Further Insights into Caprine Arthritis Encephalitis (CAE): The Current Status of Seroprevalence Among Small Ruminants in Two Selected States of Peninsular Malaysia

Paul, Bura Thlama; Hashi, Hamza Abdirahman; Burhannuddin, Nurul Najwa; Chung, Eric Lim Teik; Jesse, Faez Firdaus Abdullah; Lila, Mohd Azmi Mohd; Haron, Abd Wahid; Amat, Azuraida; Abba, Yusuf; Maqbool, Arsalan; Bhutto, Khaleeq ur Rehman; Isa, Kamarulrizal Mat; Amira, Nur Azhar; Odhah, Mohammad Naji; Hambali, Idris Umar; Norsidin, Mohd Jefri

doi:10.21315/tlsr2021.32.2.6

Cited by 4 publications

(1 citation statement)

References 33 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAE is characterized by lifelong persistence of the pathogen in monocytes and macrophages of the host [4][5][6] as well as variability in the incubation period and the induction of serologically detectable antiviral antibodies. CAE infection has been reported worldwide and is highly prevalent in countries that practice intensive production of dairy goats, such as Australia, USA, Canada, Japan, Norway, France and Switzerland [7].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Caprine Arthritis Encephalitis Virus from Animals in the Republic of Mordovia

Kolbasova¹,

Sevskikh²,

Titov³

et al. 2023

Animals

View full text Add to dashboard Cite

This article presents the results of virological and genetic studies of an isolate of caprine arthritis encephalitis (CAE) virus from the republic of Mordovia, Russian Federation. The isolate was found during monitoring studies of goat blood samples for the viral genome, and the presence of antibodies to lentiviruses was detected. According to the recommendation of the OIE, the positive result of PCR was confirmed with nucleotide sequencing. It was found that the obtained nucleotide sequence is identical to the genome of small ruminant lentiviruses presented in the GenBank database. Phylogenetic analysis showed that the isolate “Mordovia-2018” was included in the same cluster with an isolate from the Tver region of the Russian Federation detected in 2008. The sequence of the fragment of the env-gene of the isolate from the republic of Mordovia is available in GenBank under the number MN186380.1. To isolate the virus, a fraction of peripheral blood monocyte cells from the animal’s blood was added to a monolayer of lamb synovial membrane cell culture, and ten passages were carried out. The first manifestations of the cytopathic effect were observed after the third passage on the eighth day of cultivation in the form of single large cells of irregular shape with 5–7 nuclei. At the seventh passage, multiple syncytium with 7–12 nuclei were observed. At subsequent passage levels, the formation of syncytium containing more than 10–14 nuclei was observed.

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Caprine Arthritis Encephalitis Virus from Animals in the Republic of Mordovia

Kolbasova¹,

Sevskikh²,

Titov³

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

Seroprevalence and contributing factors of transboundary animal diseases in sheep and goats: a study in Peninsular Malaysia

Jimale,

Jesse,

Paul

et al. 2024

Trop Anim Health Prod

View full text Add to dashboard Cite

Validation of Brix refractometer to estimate immunoglobulin G concentration in goat colostrum

et al. 2021

View full text Add to dashboard Cite

Background and Aim: Immunoglobulin G (IgG) concentration is high in goat colostrum, particularly in the first few hours after parturition, and this is important for the kid's immunity and growth. IgG levels vary depending on several factors, including breed, disease status, colostrum management, handling, and collection time postpartum. A handheld optical refractometer, an affordable instrument that is simple to use in the field, is used widely in dairy farms to measure total solids. However, it can also be applied to estimate colostrum IgG content on the basis of comparison with standard measurement methods, usually radial immunodiffusion. Studies comparing %Brix values in relation to IgG concentration measured using enzyme-linked immunosorbent assay (ELISA) in goats are limited. The present study aimed to evaluate the use of a handheld optical Brix refractometer for the measurement of IgG concentration in goat colostrum, compare results with those using ELISA, and estimate the %Brix cutoff value equating to low-quality colostrum. Materials and Methods: Colostrum samples were collected on day 0 from 21 goats (nine Black Bengal, six Saanen, and six of their crossbred offspring) and were frozen. Subsequently, they were analyzed for IgG concentration using a goat-specific ELISA test and Brix percentage using a handheld refractometer. The optimum %Brix cutoff value for the evaluation of colostrum quality was evaluated. Results: The mean IgG concentration and %Brix in colostrum were 10.60±0.64 and 25.0±0.9 mg/mL, respectively. There was a significant (p<0.01) correlation between %Brix and IgG concentration. For an IgG concentration of 6.9 mg/dl, the cutoff value for %Brix was 18.5, equating to high specificity (100%) but low sensitivity (50%). A higher %Brix cutoff value of 21.5 showed high specificity (95%) and high sensitivity (100%). Conclusion: A Brix refractometer can be used to estimate goat colostrum quality with a proposed %Brix cutoff value of <18.5%-21.5% for poor-quality colostrum.

show abstract

Further Insights into Caprine Arthritis Encephalitis (CAE): The Current Status of Seroprevalence Among Small Ruminants in Two Selected States of Peninsular Malaysia

Cited by 4 publications

References 33 publications

Isolation and Identification of Caprine Arthritis Encephalitis Virus from Animals in the Republic of Mordovia

Isolation and Identification of Caprine Arthritis Encephalitis Virus from Animals in the Republic of Mordovia

Seroprevalence and contributing factors of transboundary animal diseases in sheep and goats: a study in Peninsular Malaysia

Validation of Brix refractometer to estimate immunoglobulin G concentration in goat colostrum

Contact Info

Product

Resources

About