Fusarium oxysporum- und F. proliferatum-Isolate aus Spargel und deren Pathogenitätsüberprüfung in einer modifizierten in vitro-Schnelltestmethode

Goßmann, M.; Scholz, A.; Hennig, Frank; Bargen, Susanne von; Büttner, Carmen

doi:10.1007/s10343-011-0262-x

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…against F. oxysporum and recommended it as a rapid resistance screening technique. Gossmann et al (2011) [21] tested the pathogenicity of Fusarium spp. on young seedlings that grew in water agar in big test tubes filled with water agar.…”

Section: Discussionmentioning

confidence: 99%

Root Growth and Defense Response of Seedlings against Fusarium oxysporum in Sand Culture and In Vitro—A Comparison of Two Screening Approaches for Asparagus Cultivars

2023

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Two rapid asparagus (Asparagus officinalis L.) screening methods, in sand culture and in vitro, were tested to evaluate the response of young seedlings against F. oxysporum f. sp. asparagi (isolate Foa1). Root morphological parameters were evaluated and correlated with the symptomatology and expression of the defense-related genes at 5 and 7 dpi. In sand cultivation, the Foa1-inoculated cultivars showed no visible disease symptoms on their roots until 7 dpi. Two-factorial ANOVA statistics found no significant interaction between the cultivars and treatments for most root parameters but some differences between the cultivars. The in vitro Foa1-inoculated cultivars showed high susceptibility according to their symptomatology and differed greatly in the length of the primary root at 5 dpi. In some cultivars, the primary root length and root surface area were higher upon Foa1 inoculation. The expression changes were very different among the cultivars, with significant induction of PR1, POX, and PAL at 5 dpi in all cultivars in vitro but only in two cultivars in sand cultivation. The in vitro screening method, although more artificial, seemed to be more reliable than sand cultivation since the fungus was able to develop well in the culture medium. In sand-filled pots, the fungus may have been hindered in its development, even though a considerable higher amount of Foa1 was inoculated. In addition, the fungal growth was easily trackable in tubes, while in sand cultivation, the results were only visible after pulling the seedlings out of the pots 12 dpi.

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Section: Discussionmentioning

confidence: 99%

Root Growth and Defense Response of Seedlings against Fusarium oxysporum in Sand Culture and In Vitro—A Comparison of Two Screening Approaches for Asparagus Cultivars

2023

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“…cannot be efficiently controlled by plant protection or cropping procedures. Therefore, breeding of cultivars resistant to F. oxysporum infection could be the most successful and economically reasonable strategy for controlling crown and root rot (Corpas‐Hervias, Melero‐Vara, Molinero‐Ruiz, Zurera‐Muñoz, & Basallote Ureba, ; Goßmann, Scholz, Hennig, Bargen, & Büttner, ; Stephens, Vries, & Sink, ).…”

Section: Introductionmentioning

confidence: 99%

“…A non‐destructive method like a bioassay offers the possibility of efficient trait detection as well as selection of individual plants with desirable features. The most common approach in numerous studies is the visual symptom scoring of infested plant tissues based on assessment keys (Apaza, Talledo, & Casas, ; Blok & Bollen ; Elmer et al, ; Goßmann et al, ). The aim of the present study was to develop a bioassay for screening juvenile asparagus plants for resistance to F. oxysporum applicable in research and breeding.…”

Section: Introductionmentioning

confidence: 99%

Development of a bioassay to assess resistance to Fusarium oxysporum (Schlecht.) in asparagus (Asparagus officinalis L.)

Kathe

Krämer

Budahn

et al. 2019

Journal of Phytopathology

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Fusarium oxysporum is one of the major pathogens causing root and crown rot in asparagus. Breeding of cultivars resistant to F. oxysporum would be the most efficient strategy for pathogen control. In this study, a bioassay was developed for screening seedling resistance. The non‐destructive bioassay comprises inoculation with a highly aggressive F. oxysporum isolate, incubation in a climate chamber and quantification of disease symptoms by a digital image analysing system and a PTA‐ELISA. This bioassay is simple to implement and demonstrated high reproducibility. Subsequently, it was used to determine the resistance behaviour of 16 asparagus genotypes to F. oxysporum. The asparagus cultivars revealed different levels of susceptibility, whereas the wild relative A. densiflorus was confirmed to be resistant.

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Effect of plastic mulching on mycotoxin occurrence and mycobiome abundance in soil samples from asparagus crops

et al. 2015

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Plastic mulching (PM) is widely used in modern agriculture because of its advantageous effects on soil temperature and water conservation, factors which strongly influence the microbiology of the soil. The aim of this study was to assess the effect of PM on mycotoxin occurrence in relation with mycobiome abundance/diversity and soil physicochemical properties. Soil samples were collected from green (GA) and white asparagus (WA) crops, the last under PM. Both crops were cultivated in a ridge-furrow-ridge system without irrigation. Samples were analyzed for mycotoxin occurrence via liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Total colony-forming unit was indicative of mycobiome abundance, and analysis of mycobiome diversity was performed by internal transcribed spacer (ITS) sequencing. PM avoided the drop of soil temperature in winter and allowed higher soil temperature in early spring compared to non-covered soil. Moreover, the use of PM provided controlled conditions for water content in soil. This was enough to generate a dissimilar mycotoxin occurrence and mycobiome diversity/abundance in covered and non-covered soil. Mycotoxin soil contamination was confirmed for deoxynivalenol (DON), range LOD to 32.1 ng/g (LOD = 1.1 ng/g). The DON values were higher under PM (average 16.9 ± 10.1 ng/g) than in non-covered soil (9.1 ± 7.9 ng/g); however, this difference was not statically significant (p = 0.09). Mycobiome analysis showed a fungal compartment up to fivefold higher in soil under PM compared to GA. The diversity of the mycobiome varied between crops and also along the soil column, with an important dominance of Fusarium species at the root zone in covered soils.

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Fusarium oxysporum- und F. proliferatum-Isolate aus Spargel und deren Pathogenitätsüberprüfung in einer modifizierten in vitro-Schnelltestmethode

Cited by 3 publications

References 27 publications

Root Growth and Defense Response of Seedlings against Fusarium oxysporum in Sand Culture and In Vitro—A Comparison of Two Screening Approaches for Asparagus Cultivars

Root Growth and Defense Response of Seedlings against Fusarium oxysporum in Sand Culture and In Vitro—A Comparison of Two Screening Approaches for Asparagus Cultivars

Development of a bioassay to assess resistance to Fusarium oxysporum (Schlecht.) in asparagus (Asparagus officinalis L.)

Effect of plastic mulching on mycotoxin occurrence and mycobiome abundance in soil samples from asparagus crops

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