Further studies of Olpidium as a vector of tobacco necrosis virus

Teakle, D. S.; Gold, A. H.

doi:10.1016/0042-6822(63)90069-2

Cited by 43 publications

(16 citation statements)

References 2 publications

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“…Dang. (Teakle and Gold, 1963). The effects of two different soil biota, an endophytic plant-pathogenic nematode and arbuscular mycorrhiza are reported in a single publication (Bonte et al, 2010) and are thus represented as two entries in table 1.…”

Section: Going Belowgroundmentioning

confidence: 99%

Plant-mediated aboveground-belowground interactions: the spider mite perspective

Hoffmann¹,

Schausberger²

2012

Acarologia

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Section: Going Belowgroundmentioning

confidence: 99%

Plant-mediated aboveground-belowground interactions: the spider mite perspective

Hoffmann¹,

Schausberger²

2012

Acarologia

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“…Olpidium 1 has been maintained free from TNV for the past 2 years but, although recultured many times, still carries big-vein virus. Teakle & Gold (1963) reported that virus transmission was prevented by an antiserum to TNV diluted 1/50, when mixed with virus before zoospores were added, but not after. They concluded from this 'that the Olpidium zoospore acquires and harbours TNV in such a manner that antiserum cannot inactivate it.…”

Section: Freeing Olpidium Cultures From Contamination With Virusmentioning

confidence: 99%

Transmission of Tobacco Necrosis Virus by Zoospores of Olpidium brassicae

Kassanis

Macfarlane

1964

Journal of General Microbiology

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SUMMARYStrain D of tobacco necrosis virus (TNV) was transmitted by zoospores of 3 different isolates of Olpidium brassicae (Wor.) Dang. to roots of Mung bean and lettuce grown in modified Hoagland's solution diluted 1/20. On Mung bean roots necrotic local lesions formed one day after exposure to virus and zoospores. Virus in lettuce was assayed by inoculation to leaves of French bean. Virus transmission was favoured by decreasing salt concentration and increasing the pH value of the nutrient solution and depended also on the concentrations of virus and zoospores. With 105 zoospores/ml. transmission to lettuce was obtained with as little virus as 0.05 ,ug./l. When the virus concentration was 5 ,ug./l., 50-100 zoospores/ ml. were effective. Fungus infection as measured by the number of zoosporangia in the root was not strictly correlated with virus infection.Exposure of roots to virus + zoospore mixture for 1 min. sufficed to infect them with virus. More transmission occurred when virus was added before or together with zoospores, than after. Roots, exposed to zoospores for 10 min., then washed, were more readily infected by TNV when virus was introduced during the first hour or two after zoospore attachment to the root cells than later; there was some transmission even when virus was withheld till 4 hr after washing. Immersing roots, inoculated with fungus and virus, in hot water (60') killed the fungus but not the virus, and varying the interval between inoculation and heating showed that virus became established after 2-3 hr.Isolates of Olpidium brassicae naturally contaminated by strain D or A of TNV were freed from contamination by inoculating lettuce roots with dilute zoospore suspensions. Zoospores mixed or naturally contaminated with TNV were partially separated from it by centrifugation. Virus transmission was prevented by adding concentrated homologous antiserum to zoospores that had already been exposed to virus, or by adding very dilute antiserum to virus before mixing it with zoospores. The extent to which transmission was prevented by antisera to other strains of TNV depended on the degree of their serological relationship to strain D. The present evidence does not support the suggestion that TNV is carried inside the fungus.

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“…No virus was detected when CSNV alone, Olpidium zoospores alone, or buffer alone had been added to pots of young cucumber plants. These results indicate that the cucumber virus being investigated is readily transmitted by Olpidium zoospores, as was found for TNV by Teakle (1962); Teakle and Gold (1963); and Kassanis and Macfarlane (1964).…”

Section: Transmission By Olpidium Brassicaementioning

confidence: 50%

Cucumber systemic necrosis caused by a strain of tobacco necrosis virus

Thomas

Fry

1972

New Zealand Journal of Agricultural Research

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Cucumber systemic necrosis virus (CSNV), a strain of tobacco necrosis virus (TNV), was found occurring naturally on glasshouse cucumbers plan.cd during the winter, and was transferred experimentally to produce symptoms in cucumber, french bean, cowpea, and tobacco.The virus was transmitted from cucumber to seedlings of cucumber and lettuce using isolates of Olpidium brassicae (Wor.) Dang. from lettuce as the vector. Light microscope studies revealed high concentrations of Olpidium sporangia and resting spores within roots of naturally infected cucumber plants.The host range, physical properties, serology. and electron microscopy of the virus identify it as cucumber systemic necrosis virus. a strain of tobacco necrosis virus. This is the first record of cueumber systemie necrosis virus in New Zealand.

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Further studies of Olpidium as a vector of tobacco necrosis virus

Cited by 43 publications

References 2 publications

Plant-mediated aboveground-belowground interactions: the spider mite perspective

Plant-mediated aboveground-belowground interactions: the spider mite perspective

Transmission of Tobacco Necrosis Virus by Zoospores of Olpidium brassicae

Cucumber systemic necrosis caused by a strain of tobacco necrosis virus

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