Further evidence that culture media affect perinatal outcome: findings after transfer of fresh and cryopreserved embryos

Nelissen, E. C. M.; Montfoort, Aafke P.A. van; Coonen, Edith; Derhaag, Josien G.; Geraedts, J. P. M.; Smits, Luc; Land, Jolande A.; Evers, Johannes L.H.; Dumoulin, J.C.M.

doi:10.1093/humrep/des145

Cited by 182 publications

(138 citation statements)

References 55 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…Indeed, there is some evidence to suggest that the culture environment may affect embryo development, fetal growth, birthweight, and perinatal outcome [47][48][49][50][51][52][53][54][55][56], although the majority of these studies have design shortcomings and/or are limited by sample size. Importantly, follow-up of children born following embryo culture in either single or sequential media was not reported in any of the studies included in the present meta-analysis.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate. Included studies randomized either oocytes/zygotes or women. Eligible oocyte/zygote studies were analyzed to assess the risk difference (RD) and 95 % confidence intervals (CI) between the two media systems; eligible woman-based studies were analyzed to assess the risk ratio (RR) and 95 % CI for clinical pregnancy rate. Results No differences were observed between single and sequential media for either ongoing pregnancy per randomized woman (relative risk (RR) = 0.9, 95 % CI = 0.7 to 1.3, two studies including 246 women, I 2 = 0 %) or clinical pregnancy per randomized woman (RR = 1.0, 95 % CI = 0.7 to 1.4, one study including 100 women); or miscarriage per clinical pregnancy: RR = 1.3, 95 % CI = 0.4 to 4.3, two studies including 246 participants, I 2 = 0 %). Single media use was associated with an increase blastocyst formation per randomized oocyte/ zygote (relative distribution (RD) = +0.06, 95 % CI = +0.01 to Declaration of Authors' Roles Conception and design (CR, LR, WPM, IAS, and NR-F); search strategy (WPM, IAS); data extraction (WPM, PAN, IGRV, CON), analysis, and interpretation (all authors); writing the article (all authors). All authors had full access to all the data in the study and approved the final manuscript.Capsule In conclusion, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6 blastocyst stage. +0.12, ten studies including 7455 oocytes/zygotes, I 2 = 83 %) but not top/high blastocyst formation (RD = +0.05, 95 % CI = −0.01 to +0.11, five studies including 3879 oocytes/zygotes, I 2 = 93 %). The overall quality of the evidence was very low for all these four outcomes. Electronic supplementary materialConclusions Although using a single medium for extended culture has some practical advantages and blastocyst formation rates appear to be higher, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6. Future studies comparing these two media systems in well-designed trials should be performed.

show abstract

Section: Discussionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…These differences could be related with several aspects, among them the positive maternal effect due to the low number of litter size in VT litters compared to control litters and, probably, the embryo manipulation procedures. It is known that embryo manipulation occurring at preimplantation stages might alter future development because during this period, the embryo must undergo different events [38], affecting for instance the birth weight in human [18], or the appearance of "large offspring" phenotypes in cattle (for review see [25]), probably as a result of the impact of embryo manipulation on expression of some growthrelated imprinted genes. In fact, some re-searchers postulate that early exposure of mammals to stressful experiences could evoke some resilience to the effects of stressors [39] and, therefore, the early stress due to embryo manipulation could also act to adapt or prepare individuals for stressful environments.…”

Section: Discussionmentioning

confidence: 99%

“…Over the past decade, research works have questioned whether embryo cryopreservation and transfer procedures are neutral to survivors [16][17][18], as preimplantation em-bryos are removed from their natural environment and subjected to manipulation (cryopreservation and transfer procedures) which in fact affect their RNA expression [19,20], placental transcriptome, and proteome [21].…”

Section: Introductionmentioning

confidence: 99%

Embryo vitrification in rabbits: Consequences for progeny growth

et al. 2015

View full text Add to dashboard Cite

The objective of this research is to examine if there are any effects of the rederivation procedures on rabbit growth pattern and on weight of different organ in adults. For this purpose, three experiments were conducted on two different groups of animals (control group and vitrified-transferred group) to evaluate the possible effect of embryo manipulation (vitrification and transfer procedures) on future growth traits. The first experiment studies body weight from 1 to 9 weeks of age from the two groups. The second experiment describes the growth curve of progeny from experimental groups and analyzes their Gompertz curve parameters, including the estimation of adult body weight. The third experiment has been developed to study if there are any differences in different organ weight in adult males from the two experimental groups. In general, the results indicate that rederivation procedures had effect on the phenotypic expression of growth traits. The results showed that rabbit produced by vitrification and embryo transfer had higher body weight in the first four weeks of age than control progeny. Results from body weight (a parameter) and b parameter estimated by fitting the Gompertz growth curve did not show any difference between experimental groups. However, differences related with growth velocity (k parameter of the Gompertz curve) were observed among them, showing that the control group had higher growth velocity than the vitrified-transferred group. In addition, we found that liver weight at 40th week of age exhibits significant differences between the experimental groups. The liver weight was higher in the control males than in the VF males. Although the present results indicate that vitrification and transfer pro-cedures might affect some traits related with growth in rabbits, further research is needed to assess the mechanisms involved in the appearance of these phenotypes and if these phenotypes could be transferred to the future progeny.

show abstract

“…Errors in imprinting in domestic animals have been linked to culture conditions as well (Lonergan et al, 2003). Adverse effects of in vitro culture may also affect human development, with evidence that shorter culture times yield better longterm outcomes (Kallen et al, 2010;Kalra et al, 2012) and that culture media composition can affect birthweights (Dumoulin et al, 2010;Nelissen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Advances in quality control: mouse embryo morphokinetics are sensitive markers of in vitro stress

Wolff

Fredrickson

Walker

et al. 2012

Fertility and Sterility

View full text Add to dashboard Cite

what is known already: Current quality control testing methodology is sensitive but further improvements are needed to assure optimal culture conditions. MKs of embryo development may detect small variations in culture conditions. study design: Cross sectional-control versus treatment. Mouse embryo development kinetics of 466 embryos were analyzed according to exposure to various concentrations of toxins and toxic mineral oil. materials, setting, methods: Cryopreserved 1-cell embryos from F1 hybrid mice were cultured with cumene hydroperoxide (CH) (0, 2, 4, 6 and 8 mM) and Triton X-100 (TX-100; 0, 0.0008, 0.0012, 0.0016 and 0.002%). Using the Embryoscope, time-lapse images were obtained every 20 min for 120 h in seven focal planes. End-points were timing and pattern of cell division and embryo development. The blastocyst rate (BR) was defined as the percentage of embryos that developed to the expanded blastocyst stage within 96 h. main results and the role of chance: BR was not affected for embryos cultured in the three lowest concentrations of CH and the four lowest concentrations of TX-100. In contrast, a unique MK model detected all concentrations tested (P , 0.05). The MK model identified toxicity in two lots of toxic mineral oil that did not affect BR (P , 0.05).limitations, reasons for caution: A limited number of toxins were used so that the results may not apply to all potential embryo toxins. A larger sample size may also demonstrate other statistically significant developmental kinetic parameters.wider implications of the findings: MKs in mouse embryos are a sensitive and efficient method for quality control testing of in vitro culture conditions. BR, the end-point of traditional quality control assays, did not detect sublethal concentrations of toxins in the culture milieu in our study. This study demonstrates that temporal variation at key developmental stages reflects the quality of the culture environment. An MEA that incorporates MK will provide enhanced sensitivity and faster turn-around times.

show abstract

Further evidence that culture media affect perinatal outcome: findings after transfer of fresh and cryopreserved embryos

Cited by 182 publications

References 55 publications

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Embryo vitrification in rabbits: Consequences for progeny growth

Advances in quality control: mouse embryo morphokinetics are sensitive markers of in vitro stress

Contact Info

Product

Resources

About