Further study on the changes in the concentration of prolactin-binding sites in different organs of Xenopus laevis male and female, kept under dry conditions and then returned to water (their natural habitat)

Muccioli, Giampiero; Guardabassi, A.; Pattono, Paola Maria; Genazzani, E

doi:10.1016/s0016-6480(89)80039-5

Cited by 11 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In R. esculenta, PRL binding to skin fractions was generally high in the month of October, whereas the lowest value was found during the summer season (D'Istria et al 1987). In X. laevis, the values of PRL specific binding to the membranes from the skin were decreased by dehydration of the animals (Muccioli et al 1989). Yamamoto et al (2000) investigated the tissue distribution of PRLR mRNA in adult and larval X. laevis by northern hybridization.…”

Section: Discussionmentioning

confidence: 95%

“…PRLR has been observed in skin of Xenopus laevis Daudin, 1802 and Rana esculenta L., 1758 by using 125 I-PRL binding (D'Istria et al 1987;Muccioli et al 1989). In R. esculenta, PRL binding to skin fractions was generally high in the month of October, whereas the lowest value was found during the summer season (D'Istria et al 1987).…”

Section: Discussionmentioning

confidence: 99%

“…In adult amphibian skin, PRLR has been detected by using different methods (D'Istria et al 1987;Muccioli et al 1989;Yamamoto et al 2000;Li et al 2006), although there is a lack of knowledge concerning localization of PRLR in the skin components. In this study, by using an immunohistochemical technique, we have investigated the localization and distribution of PRLR in the dorsal and ventral skin of Rana ridibunda Pallas, 1771 and discussed possible functions of PRL in different skin regions of the body.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Localization of prolactin receptor in the dorsal and ventral skin of the frog (Rana ridibunda)

et al. 2010

View full text Add to dashboard Cite

Abstract:The dorsal and ventral skin in amphibians plays an important role in osmoregulation. Prolactin hormone is involved in regulation of amphibian skin functions, such as water and electrolyte balance. Therefore, amphibians may be useful as a model for determining the sites of the prolactin receptor. In this study, prolactin receptor was detected in frog dorsal and ventral skin using immunohistochemical staining method. Prolactin receptor immunoreactivity was localized in all epidermal layers except stratum corneum of dorsal skin epidermis, stratum germinativum layer of ventral skin epidermis, myoepithelial cells, secretory epithelium and secretory channel cells of granular glands in both skin regions. The mucous glands and secretory granules of granular glands did not show immunoreactivity for the prolactin receptor. According to our immunohistochemical results, the more widespread detection of prolactin receptor in dorsal skin epidermis indicates that prolactin is more effective in dorsal skin. Presence of prolactin receptors in epidermis points out its possible osmoregulatory effect. Moreover, detection of receptor immunoreactivity in various elements of poison glands in the dermis of both dorsal and ventral skin regions suggests that prolactin has a regulatory effect in gland functions.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Localization of prolactin receptor in the dorsal and ventral skin of the frog (Rana ridibunda)

et al. 2010

View full text Add to dashboard Cite

show abstract

Biochemical study of prolactin binding sites in Xenopus laevis brain and choroid plexus

1990

View full text Add to dashboard Cite

The occurrence of prolactin binding sites in some brain structures (telencephalon, ventral hypothalamus, myelencephalon, hypophysis, and choroid plexus) from Xenopus laevis (anuran amphibian) was studied by the in vitro biochemical technique. The higher binding values were obtained at the level of the choroid plexus and above all of the hypothalamus. On the bases of hormonal specificity and high affinity, these binding sites are very similar to those of prolactin receptors of classical target tissues as well as of those described by us in other structures from Xenopus. To our knowledge, the present results provide the first demonstration of the occurrence of prolactin specific binding sites in Xenopus laevis choroid plexus cells.

show abstract

Prolactin and interrenal hormone balance in adult specimens of Xenopus laevis exposed to hyperosmotic stress for up to one week

et al. 1993

View full text Add to dashboard Cite

Adult female specimens of Xenopus laevis were exposed to diluted artificial seawater for up to 1 week. Statistically significant increases were observed in serum levels of aldosterone and corticosterone, concomitant with a drop in levels of prolactin (PRL) receptors in the cell membranes of the epidermis and kidney, which can be taken as an indicator of the levels of circulating PRL. The changes in hormone levels were detected after 1 day, being followed by a gradual restoration of the initial mean levels of these hormones when the exposure to hyperosmotic stress was extended for up to 2 or 7 days. Comparison of the above results with those previously obtained in Xenopus after longer exposure (15 days) to brackish water substantiates the necessity of recording possible initial fluctuations, as well as any changes that occur over the course of longer exposure during which time adaptation to environmental changes are likely to take place. The reverse responses, which may be not independent of one another, in terms of levels of corticoids and PRL in the animals exposed to hyperosmotic stress, are discussed. A survey of the literature suggests that, in Xenopus, as in the case of other less strictly water-dwelling amphibians, PRL is one of the hormones required for life in fresh water.

show abstract

Further study on the changes in the concentration of prolactin-binding sites in different organs of Xenopus laevis male and female, kept under dry conditions and then returned to water (their natural habitat)

Cited by 11 publications

References 19 publications

Localization of prolactin receptor in the dorsal and ventral skin of the frog (Rana ridibunda)

Localization of prolactin receptor in the dorsal and ventral skin of the frog (Rana ridibunda)

Biochemical study of prolactin binding sites in Xenopus laevis brain and choroid plexus

Prolactin and interrenal hormone balance in adult specimens of Xenopus laevis exposed to hyperosmotic stress for up to one week

Contact Info

Product

Resources

About