Further Studies of Genetic Variation in Schistosoma mansoni

LoVerde, Philip T.; DeWald, John; Minchella, Dennis J.

doi:10.2307/3281704

Cited by 13 publications

(4 citation statements)

References 4 publications

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“…RAPD primers survey a large number of loci throughout the entire genome and have proven useful in characterizing both inter- and intraspecific relationships [14, 15]. As very little DNA is required for such analyses, cercarial or miracidial stages of schistosomes can be used as a source of genetic material, thus minimizing selection caused by passaging through unnatural hosts [13, 16]. …”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Schistosoma haematobium parasite IS NOT associated with severity of disease in an endemic area in Sudan

et al. 2014

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BackgroundOver 650 million people globally are at risk of schistosomiasis infection, while more than 200 million people are infected of which the higher disease rates occur in children. Eighty three students between 6-20 years (mean 12.45 ± 3.2) from Quran School for boys in Radwan village, Gezira state were recruited to investigate for the relationship between the genetic diversity of Schistosoma haematobium strains and the severity of the disease.MethodSchistosoma haematobium infection was detected by filtration of urine. Ultrasonography was done on each study subject, while PCR technique was used for genotyping via random amplified polymorphic DNA (RAPD) with A01, A02, A12, Y20 and A13 primers. A01 primer gave three different genotypes (A01-1, A01-2 and A01-3).ResultsAbout 54.2% (45/83) were S. haematobium egg positive by urine filtration. On assessment of the upper and lower urinary tract by ultrasound technique, 61.4% (51/83) were positiveand73.3% (60/83) samples were PCR positive. No significant difference was found when comparing the three different genotypes with severity of the disease.ConclusionThis study concludes that no association was found between the different genotypes of S.haemtobium and the severity of the disease. Examination of more samples from different areas to identify any possible differences between the parasites genes and disease severity was recommended.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2334-14-469) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of Schistosoma haematobium parasite IS NOT associated with severity of disease in an endemic area in Sudan

et al. 2014

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“…In the Schistosoma japonicum-group, relatively large interspecies variation occurs but apparently extremely limited intrapopulation variation (FLETCHER et al, 1980;WOODRUFF et al, 1987). Greater intrapopulation variation but little interpopulation variation seems to occur in S. mansoni (FLETCHER et al, 1981;LOVERDE et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Enzyme polymorphism in Schistosoma mattheei from cattle in the Eastern Transvaal Lowveld

Kruger

1989

J. Helminthol.

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Enzyme electrophoresis was conducted on 10 Schistosoma mattheei adult worm samples, comprising 270 individuals, collected from cattle in the Eastern Transvaal Lowveld. Glucose-6-phosphate dehydrogenase (G6PDH) was studied in all the samples and phosphoglucomutase (PGM) and malate dehydrogenase (MDH) in five populations each. Only one population was polymorphic for G6PDH. In this population, in addition to the allele found in all the other samples, a second allele occurred with a similar Rf value to S. haematobium. The two alleles were in Hardy-Weinberg equilibrium. MDH-1 exhibited two alleles. However, these alleles were not in equilibrium. In certain populations, heterozygotes occurred together with homozygotes of one of the alleles only. PGM was monomorphic in all the populations studied.

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“…With such biotechnology, it became feasible to investigate population genetics of schistosomes with few available sequence data. Molecular markers perform on a whole genome scan, is capable of identifying a large number of loci and have emerged as a powerful tool in distinguishing both inter and intraspecific variations" [13]. The realization of the enormous influence that intraspecific variation may have on the epidemiology of parasitic disease has resulted in a great deal of research into reliable methods for identification and characterizing strains.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Molecular Characterization of Dral 1 gene of Schistosoma haematobium Associated with Pathogenesis of Human Urinary Schistosomiasis among School Pupils in Cross River State, Nigeria

Osondu-Anyanwu

Edema

Nkang

et al. 2022

ARRB

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Urinary schistosomiasis which is transmitted by schistosome species is the major cause of liver and bladder pathologies and still remains a serious threat in the underdeveloped and developing world. This study evaluates the prevalence of Schistosoma haematobiuminfection among school aged children in Biase, Obubra and Ogoja Local Government Areas of Cross River State. Five hundred (500) pupils were examined and selected randomly from a public primary and secondary schools in the study area. Freshly passed mid-day urine samples were collected and transferred to the laboratory where there were examined for the presence of Schistosoma haematobium eggs. Study participants were grouped into three age groups,8-10 years. 11-13 years, and 14-16 years old. Overall prevalence of S. heamatobium was (13.6%). Infection was more prevalent among the age group of 14-16years, the percentage of prevalence and intensity of infection were higher in males (14.1%) than in females (6.9%). Inter simple sequence repeats of PCR test performed for the collected urine samples using ISSR test of the Dral-1 gene reveals 73% study subjects had a polymorphism for UPA02 and UPA13 primers, while primer UPA13 showed 24% polymorphism. Total number of polymorphic bands were 2 each for primers UPA02 and UPA13 primers while UPA12 showed only one polymorphic band. Major allele frequencies (MAF) were 0.53 for each of UPA02 and UPA 13 primers but showed 0.71 frequency with UPA12 primer. Allele frequencies (AF) also varied slightly among the primers used. UPA02 and UPA 13 had allele frequencies of 8 each while UPA12 had 4 allele frequencies (Table 13). Nei’s genetic diversity indices for the primers revealed variations among the different primers. UPA02 and UPA13 Nei’s gene diversity of 0.64 each while primer UPA12 showed gene diversity of 0.28. Results of polymorphic information content showed that primers UPA02 and UPA13 discriminately revealed a PIC of 0.68 while UPA12 discriminated 0.28 PIC. This study therefore, revealed a critical need for targeting health campaign towards school age children and heads of households in order to empower them with the basic knowledge to recognize, treat and manage their health challenges.Applications of one to two doses of praziquantel considerably reduced the severity of urinary Schistosomiasis in the study area.

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Further Studies of Genetic Variation in Schistosoma mansoni

Cited by 13 publications

References 4 publications

Genetic diversity of Schistosoma haematobium parasite IS NOT associated with severity of disease in an endemic area in Sudan

Genetic diversity of Schistosoma haematobium parasite IS NOT associated with severity of disease in an endemic area in Sudan

Enzyme polymorphism in Schistosoma mattheei from cattle in the Eastern Transvaal Lowveld

Prevalence and Molecular Characterization of Dral 1 gene of Schistosoma haematobium Associated with Pathogenesis of Human Urinary Schistosomiasis among School Pupils in Cross River State, Nigeria

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