Further studies on antigen variation in Staphylococcus aureus

Pereira, A. Torres

doi:10.1128/jcm.13.2.245-247.1981

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…These strains were subcultured for 1 to 5 months before being tested with crude L. polyphemus lectin and may have undergone surface modification. Antigen variation has been reported to occur in S. aureus (27). Some of the subcultured S. aureus strains developed autoagglutination in PBS, and the tests with L. polyphemus lectin could not be interpreted.…”

Section: Discussionmentioning

confidence: 98%

Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins

Davidson¹,

Keller²,

Doyle³

1982

J Clin Microbiol

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MATERIALS AND METHODS Reagents. All chemicals, salts, and sugars were of the highest grade available. L. polyphemus (crude), T. vulgaris (pure), and other routinely used lectins were purchased from E-Y Laboratory (San Mateo, Calif.). M. indica extract was prepared from the dried seeds of commercially purchased mangoes. The seeds were pulverized in a micromill (Chemical Rubber Co., Columbus, Ohio) and extracted for 2 h at room temperature in 10 times their weight of phosphate-buffered saline (PBS: 0.05 M potassium phosphate, 0.15 M 547

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Section: Discussionmentioning

confidence: 98%

Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins

Davidson¹,

Keller²,

Doyle³

1982

J Clin Microbiol

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Plasmid analysis of 219 methicillin-resistant Staphylococcus aureus strains with uncommon profiles isolated in Lisbon

Melo‐Cristino

Pereira

1989

Journal of Hospital Infection

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The Effect of the Repeated Subcultures of Helicobacter pylori on Adhesion, Motility, Cytotoxicity, and Gastric Inflammation

et al. 2002

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In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H. pylori SS1 strain was subcultured 64 times on agar plates containing Brucella broth and 5% bovine calf serum. The adhesion, motility, cytotoxicity, and gastric inflammation produced in Mongolian gerbils were compared between the first and 64th subcultured strain. The adhesion rates, following 3 hr exposure of AGS cells to either the first strain or the 64th-transferred strain, were 21% and 12%, respectively. The motility of the 64th-transferred strain decreased significantly when compared to the 1st strain (9.1 mm vs. 15.1 mm). The cytotoxicity index tended to be higher in the first strain than in the 64th-transferred strain (73.7% vs. 69.2%). The initial infection rate on the gerbils showed no difference between the two strains. However, chronic gastric inflammation of the first strain-infected gerbils was somewhat more severe than that of the 64th-transferred strain-infected gerbils. Therefore, the use of repeatedly subcultured strains of H. pylori in virulence experiments can lead to different results from thoses of the original strain.

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Further studies on antigen variation in Staphylococcus aureus

Cited by 3 publications

References 10 publications

Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins

Differentiation of coagulase-positive and coagulase-negative staphylococci by lectins and plant agglutinins

Plasmid analysis of 219 methicillin-resistant Staphylococcus aureus strains with uncommon profiles isolated in Lisbon

The Effect of the Repeated Subcultures of Helicobacter pylori on Adhesion, Motility, Cytotoxicity, and Gastric Inflammation

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