Fusicoccin-lnduced Growth and Dark Respiration in Rye Coleoptiles

Kutschera, U.

doi:10.1016/s0176-1617(99)80297-1

Cited by 4 publications

(7 citation statements)

References 12 publications

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“…These authors measured a slight (5 ± 10%) auxin-induced stimulation in the rate of dark respiration. Corresponding experiments with rye coleoptile segments revealed no positive IAA effect on oxygen uptake (Kutschera, 1999). The results reported here (Fig.…”

Section: Discussionsupporting

confidence: 74%

“…The rate of dark respiration (oxygen uptake) was measured polarographically using a Clark-type oxygen electrode (Kutschera, 1999). Between days 2 and 7 after sowing, 10 segments (10 mm in length) were excised from the coleoptiles (see Fig.…”

Section: Dark Respiration and Electron Microscopymentioning

confidence: 99%

“…The samples (10 segments each) were placed into the measurement vessel of the oxygen electrode that contained 8 ml of distilled water (100 % air saturation). The rate of O 2 uptake was determined as described previously (Kutschera, 1999).…”

Section: Dark Respiration and Electron Microscopymentioning

confidence: 99%

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The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹

Kutschera

2004

Plant Biology

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The relationships between changes in irreversible and reversible organ length, turgor (P), osmotic pressure (pi), and metabolic activity of the cells were investigated in intact coleoptiles of rye seedlings ( Secale cereale L.) that were either grown in darkness or irradiated with continuous white light. Cessation of growth at day 4 after sowing was associated with an apparent mechanical stiffening of the cell walls. Turgor pressure was measured in epidermal and mesophyll cells with a miniaturized pressure probe. No gradient of turgor was found between the peripheral and internal cells. In juvenile (growing) coleoptiles, average turgor was 0.60 MPa and a negative water potential (P - pi) was established in these cells. Upon emergence of the primary leaf, turgor declined, but P was maintained at values of 0.43 and 0.52 MPa in 7-day-old light- and dark-grown coleoptiles, respectively. Water potential in non-growing cells approached zero. The rate of dark respiration and elongation growth were not correlated. Surgical removal of the mature coleoptile revealed that the erect position of the 7-day-old shoot was dependent on the presence of this sturdy, turgid organ sheath. It is concluded that, during the first week of seedling development, the pierced, metabolically active coleoptile fulfills an essential function as an elastic basal tube for the juvenile shoot.

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Section: Discussionsupporting

confidence: 74%

Section: Dark Respiration and Electron Microscopymentioning

confidence: 99%

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The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹

Kutschera

2004

Plant Biology

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“…These values were subtracted and the rates of oxygen uptake (i.e., cellular respiration) calculated. 15 The fresh and dry mass (M f and M d , respectively) of embryos and entire seedlings, taken from the same batch of plants, were (i.e., R ~ M -1.0 ; α RMA ~ 1.0) reported by Reich et al 9 based on an interspecific analysis of data collected from ca. 500 laboratory and field-grown seedlings and tree saplings.…”

Section: Resultsmentioning

confidence: 89%

“…1A and B) differ markedly with respect to their metabolic scaling darkness, R) was measured polarographically using a Clarktype oxygen electrode as described by Kutschera. 15 The rate of O 2 -depletion of air-saturated water was recorded over a period of 20 min in darkness (25°C). Thereafter, the O 2 -uptake of killed (boiled-cooled down) samples was determined as a control (i.e., physical O 2 absorption of the tissues unrelated to metabolic activity of the cells).…”

Section: Resultsmentioning

confidence: 99%

Ontogenetic changes in the scaling of cellular respiration with respect to size among sunflower seedlings

Kutschera

Niklas

2011

Plant Signaling & Behavior

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Organ-specific rates of cellular respiration in developing sunflower seedlings and their bearing on metabolic scaling theory

Kutschera

Niklas

2011

Protoplasma

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Fifty years ago Max Kleiber described what has become known as the "mouse-to-elephant" curve, i.e., a log-log plot of basal metabolic rate versus body mass. From these data, "Kleiber's 3/4 law" was deduced, which states that metabolic activity scales as the three fourths-power of body mass. However, for reasons unknown so far, no such "universal scaling law" has been discovered for land plants (embryophytes). Here, we report that the metabolic rates of four different organs (cotyledons, cotyledonary hook, hypocotyl, and roots) of developing sunflower (Helianthus annuus L.) seedlings grown in darkness (skotomorphogenesis) and in white light (photomorphogenesis) differ by a factor of 2 to 5 and are largely independent of light treatment. The organ-specific respiration rate (oxygen uptake per minute per gram of fresh mass) of the apical hook, which is composed of cells with densely packaged cytoplasm, is much higher than that of the hypocotyl, an organ that contains vacuolated cells. Data for cell length, cell density, and DNA content reveal that (1) hook opening in white light is caused by a stimulation of cell elongation on the inside of the curved organ, (2) respiration, cell density and DNA content are much higher in the hook than in the stem, and (3) organ-specific respiration rates and the DNA contents of tissues are statistically correlated. We conclude that, due to the heterogeneity of the plant body caused by the vacuolization of the cells, Kleiber's law, which was deduced using mammals as a model system, cannot be applied to embryophytes. In plants, this rule may reflect scaling phenomena at the level of the metabolically active protoplasmic contents of the cells.

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Fusicoccin-lnduced Growth and Dark Respiration in Rye Coleoptiles

Cited by 4 publications

References 12 publications

The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹

The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹

Ontogenetic changes in the scaling of cellular respiration with respect to size among sunflower seedlings

Organ-specific rates of cellular respiration in developing sunflower seedlings and their bearing on metabolic scaling theory

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Fusicoccin-lnduced Growth and Dark Respiration in Rye Coleoptiles

Cited by 4 publications

References 12 publications

The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development1

The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development1

Ontogenetic changes in the scaling of cellular respiration with respect to size among sunflower seedlings

Organ-specific rates of cellular respiration in developing sunflower seedlings and their bearing on metabolic scaling theory

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The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹

The Biophysical Basis of Cell Elongation and Organ Maturation in Coleoptiles of Rye Seedlings: Implications for Shoot Development¹