In this paper, miniaturized dual and tri-band slot antenna design for various applications (GPS, MSS, cordless telephone, radio astronomy) is presented. For these proposed dual and tri-band miniaturized antennas, the first resonant is achieved by the reference slot antenna, loaded with six slits on both the end of slot which is antenna 1, the second resonant is achieved by loading with three circular loops on the middle of slot which is antenna 2, a third resonant frequency is obtained by loading with nine metallic strips on back side of the substrate which is antenna 3. The combination of antenna 1 and 2 constructs a dual-band antenna that resonates at frequencies of 1.91 and 3.32 GHz, respectively. As results, reduction in resonant frequency of 41.94% with enhanced impedance bandwidth of 117.18% is achieved for first resonance frequency in comparison with reference antenna for dual-band antenna. Then, combining antenna 1, 2 and 3 construct a tri-band antenna which resonates at frequencies of 1.55, 2.64 and 3.81 GHz, respectively. Hence, it is achieved reduction in resonant frequency of 52.88% with enhanced impedance bandwidth of 59.01% for first resonance frequency and 19.75% in reduction resonance frequency for second resonance frequency in comparison with reference antenna.