Fusarium udum revisited: a common, but poorly understood member of the Fusarium fujikuroi species complex

Pfenning, Ludwig H.; Melo, Maruzanete Pereira de; Costa, Marileide Moreira; Reis, Ailton; Cabral, C. S.; Lima, Cristiano Souza; Abreu, Lucas M.; Costa, S. S.

doi:10.1007/s11557-018-1446-x

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…At the moment, it is not possible to affirm the real contribution of the sexual reproduction of F. sacchari in the field to the epidemic of the disease. The sexual stage of species in the FFSC is not commonly observed in the field and has been reported in the literature only for F. udum, F. verticillioides and F. xylarioides (Edwards, 1935;Leslie & Summerell, 2006;Pfenning et al, 2018). However, it is known that sexual recombination is important for long-term survival and adaptation of the species in the field (Leslie & Klein, 1996;Leslie & Summerell, 2006).…”

Section: Discussionmentioning

confidence: 99%

Identification and pathogenicity of Fusarium species associated with pokkah boeng of sugarcane in Brazil

et al. 2019

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Brazil is the world’s biggest producer of sugarcane (Saccharum spp. hybrids). Pokkah boeng is an important fungal disease in this crop caused by members of the Fusarium fujikuroi species complex (FFSC) and characterized by deformation of the aerial part of the plant and stem rot. While the occurrence of symptoms has been reported in plantations in Brazil, no official reports of the disease exist. In this study, species of the FFSC were identified that are associated with sugarcane plants with symptoms of pokkah boeng in Brazil. This was achieved using two‐loci molecular phylogeny, sexual compatibility and analysis of morphological markers. The ability of strains to cause disease in plants of sugarcane, maize, sorghum and millet was also evaluated. The 39 isolates studied were identified as F. sacchari, F. proliferatum and another, still unknown, phylogenetic lineage that is sister to F. andiyazi. Crossing field isolates of F. sacchari and F. proliferatum with their respective tester strains produced fertile perithecia and viable ascospores. All three species induced symptoms of pokkah boeng on inoculated sugarcane plants and caused stem rot in maize, sorghum and millet. Symptoms on sugarcane are chlorosis and necrosis of leaves, punctured lesions, twisted leaves, reduction of the total leaf area, death of the top of the plant and stalk rot. The findings confirmed the aetiology of the disease in Brazil, generated basic knowledge for the development of strategies for diagnosis and monitoring of the disease and support breeding programmes for selecting resistant germplasm.

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Section: Discussionmentioning

confidence: 99%

Identification and pathogenicity of Fusarium species associated with pokkah boeng of sugarcane in Brazil

et al. 2019

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“…Descriptions and illustrations : See Wollenweber & Reinking (1935) , Booth (1971) , Subramanian (1971) , Booth (1978) , Gerlach & Nirenberg (1982) and Pfenning et al. (2019) .…”

Section: Fusarium and Allied Genera: List Of Accepted Namesmentioning

confidence: 99%

Fusarium: more than a node or a foot-shaped basal cell

Crous¹,

Lombard²,

Sandoval-Denis³

et al. 2021

Studies in Mycology

205

133

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Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae . Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae . Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris ). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium . Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae . Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae ( e.g. , Cosmosporella , Macroconia , Microcera ). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium . To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org . The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa ( ...

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“…Fusarium udum causes a wilt disease of Cajanus cajan and Crotalaria spp. in tropical regions ( Pfenning et al . 2019 ), and is reported here from Sorghum field soil in Ethiopia.…”

Section: Discussionmentioning

confidence: 99%

Fusarium diversity associated with the Sorghum-Striga interaction in Ethiopia

Lombard

Doorn

Groenewald

et al. 2022

Fungal Systematics and Evolution

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Sorghum production is seriously threatened by the root parasitic weeds (RPWs) Striga hermonthica and Striga asiatica in sub-Saharan Africa. Research has shown that Striga control depends on eliminating its seed reserves in soil. Several species of the genus Fusarium (Nectriaceae, Hypocreales) , which have been isolated from diseased Striga plants have proven to be highly pathogenic to all developmental stages of these RPWs. In the present study 439 isolates of Fusarium spp. were found associated with soils from Sorghum growing fields, Sorghum rhizosphere, or as endophytes with Sorghum roots and seeds, or as endophytes of Striga stems and seeds. Based on multi-locus phylogenies of combinations of CaM, tef1, rpb1 and rpb2 alignments, and morphological characteristics, 42 species were identified, including three species that are newly described, namely F. extenuatum and F. tangerinum from Sorghum soils, and F. pentaseptatum from seed of Striga hermonthica. Using a previously published AFLP-derived marker that is specific to detect isolates of F. oxysporum f. sp. strigae, an effective soil-borne biocontrol agent against Striga, we also detected the gene in several other Fusarium species. As these isolates were all associated with the Striga/Sorghum pathosystem, the possibility of horizontal gene transfer among these fusaria will be of interest to further investigate in future.

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Fusarium udum revisited: a common, but poorly understood member of the Fusarium fujikuroi species complex

Cited by 10 publications

References 19 publications

Identification and pathogenicity of Fusarium species associated with pokkah boeng of sugarcane in Brazil

Identification and pathogenicity of Fusarium species associated with pokkah boeng of sugarcane in Brazil

Fusarium: more than a node or a foot-shaped basal cell

Fusarium diversity associated with the Sorghum-Striga interaction in Ethiopia

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