Agricultural activities make significant contributions to the national economies around the world. Factors such as rapid population growth and unplanned urbanization cause a decrease in agricultural lands and agricultural crop production over the years with the effect of abiotic and biotic factors. Many studies have been carried out to eliminate factors that reduce crop productivity. Fungal pathogens, one of the leading biotic factors, cause plant diseases and reduce the production efficiency of crops. This study aimed to isolate Streptomyces genus bacteria, which can be used as a biological agent against the wheat pathogen Fusarium culmorum, and to evaluate their antifungal activities. We found that eight of the 25 Streptomyces isolates, which were isolated from soil using the starch-casein agar medium and identified basis on morphological characteristics (colony appearance and microscopic examination results), inhibited the growth of F. culmorum on the Mueller-Hinton agar and that Streptomyces species could be used as a biological agent against this pathogen. We consider that eight Streptomyces isolates having different degrees of antifungal activity is promising for more permanent, economical and efficient solutions against F. culmorum, which is one of the fungi that reduces agricultural economic yield in Turkey. This study can also guide further research that will evaluate the potential use secondary metabolites of Streptomyces bacteria with antifungal properties for biocontrol or promotion of plant growth in different forms of microbial fertilizers.