Further studies on the rhamnomannans and acidic rhamnomannans of Sporothrix schenckii and Ceratocystis stenoceras

Gorin, Philip A.J.; Haskins, R. H.; Travassos, Luiz R.; Mendonça-Previato, Lúcia

doi:10.1016/s0008-6215(00)84440-7

Cited by 54 publications

(31 citation statements)

References 32 publications

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“…The 13 C-NMR spectrum of the acid-degraded polysaccharide is in excellent agreement to reported values of a synthetic (Ogawa and Sasajima 1981) and a yeast α-(1,6)-mannan (Kogan et al 1988). The spectrum (recorded at 70°C) of a similar polysaccharide, which was isolated following Smith degradation of a glucuronorhamnomannan from Ceratocystis stenoceras, also compares favorably with our data (Gorin et al 1977), and one of the two water-soluble cell wall polysaccharides from Talaromyces flavus contains a similar α-(1,6)-mannan (Parra et al 1994). The structural elucidation of the side-chain constituents (including the uronic acid residue), sequence, and linkage pattern requires selective chemical degradations and is a matter of further investigation.…”

Section: Nmr Analysis Of the Heteroglycansupporting

confidence: 83%

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Rath¹,

Messner

Kosma

et al. 1995

Archives of Microbiology

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A heteroglycan responsible for the binding of the enzyme beta-1,4-D-glucosidase (EC 3.2.1.21) to fungal cell walls was isolated from cell walls of the filamentous fungus Trichoderma reesei. The heteroglycan, composed of mannose, galactose, glucose, and glucuronic acid, also activated beta-1,4-D-glucosidase, beta-1,4-D-xylosidase and N-acetyl-beta-1,4-D-glucosaminidase activity in vitro. The structural backbone of this heteroglycan was prepared by acid hydrolysis and gel filtration. The molecular structure of the core of the heteroglycan was determined by NMR studies as a linear alpha-1,6-D-mannan. The mannan core obtained by acid degradation stimulated the beta-glucosidase activity by 90%. Several glycosidases from Aspergillus niger were also activated by the T. reesei heteroglycan. The beta-glucosidase of Trichoderma was activated by mannan from Saccharomyces cerevisiae to a comparable extent.

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Section: Nmr Analysis Of the Heteroglycansupporting

confidence: 83%

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Rath¹,

Messner

Kosma

et al. 1995

Archives of Microbiology

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“…The results suggest the gp84 seems to be acidic in that once it was identified in a cell wall fraction (fraction A) which is directly precipitated by Cetavlon. Glucuronic acid has been described for S. schenckii O-linked oligosaccharides, isolated from CWPR after fl-elimination [10] and also for the rhamnomannan fraction isolated from the S. schenckii yeast cells of 1099-18 strain by strong alkaline treatment (KOH 2% at 100 °C) [23].…”

Section: Kmentioning

confidence: 99%

Identification of a concanavalin A-binding antigen of the cell surface ofSporothrix schenckii

Lima

Bezerra

1997

Med Mycol

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Sporothrix schenckii (1099-18) cell wall peptido-rhamnomannan (CWPR) was fractionated by affinity chromatography with Concanavalin A. The Con A-bound and Con A-unbound fractions were probed with an anti-S, schenckii rabbit serum. We identified within the Con A-bound fraction three main antigens with approximate molecular weights of 84, 70 and 58 kDa. Glycopeptide fl-elimination reduced rabbit antiserum reactivity for the 84 kDa antigen (gp84) with concomittant enhanced reactivity for the 70 kDa antigen (gp70). By Western blot with Con A-HRP conjugate we demonstrated that gp84 strongly reacted with this lectin and this was the predominant antigen identified. The gp84 antigen was also demonstrated to be present on other S. schenckii strains.

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“…schenckii cell wall seems to be a rhamnomannan isolated by alkaline extraction (63,180), or complexed as a peptidorhamnomannan after extraction by milder methods (100,155). The rhamnomannans extracted with alkali were composed of subunits I and II (Fig.…”

Section: Sporothrixschenckiimentioning

confidence: 99%

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

San-Blas

1982

Mycopathologia

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The ability of some fungi to cause disease in animals, and particularly in humans, appears to be related to some peculiar trait of their metabolism not shared by taxonomically related species, so that among thousands of species of fungi, only some 100 are considered pathogenic for humans.Recently, there has been an increase in the basic research aimed at elucidating the physiology, biochemistry, and mechanisms of pathogenicity of the fungi in addition to the responses of the infected host. The results from these studies are often difficult to compare because of the lack of reference cultures, and because variability among laboratories are found for many parameters such as origin of isolates, growth conditions, and fractionation procedures used to obtain chemically defined components of cell walls.This review deals with cell wall structures and their influence in host-parasite relationships in those relatively few pathogenic fungi on which substantial literature exists. Serological properties of the walls are included when available. Readers interested in medical and biological aspects of fungi may consult Rippon's (140) and Emmons et al.'s (51) textbooks, among others. Some reviews on microbial surfaces and their relationships to pathogenicity have been published (160 162)2The pathogenic fungi included in this review are classified according to their cell wall structure, following Bartnicki-Garcia's criteria (15) on the distribution of the two major polysaccharides within the fungal cell wall. This classification is based on the fact that fungi may be subdivided into various categories according to the chemical nature of their walls, and that these categories closely parallel conventional taxonomic boundaries. As seen in Table l, this classification is based on dual combinations of those polysaccharides which appear to be the principal component of vegetative walls, disregarding the presence of small amounts of other classifying polysaccharides. The few pathogenic fungi whose cell walls have been studied to some extent belong to Bartnicki-Garcia's categories 5 (chitin-glucan) and 6 (mannan-glucan) ( Table 2). Besides these main polysaccharides, some minor components of the cell wall may also play important roles in pathogenicity, as antigens, or as virulence factors, and they will be mentioned in this review. DermatophytesDermatophytes are a highly specialized group of fungi that, through long evolutionary processes became adapted to invade, colonize, and nourish themselves on the keratinized tissues of animals. The three genera of dermatophytes are: Trichophyton, Microsporum, and Epidermophyton, of which 21, 15, and 1 species, respectively, are currently considered valid members (1) (ln the Vth Panamerican Conference on Mycoses, April 1980, Caracas, these figures were raised to 27, 16, and 2, respectively). Mating studies have revealed that, for some cases, what were considered as single species of Microsporum and Trichophyton, actually represent complexes of more than one species, indis- Mycopathologia7...

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Further studies on the rhamnomannans and acidic rhamnomannans of Sporothrix schenckii and Ceratocystis stenoceras

Cited by 54 publications

References 32 publications

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

The α- d -mannan core of a complex cell-wall heteroglycan of Trichoderma reesei is responsible for β-glucosidase activation

Identification of a concanavalin A-binding antigen of the cell surface ofSporothrix schenckii

The cell wall of fungal human pathogens: Its possible role in host-parasite relationshipsA review

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