Fusarium graminearum as a producer of xylanases with low cellulases when grown on wheat bran

Cruz-Davila, Jhon; Perez, Jeffrey D. Vargas; Sosa, Daynet; Dı́ez, Nardy

doi:10.1016/j.btre.2022.e00738

Cited by 15 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Xylanase produced by F. graminearum FGSG_03624 activates the immune response of plants and enhances their resistance to bacterial and fungal pathogens [ 190 ]. In addition, F. graminearum Ec220 produces a xylanase with low cellulase content when grown on wheat bran [ 191 ], which has good commercial prospects. FOV PR-33 produces fusarinolic acid and its isomers, dehydrofusaric acid and fusaric acid, which are resistant to pathogenic bacteria and yeasts; these metabolites are potential antibacterial drugs [ 192 ].…”

Section: Discussionmentioning

confidence: 99%

Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Man

Zhu

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

Cotton is an important economic crop. Fusarium and Verticillium are the primary pathogenic fungi that threaten both the quality and sustainable production of cotton. As an opportunistic pathogen, Fusarium causes various human diseases, including fungal keratitis, which is the most common. Therefore, there is an urgent need to study and clarify the resistance mechanisms of cotton and humans toward Fusarium in order to mitigate, or eliminate, its harm. Herein, we first discuss the resistance and susceptibility mechanisms of cotton to Fusarium and Verticillium wilt and classify associated genes based on their functions. We then outline the characteristics and pathogenicity of Fusarium and describe the multiple roles of human neutrophils in limiting hyphal growth. Finally, we comprehensively compare the similarities and differences between animal and plant resistance to Fusarium and put forward new insights into novel strategies for cotton disease resistance breeding and treatment of Fusarium infection in humans.

show abstract

Section: Discussionmentioning

confidence: 99%

Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Man

Zhu

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Hasil pengujian didapatkan ketiga sampel positif memiliki zona bening (halo) yang terlihat disekitar koloni (Gambar 4). Dalam penelitian lainnya disebutkan bahwa Fusarium graminearum (Davilla et al, 2022, Kikot et al, 2010 dan Fusarium oxysporum (Onofre, 2013), diketahui dapat menghasilkan enzim selulase, sedangkan Colletotrichum coccodes belum pernah dilaporkan menghasilkan enzim selulase.…”

Section: Seleksi Fungi Endofit Penghasil Selulaseunclassified

“…Fusarium graminearum diketahui dapat menghasilkan enzim selulase hingga 0,243 U/ml pada penelitian Davilla et al (2022). Pada penelitian Fusarium graminearum yang diisolasi dari wilayah kaya akan gandum dapat menghasilkan aktivitas selulolitik hingga 320-330 mU/mL pada hari ketujuh hingga kesembilan (Kikot et al, 2010).…”

Section: Uji Aktivitas Selulolitik Dengan Metode Kuantitatifunclassified

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Dhia Salsabila Hakim,

Kasiamdari

2023

bib

View full text Add to dashboard Cite

Abstrak: Fungi endofit menjadi perhatian di industri bioetanol karena peranannya dalam sekresi metabolit sekunder berupa enzim selulolitik yang diketahui lebih besar daripada bakteri. Sekresi enzim metabolik pada fungi endofit tergantung pada inang yang ditempatinya. Tanaman herbal dikenal sebagai tempat hidup berbagai fungi endofit. Daun sirih hijau (Piper betle L.) merupakan salah satu tanaman herbal yang dikenal memiliki kandungan metabolit tinggi. Penelitian ini bertujuan untuk mengidentifikasi fungi endofit yang hidup pada P. betle, melakukan uji selulolitik fungi hasil identifikasi serta mengetahui fungi endofit yang memiliki aktivitas selulolitik tebesar. Uji selulolitik dilakukan dengan dua tahap yaitu seleksi dengan Congo Red serta aktivitas selulolitik dihitung dengan kadar gula reduksi. Hasil penelitian didapatkan tiga spesies fungi dari P. betle yaitu Fusarium graminearum, Fusarium oxysporum dan Colletotrichum coccodes. Ketiga sampel yang diujikan menunjukkan positif menghasilkan enzim selulase yang ditandai dengan adanya zona bening di sekitar koloni. Fungi endofit penghasil enzim selulase terbesar adalah spesies F. oxysporum dengan angka 0,033 U/ml.

show abstract

“…For example, Javed et al ( 8 ) investigated the possibility of xylanase production in agricultural waste such as wheat bran using the fungus A. niger KIBGE-IB36. The xylanolytic potential of the endophytic fungus F. graminearum isolated from leaves of Theobroma cacao was investigated for the first time for the production of xylanase (low cellulase contents) when grown on wheat bran as the sole carbon source ( 9 ). A. awamori AFE1 and cassava peel were used for the production of thermally stable, acidophilic and surfactant-tolerant xylanase ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Šekuljica,

Jakovetić Tanasković,

Mijalković

et al. 2023

Food Technol. Biotechnol. (Online)

View full text Add to dashboard Cite

Research background. The development of a novel process for the production of xylooligosaccharides based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification, together with the physical pretreatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, xylooligosaccharides. Experimental approach. To produce xylanase, a solid-state fermentation was performed using wheat bran as substrate. In order to obtain the most active xylanase crude extract, the time frame of the cultivation process was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterization included determination of the optimal pH and temperature, determination of the molecular mass of the purified xylanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the partially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a 49 % yield and 13-fold xylanase purification was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/(min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyze the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chromatography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerization was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focused on creating new enzymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.

show abstract

Fusarium graminearum as a producer of xylanases with low cellulases when grown on wheat bran

Cited by 15 publications

References 52 publications

Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans

Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase

Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls

Contact Info

Product

Resources

About