Fusicatenibacter saccharivorans gen. nov., sp. nov., isolated from human faeces

Abstract

Three Gram-stain-positive, obligately anaerobic, non-motile, non-spore-forming, spindle-shaped bacterial strains (HT03-11T, KO-38 and TT-111), isolated from human faeces were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing showed that the strains were highly related to each other genetically (displaying >99 % sequence similarity) and represented a previously unknown subline within the Blautia coccoides rRNA group of organisms (cluster XIVa). The closest phylogenetic neighbours of strain HT03-11T were Clostridium bolteae WAL 16351T (93.7 % 16S rRNA gene sequence similarity) and Clostridium saccharolyticum WM1T (93.7 % similarity). All isolates produced lactic acid, formic acid, acetic acid and succinic acid as fermentation end products from glucose. Their chemotaxonomic properties included lysine as the cell wall diamino acid and C16 : 0, C18 : 1ω7c DMA and C16 : 0 DMA as the major fatty acids. The G+C contents of the genomic DNA were 46.9–47.2 mol% (HPLC). Several phenotypic and chemotaxonomic characteristics could be readily used to differentiate the isolates from phylogenetically related clostridia. Therefore, strains HT03-11T, KO-38 and TT-111 represent a novel species in a new genus of the family Lachnospiraceae , for which the name Fusicatenibacter saccharivorans gen. nov., sp. nov. is proposed. The type strain of the type species is HT03-11T ( = YIT 12554T = JCM 18507T = DSM 26062T).

Diversity of Intestinal Clostridium coccoides Group in the Japanese Population, as Demonstrated by Reverse Transcription-Quantitative PCR

“…A large number of species of genera such as Clostridium , Blautia , Dorea , Eubacterium , Ruminococcus , Anaerostipes , Roseburia , and Coprococcus belong to the group [ 2 , 5 ]. A clone library analysis has shown that not only culturable species but also many as-yet-uncultured bacteria are included in this group [ 6 ]: our team has recently identified a new species, Fusicatenibacter saccharivorans , in the group [ 7 ].…”

Gut metabolites and bacterial community networks during a pilot intervention study with flaxseeds in healthy adult men

“…The fact that different members of one family may be associated with different steps of activation of dietary lignans could explain the discrepancy between previous findings on correlations between enterolignan production and bacterial composition as measured using 16S probes that target entire phylogenetic groups . The impact of the newly described species Fusicatenibacter saccharivorans on lignan metabolism in the gut may also be worth investigating, since one corresponding OTU was found in most of the samples at a mean abundance >2% and correlated positively with blood concentrations of matairesinol . Despite the reported ability of bifidobacteria to deglycosylate SDG and the fact that flaxseeds contain a substantial amount of indigestible carbohydrates, we did not observe a bifidogenic effect associated with flaxseed ingestion , most likely because many other bacteria in the gut can compete for these substrates .…”

Cultivation-based multiplex phenotyping of human gut microbiota allows targeted recovery of previously uncultured bacteria

“…To identify optimal anaerobic cultivation conditions for cultivating the human gut microbiota, we tested the growth of bacteria from faecal samples on 10 different solid media. These media were chosen to represent a range of nutrient concentrations and several had previously been shown to have good potential for recovery of gut bacteria 22,24,25 . The goal was to try to more closely represent the conditions in the gut; hence, a careful consideration of media components was undertaken (Supplementary Discussion).…”