Further observations of corynebacterium renale as an indicator organism in the detection of the exfoliation-positive strains of Staphylococcus aureus

Skalka, Boris; Pillich, J; Pospíśil, L

doi:10.1016/s0174-3031(83)80066-3

Cited by 19 publications

(14 citation statements)

References 11 publications

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“…The antimicrobial activity depends on the sensitivity of the indicator strains, and the activity was spanning between 100 AU/ml using the least sensitive indicator strain and 64,000 AU/ml when the most sensitive indicator strain was used (by the micro-titer assays). The inhibitory zones varied between 10 and 20 mm when the antimicrobial potency was tested on agar plates by Skalka [35]. The antimicrobial activity produced by the AL41 strain was lost after the treatment by proteases, which strongly suggested that the activity was due to the proteina- ceous substance, most probably a bacteriocin, and consequently that substance was puriWed and characterized.…”

Section: Resultsmentioning

confidence: 99%

“…The bacteriocin activity was determined by three methods; by detection of clear zone around indicator organisms [35], by critical dilution method on micro titer plates [16] and by the agar spot test [10]. One bacteriocin unit was deWned as the amount of bacteriocin causing 50% growth inhibition (50% of the turbidity of the control culture without bacteriocin) or in AU/ml (arbitrary units per ml-the reciprocal of the highest twofold dilution demonstrating complete inhibitory activity of the indicator strain).…”

Section: Bacterial Cultures Media and Growth Conditionsmentioning

confidence: 99%

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Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41

Mareková¹,

Lauková²,

Skaugen³

et al. 2007

J Ind Microbiol Biotechnol

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The new bacteriocin, termed enterocin M, produced by Enterococcus faecium AL 41 showed a wide spectrum of inhibitory activity against the indicator organisms from diVerent sources. It was puriWed by (NH 4 ) 2 SO 4 precipitation, cation-exchange chromatography and reverse phase chromatography (FPLC). The puriWed peptide was sequenced by N-terminal amino acid Edman degradation and a mass spectrometry analysis was performed. By combining the data obtained from amino acid sequence (39 Nterminal amino acid residues was determined) and the molecular weight (determined to be 4 628 Da) it was concluded that the puriWed enterocin M is a new bacteriocin, which is very similar to enterocin P. However, its molecular weight is diVerent from enterocin P (4 701.25). Of the Wrst 39 N-terminal residues of enterocin M, valine was found in position 20 and a lysine in position 35, while enterocin P has tryptophane residues in these positions.

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Section: Resultsmentioning

confidence: 99%

Section: Bacterial Cultures Media and Growth Conditionsmentioning

confidence: 99%

Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41

Mareková¹,

Lauková²,

Skaugen³

et al. 2007

J Ind Microbiol Biotechnol

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“…Bacteriocinogenic activity was assessed by a qualitative method according to Skalaka, Pillich, and Pospíšil (1983). E. avium EA5 (isolated from piglets by Strompfová & Lauková, 2007), L. monocytogenes CECT 934 and S. aureus ATCC 29213 were used as indicators for bacteriocinogenic potential assessment.…”

Section: Assessment Of Bacteriocinogenic Potentialmentioning

confidence: 99%

Assessment of Coagulase‐Negative Staphylococci and Lactic Acid Bacteria Isolated from Portuguese Dry Fermented Sausages as Potential Starters Based on Their Biogenic Amine Profile

Alfaia

Gouveia

Fernandes

et al. 2018

Journal of Food Science

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The selection of coagulase-negative staphylococci and lactic acid bacteria (LAB) isolates were based on their production of biogenic amines in order to avoid this potential hazard production in meat products. The most suitable isolates could be used as safe starter cultures in meat products industry. The staphylococci and LAB selected will achieve particular organoleptic characteristics in meat products and bioprotection from pathogens.

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“…The detection of inhibitory activity was performed using an agar diffusion method (Skalka et a / . 1983).…”

Section: E T E C T I O N O F I N H I B I T O R Y a C T I V I T Ymentioning

confidence: 99%

Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231

Lauková

Mareková

Javorský

1993

Lett Appl Microbiol

107

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Further observations of corynebacterium renale as an indicator organism in the detection of the exfoliation-positive strains of Staphylococcus aureus

Cited by 19 publications

References 11 publications

Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41

Isolation and characterization of a new bacteriocin, termed enterocin M, produced by environmental isolate Enterococcus faecium AL41

Assessment of Coagulase‐Negative Staphylococci and Lactic Acid Bacteria Isolated from Portuguese Dry Fermented Sausages as Potential Starters Based on Their Biogenic Amine Profile

Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231

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