Further isolation of AFLP and LMS markers for the mapping of the Ol-2 locus related to powdery mildew (Oidium neolycopersici) resistance in tomato (Solanum lycopersicum L.)

Ricciardi, Luigi M.; Lotti, Concetta; Pavan, Stefano; Bai, Yuling; Lindhout, P.; Giovanni, Claudio De

doi:10.1016/j.plantsci.2006.12.003

Cited by 6 publications

(5 citation statements)

References 59 publications

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“…All the ampliWcation products were subsequently digested with several restriction enzymes as described by Bai et al (2004). AFLP ® Wngerprints of the ol-2 linked markers identiWed by De Giovanni et al (2004) and Ricciardi et al (2007) were generated on a LICOR 4200 DNA sequencer as described by Bai et al (2003).…”

Section: Marker Analysismentioning

confidence: 99%

“…By screening the F 2 population of MM and R28 with the three polymorphic AFLP ® markers, the CAPS marker U3-2HhaI and the two SSR Tom316 and Tom332, a map of the ol-2 chromosomal region was constructed and integrated with the ol-2 maps reported by De Giovanni et al (2004) and Ricciardi et al (2007) (Fig. 2).…”

Section: An Integrated Map For the Chromosomal Region Harbouring Ol-2mentioning

confidence: 99%

“…Although marker assisted selection (MAS) with co-dominant molecular markers would allow the breeder to perform the selection of the ol-2 allele in backcross generations, the low genetic variation within S. lycopersicum (Miller and Tanksley 1990) hindered the identiWcation of polymorphic co-dominant markers linked to the resistance locus. So far, mainly ampliWed fragment length polymorphism (AFLP ® ) markers have been identiWed for the ol-2 region (De Giovanni et al 2004;Ricciardi et al 2007). In practical breeding, AFLP ® markers cannot oVer eYcient MAS as they are dominant and require advanced equipment.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, AFLP ® markers are patented and thus not utilizable by most breeding companies. Although several eVorts were made, the conversion of ol-2 linked AFLP ® markers into PCR markers failed (Ricciardi et al 2007). …”

Section: Introductionmentioning

confidence: 99%

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Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Pavan

Zhang²,

Borisova

et al. 2007

Euphytica

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The recessive gene ol-2 confers papillaassociated and race-non-speciWc resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identiWed two simple sequence repeat (SSR) markers and one cleaved ampliWed polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F 2 population to merge these markers with all the ol-2 linked ampliWed fragment length polymorphism (AFLP ® ) markers previously identiWed in an integrated genetic map. By screening a tomato bacterial artiWcial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation.Keywords Fluorescence in situ hybridization · Homology-based cloning · Integrated linkage map · Map-based cloning · Oidium neolycopersici · Solanum lycopersicum

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Section: Marker Analysismentioning

confidence: 99%

Section: An Integrated Map For the Chromosomal Region Harbouring Ol-2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Pavan

Zhang²,

Borisova

et al. 2007

Euphytica

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“…The ol-2 gene was discovered in an accession of S. lycopersicum var. cerasiforme (Ricciardi et al, 2007) and encodes a loss-offunction allele of a gene homologous to the Mlo gene of barley (Bai et al, 2008b). It confers race non-specific resistance through increased papillae formation and callose deposition at the site of the attempted penetration (Li et al, 2007).…”

Section: Tomato Responses To Combined Abiotic and Biotic Stressmentioning

confidence: 99%

Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

et al. 2018

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SUMMARYCrop plants are subjected to a variety of stresses during their lifecycle, including abiotic stress factors such as salinity and biotic stress factors such as pathogens. Plants have developed a multitude of defense and adaptation responses to these stress factors. In the field, different stress factors mostly occur concurrently resulting in a new state of stress, the combined stress. There is evidence that plant resistance to pathogens can be attenuated or enhanced by abiotic stress factors. With stress tolerance research being mostly focused on plant responses to individual stresses, the understanding of a plant's ability to adapt to combined stresses is limited. In the last few years, we studied powdery mildew resistance under salt stress conditions in the model crop plant tomato with the aim to understand the requirements to achieve plant resilience to a wider array of combined abiotic and biotic stress combinations. We uncovered specific responses of tomato plants to combined salinitypathogen stress, which varied with salinity intensity and plant resistance genes. Moreover, hormones, with their complex regulation and cross-talk, were shown to play a key role in the adaptation of tomato plants to the combined stress. In this review, we attempt to understand the complexity of plant responses to abiotic and biotic stress combinations, with a focus on tomato responses (genetic control and cross-talk of signaling pathways) to combined salinity and pathogen stresses. Further, we provide recommendations on how to design novel strategies for breeding crops with a sustained performance under diverse environmental conditions.

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Identification of Traits, Genes, and Crops of the Future

Pace

Ricciardi

Kumar

et al. 2013

Genomics and Breeding for Climate-Resilient Crops

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Studies on vulnerability and resilience of forest plant communities and crop species have multiplied with the growing realization that societal and scientific response is necessary to adapt to climate change impacts. The DPSIR (Driving forces, Pressure, State, Impact, Response) conceptual framework provides one of the simplest structure of indicators required to connect and model the dynamic systems of the causative and correlative components of climate envelopes and the genetic and genomic complexities regulating adaptive plant response to fluctuating environments and climate. Paleoclimate and vegetation type reconstruction from fossil records and species vicariance help in understanding the long-term dynamics of plant features and trait evolution associated with dispersal and climate changes. Comparative genomics demonstrated as alleles for those plant features (i.e., plant morphology and phenophase alteration), and for biotic (response to bacterial and fungal pathogens) and abiotic (i.e., drought, flooding) stress resistance are still part of the standing genetic endowment of the living gene pools of the crop and forest

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Further isolation of AFLP and LMS markers for the mapping of the Ol-2 locus related to powdery mildew (Oidium neolycopersici) resistance in tomato (Solanum lycopersicum L.)

Cited by 6 publications

References 59 publications

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew

Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

Identification of Traits, Genes, and Crops of the Future

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