The stable isotope composition of mercury (Hg) in a dated core from the anoxic zone of a saline, meromictic Arctic lake was found to vary as a complex function of the age and chemical composition of the sediment. Throughout the stratigraphic sequence, which spans the years 1899-1997, the ratios 198 Hg/ 202 Hg, 199 Hg/ 202 Hg, 200 Hg/ 202 Hg, 201 Hg/ 202 Hg, and 204 Hg/ 202 Hg expressed as δ-values (per mil deviations relative to a standard) reveal enrichment in 198 Hg, 199 Hg, 200 Hg, and 201 Hg, with depletion in 204 Hg, the degree of enrichment varying inversely with atomic mass. A plot of δ 198 Hg, δ 199 Hg, δ 200 Hg, and δ 201 Hg against depth gave parallel profiles characterized by large, regular undulations superimposed on an overall trend toward increase with depth (i.e. age), and the δ 204 Hg profile is a mirror image of them. The δ 198 Hg, δ 199 Hg, δ 200 Hg, and δ 201 Hg values of the oldest (1899-1929) strata vary inversely with NH 2 OH‚HCl/HNO 3 -extractable manganese concentration, but those of the youngest strata give a positive correlation; intermediate strata show no correlation and negligible variation in δ-values, possibly signifying a transition phase in which the two opposite trends offset each other. The δ-values show similar but weaker relationships with organic carbon. The results strongly suggest fractionation of Hg isotopes by microbial activities linked to oxidation-reduction reactions in the lake, although effects of isotopic signatures indicative of the sources of the Hg have not been ruled out. The radical change in the nature of the relationship between δ-values and sediment chemistry over time may reflect environmental and biotic changes that altered the isotope-fractionating processes. These findings imply that variations in the isotopic makeup of Hg, together with related physical, chemical, and biological data, could yield important new information about the biogeochemical cycle of Hg.