Fusaristatin A production negatively affects the growth and aggressiveness of the wheat pathogen Fusarium pseudograminearum

Khudhair, Mohammed W.; Kazan, Kemal; Thatcher, Louise F.; Obanor, Friday; Rusu, Anca; Sørensen, Jens Laurids; Wollenberg, Rasmus Dam; McKay, Alan; Giblot‐Ducray, D.; Simpfendorfer, S.; Aitken, E. A. B.; Gardiner, Donald M.

doi:10.1016/j.fgb.2019.103314

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…The FGSG_08210, a hypothetical protein, is known to be a member of the PKS6 gene cluster required for the biosynthesis of the lipopepdie fusaristatin A in F. graminearum ( Sørensen et al, 2014 ). Fusaristatin A is known to have a negative effect on fungal growth and aggressiveness in the wheat pathogen F. pseudograminearum ( Khudhair et al, 2020 ). Although it is unknown if FGSG_08210 is essential for fusaristatin A production in F. graminearum , it is likely that fusaristatin A biosynthetic pathway is associated with the maintenance of natural hyphal collapse.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum

Choi,

Kim,

Yun

2024

Plant Pathol J

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Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.

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Section: Discussionmentioning

confidence: 99%

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum

Choi,

Kim,

Yun

2024

Plant Pathol J

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“…Natural populations of Fusarium pseudograminearum having similar deletion (PKS6 missing and NRPS7 truncated) in this biosynthetic gene cluster exist in western Australia and are more aggressive pathogens causing crown rot of wheat ( Wollenberg et al . 2019 ; Khudhair et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…2020 ). The experimental deletion of PKS6 and the disruption of NRPS7 in two isolates of F. pseudograminearum increased the growth rate and aggressiveness of the mutants toward wheat ( Khudhair et al . 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The landscape and predicted roles of structural variants in Fusarium graminearum genomes

Dhakal,

Kim,

Toomajian

2024

G3: Genes, Genomes, Genetics

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Structural rearrangements, such as inversions, translocations, duplications, and large insertions and deletions, are large-scale genomic variants that can play an important role in shaping phenotypic variation and in genome adaptation and evolution. We used chromosomal-level assemblies from eight Fusarium graminearum isolates to study structural variants and their role in fungal evolution. We generated the assemblies of four of these genomes after Oxford Nanopore sequencing. A total of 87 inversions, 159 translocations, 245 duplications, 58,489 insertions and 34,102 deletions were detected. Regions of high recombination rate are associated with structural rearrangements, and a significant proportion of inversions, translocations, and duplications overlap with the repeat content of the genome, suggesting recombination and repeat elements are major factors in the origin of structural rearrangements in F. graminearum. Large insertions and deletions introduce presence-absence polymorphisms for many genes, including secondary metabolite biosynthesis cluster genes and predicted effectors genes. Translocation events were found to be shuffling predicted effector-rich regions of the genomes and are likely contributing to the gain and loss of effectors facilitated by recombination. Breakpoints of some structural rearrangements fall within coding sequences and are likely altering the protein products. Structural rearrangements in F. graminearum thus have an important role to play in shaping pathogen-host interactions and broader evolution through genome reorganization, the introduction of presence-absence polymorphisms, and changing protein products and gene regulation.

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“…Xanthocillins, encoded by an isocyanide gene cluster, participate in copper homeostasis ( Raffa et al, 2021 ). Some fungal secondary metabolites are required for asexual spore development ( Zhang et al, 2017 ) and some even suppress fungal growth such as fusaristatin A produced by certain isolates of Fusarium pseudograminearum ( Khudhair et al, 2020 ). Additionally, the literature is rich with examples of how fungal natural products provide protection/secure environmental niches when utilized as “weapons” against other microbes ( Keller, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans

Palmer

Wiemann

Greco

et al. 2021

Journal of Industrial Microbiology and Biotechnology

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Many fungi develop both asexual and sexual spores that serve as propagules for dissemination and/or recombination of genetic traits. Asexual spores are often heavily pigmented and this pigmentation provides protection from UV light. However, little is known about any purpose pigmentation that may serve for sexual spores. The model Ascomycete Aspergillus nidulans produces both green pigmented asexual spores (conidia) and red pigmented sexual spores (ascospores). Here we find that the previously characterized red pigment, asperthecin, is the A. nidulans ascospore pigment. The asperthecin biosynthetic gene cluster is composed of three genes: aptA, aptB, and aptC, where deletion of either aptA (encoding a polyketide synthase) or aptB (encoding a thioesterase) yields small, mishappen hyaline ascospores; while deletion of aptC (encoding a monooxygenase) yields morphologically normal but purple ascospores. ∆aptA and ∆aptB but not ∆aptC or wild type ascospores are extremely sensitive to UV light. We find that two historical ascospore color mutants, clA6 and clB1, possess mutations in aptA and aptB sequences, respectively.

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Fusaristatin A production negatively affects the growth and aggressiveness of the wheat pathogen Fusarium pseudograminearum

Cited by 10 publications

References 34 publications

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum

The landscape and predicted roles of structural variants in Fusarium graminearum genomes

The sexual spore pigment asperthecin is required for normal ascospore production and protection from UV light in Aspergillus nidulans

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