Further isolation and characterization of temperature-sensitive mutants of influenza virus

Sugiura, Akira; Ueda, Masahiro; Tobita, Kiyotake; Enomoto, C.

doi:10.1016/0042-6822(75)90042-2

Cited by 100 publications

(83 citation statements)

References 26 publications

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“…after damage or mutation can be observed experimentally by complementation (Sugiura et al, 1972) and multiplicity reactivation (Barry, 1961). A second notable evolutionary advantage provided by a sexual mechanism such as segmentation is the acquisition of beneficial alleles.…”

Section: Genome Segmentation: a Mixed Blessingmentioning

confidence: 99%

Genome packaging in influenza A virus

Hutchinson

Kirchbach

Gog

et al. 2009

Journal of General Virology

258

279

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Section: Genome Segmentation: a Mixed Blessingmentioning

confidence: 99%

Genome packaging in influenza A virus

Hutchinson

Kirchbach

Gog

et al. 2009

Journal of General Virology

258

279

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

“…Mixed infection with two different strains of the same type results in high frequency recombination by genetic reassortment of genome segments (Sugiura, 1975). In spite of many similarities in biological properties and structure between type A and type B viruses, intertypic recombination has not been demonstrated (Sugiura, 1975). However, simultaneous infection with A and B type viruses results in interference with the multiplication of one or both viruses, depending on the multiplicity of infection (Gotlieb & Hirst, 1954;Tobita & Ohori, 1979;Mikheeva & Ghendon, 1982;Kaverin et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Interference Between Influenza A/WSN and B/Kanagawa Viruses

Aoki

Nishiyama

Tsurumi

et al. 1984

Journal of General Virology

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SUMMARYSimultaneous infection of MDCK cells with influenza viruses A/WSN and B/Kanagawa resulted in mutual interference with virus protein synthesis and in significant suppression of A/WSN growth. When infection by one virus preceded the other by 1 or 2 h, growth of the superinfecting virus was selectively inhibited at the level of transcription. Interference by the pre-infecting virus was strongly dependent on the expression of the viral genome but not on haemagglutinin activity. When the replication of both virus types was restricted to primary transcription by cycloheximide, the only translation products following removal of the drug were those of the preinfecting virus. This result was not affected by blocking secondary transcription by actinomycin D. These findings suggest that intertypic interference occurs at the level of primary transcription. This concept was supported further by the observation that a ts mutant of A/WSN (ts-65) with a defect in primary transcription interfered only with superinfection by B/Kanagawa at the permissive temperature.

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“…To date, ts mutants of orthomyxoviruses having a ts mutation in gene 7 coding for the M proteins, have been obtained only by one group of investigators (Sugiura et aL, 1975). According to their data, these mutants induced normal synthesis of virus-specific RNA and RNP, as well as synthesis of functionally active neuraminidase in infected cells under non-permissive conditions; however, synthesis of functionally active haemagglutinin was decreased (to 10%).…”

Section: Discussionmentioning

confidence: 99%

“…There are data on an influenza virus ts mutant having a mutation in gene 7 coding for M protein (Sugiura et al, 1975;Ritchey & Palese, 1977); however, the physiological defects of this mutant were not studied in detail.…”

Section: Introductionmentioning

confidence: 99%

Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein

Ghendon¹,

Markushin²,

Klimov³

et al. 1983

Journal of General Virology

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SUMMARYA fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP : M ratio as well as a decreased haemagglutinin content. A population of viri0ns formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.

show abstract

Further isolation and characterization of temperature-sensitive mutants of influenza virus

Cited by 100 publications

References 26 publications

Genome packaging in influenza A virus

Genome packaging in influenza A virus

Mechanism of Interference Between Influenza A/WSN and B/Kanagawa Viruses

Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein

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