among type D retroviruses

Abstract

Tryptic peptide analyses were performed on the major internal structural proteins of type D retroviruses isolated originally from subhuman primate species (MPMV, SMRV, LV) and from permanent human cell lines (HeLaV, HEp-2V, PMFV). The p25 peptide maps of MPMV, LV, PMFV and HEp-2V were very similar but showed a striking dissimilarity to the p25 map of HeLaV and the p35 map of SMRV. All type D viruses included in this study could be distinguished by peptide maps of at least two of their three low-molecular-weight proteins p10, p12 and p15. These studies further demonstrate the heterogeneity among type D retroviruses.

Biochemical and immunological characterization of structural proteins from retrovirus-D/New England and comparison to Mason-Pfizer monkey virus and permanent human fibroblast virus

“…Previously we were able to use the technique of tryptic peptide analysis to distinguish between the low-molecular-weight proteins of different type D retroviruses (26). To further characterize the proteins of R-D/NE we compared tryptic peptide maps of p 4, p 10/p 12, p 15, and p 27 to those of MPMV and PMFV.…”

“…Viral structural proteins were sliced from the gel and tryptic peptide analysis of ~2~I labelled proteins were performed on cellulose thin layer chromatography plates (10 × 10 cm) (Merck, Federal Republic of Germany) as described previously (26).…”

“…These viruses resemble MPMV in many respects but can be clearly distinguished by their different glycoprotein pattern (28) and tryptic peptide maps of tow-molecular-weight proteins (26). lgecently, type D retroviruses have been isolated from several macaque species at several Primate Centers in the U.S. (5,15,16,23).…”