Further studies on light and sporulation in Botrytis cinerea

Locus of Blue and Near Ultraviolet Reversible Photoreaction in the Stages of Conidial Development in Botrytis cinerea

“…reversible photoreaction, plays an important role in the photocontrol of conidial development in Helminthosporium oryzae (Honda, Sakamoto & Oda, 1968). This system has since been found during conidiation in Alternaria tomato (Kumagai & Oda, 1969, 1973Kumagai, Yoshioka & Oda, 1976) and Botrytis cinerea (Tan & Epton, 1974). We suggested that the developmental stage in which the photosystem participates might be the maturation of the conidiophore.…”

Blue-light Inhibition of Sporulation in Botrytis cinerea

“…These results may give further insight into the action mechanism of the hypothetical receptor pigment, mycochrome (Honda, 1969;Kumagai & Oda, 1969b; It has already been suggested that blue light converts the M, form, which is effective for sporulation, to MNllV, the form that is not active for sporulation (Tan & Epton, 1974). The fact that blue light must be applied continuously and for long duration to obtain almost total inhibition of sporulation suggests that: (i) the quantum efficiency for the conversion of M, to My,, is lower than the reverse process; (ii) the conversion may involve more reaction steps, some of which may be time-dependent; and (iii) M,,, or some intermediate(s) in the photo-transformation M, -+ M,,, may be unstable and revert back to M, when light is removed, thus causing some spores to be formed.…”

“…(Bjornsson, (Tan & Epton, 1973). Suppression of sporulation by blue light after photoinduction has been shown for Alternaria tomato (Aragaki, 1962; Kumagai & Oda, 1969b), Alternaria solani (Lukens, 1963), Helminthosporium oryzae , Stemphylium botryosum (Leach, I 968), Stemphylium solani (Sproston, 1971), and B. cinerea (Tan & Epton, 1974). This suppression of sporulation by blue light was in fact a reversal of the potentiated sporulation (Honda, Sakamoto & Oda, 1968; Kumagai & Oda, 1969b;Tan & Epton, 1974).…”

Red-Far-red Reversible Photoreaction in the Recovery from Blue-light Inhibition of Sporulation in Botrytis cinerea

“…The same isolate of Botrytis cinerea as in Tan & Epton (1973,1974 was used. Cultures were incubated in the dark at 2 0 + I "C for 44 days before use in the irradiation experiments.…”

“…Cultures were incubated in the dark at 2 0 + I "C for 44 days before use in the irradiation experiments. Detailed procedures for culturing, the light sources and filters used, and the method for quantification of spore production, have already been described (Tan & Epton, 1973, 1974.…”