Fusion of a fission yeast

Davey, John

doi:10.1002/(sici)1097-0061(199812)14:16<1529::aid-yea357>3.0.co;2-0

Cited by 95 publications

(84 citation statements)

References 321 publications

(488 reference statements)

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“…Under these conditions, haploid cells of the opposite mating types can conjugate to form a diploid zygote, which rapidly undergoes meiosis and sporulation and produces an ascus (reviewed in Ref. 26). Under either nitrogen or carbon starvation, ϳ60% of wild type cells underwent mating compared with less than 1% of ⌬tor1 cells.…”

Section: Identification Of Tor Homologs In the S Pombe Genome-mentioning

confidence: 99%

“…26). Under these conditions most of the wild type diploid cells, Ͼ60%, underwent sporulation, whereas Ͻ1% of homozygous ⌬tor1 diploids sporulated (results not shown).…”

Section: Identification Of Tor Homologs In the S Pombe Genome-mentioning

confidence: 99%

“…Upon starvation, S. pombe cells enter either the stationary phase or the sexual development pathway (reviewed in Ref. 26). We previously reported that rapamycin has a different effect on S. pombe compared with its effect on S. cerevisiae.…”

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confidence: 99%

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The Fission Yeast TOR Homolog,tor1 +, Is Required for the Response to Starvation and Other Stresses via a Conserved Serine

Weisman

Choder

2001

Journal of Biological Chemistry

181

192

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Targets of rapamycin (TORs) are conserved phosphatidylinositol kinase-related kinases that are involved in the coordination between nutritional or mitogenic signals and cell growth. Here we report the initial characterization of two Schizosaccharomyces pombe TOR homologs, tor1 ؉ and tor2 ؉ . tor2 ؉ is an essential gene, whereas tor1؉ is required only under starvation and other stress conditions. Specifically, ⌬tor1 cells fail to enter stationary phase or undergo sexual development and are sensitive to cold, osmotic stress, and oxidative stress. In complex with the prolyl isomerase FKBP12, the drug rapamycin binds a conserved domain in TORs, FRB, thus inhibiting some of the functions of TORs. Mutations at a conserved serine within the FRB domain of Saccharomyces cerevisiae TOR proteins led to rapamycin resistance but did not otherwise affect the functions of the proteins. The S. pombe tor1 ؉ exhibits different features; substitution of the conserved serine residue, Ser 1834 , with arginine compromises its functions and has no effect on the inhibition that rapamycin exerts on sexual development in S. pombe.

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Section: Identification Of Tor Homologs In the S Pombe Genome-mentioning

confidence: 99%

“…26). Under these conditions most of the wild type diploid cells, Ͼ60%, underwent sporulation, whereas Ͻ1% of homozygous ⌬tor1 diploids sporulated (results not shown).…”

Section: Identification Of Tor Homologs In the S Pombe Genome-mentioning

confidence: 99%

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The Fission Yeast TOR Homolog,tor1 +, Is Required for the Response to Starvation and Other Stresses via a Conserved Serine

Weisman

Choder

2001

Journal of Biological Chemistry

181

192

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“…When nitrogen becomes scarce the cAMP level decreases, which triggers the expression of genes required for sexual differentiation. Cells arrest in G1 and polarize, giving rise to specialized cells termed shmoos (Yamamoto et al 1997;Davey 1998;Nielsen 2004;Yamamoto 2004). After agglutination, the cross wall separating both parental cells is degraded, allowing cell fusion.…”

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confidence: 99%

Membrane Organization and Cell Fusion During Mating in Fission Yeast Requires Multipass Membrane Protein Prm1

et al. 2014

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The involvement of Schizosaccharomyces pombe prm1 + in cell fusion during mating and its relationship with other genes required for this process have been addressed. S. pombe prm1D mutant exhibits an almost complete blockade in cell fusion and an abnormal distribution of the plasma membrane and cell wall in the area of cell-cell interaction. The distribution of cellular envelopes is similar to that described for mutants devoid of the Fig1-related claudin-like Dni proteins; however, prm1 + and the dni + genes act in different subpathways. Time-lapse analyses show that in the wild-type S. pombe strain, the distribution of phosphatidylserine in the cytoplasmic leaflet of the plasma membrane undergoes some modification before an opening is observed in the cross wall at the cellcell contact region. In the prm1D mutant, this membrane modification does not take place, and the cross wall between the mating partners is not extensively degraded; plasma membrane forms invaginations and fingers that sometimes collapse/retract and that are sometimes strengthened by the synthesis of cell-wall material. Neither prm1D nor prm1D dniD zygotes lyse after cell-cell contact in medium containing and lacking calcium. Response to drugs that inhibit lipid synthesis or interfere with lipids is different in wild-type, prm1D, and dni1D strains, suggesting that membrane structure/organization/dynamics is different in all these strains and that Prm1p and the Dni proteins exert some functions required to guarantee correct membrane organization that are critical for cell fusion. M EMBRANE fusion is essential for several developmental processes. The characterization of mating in yeasts represents a useful tool for understanding the mechanisms involved in intercellular membrane merger and their regulation. In the fission yeast Schizosaccharomyces pombe, heterothallic cells belong to one of two mating types, M (h 2 cells) or P (h + cells) while h 90 strains are homothallic (Arcangioli and Thon 2004). In rich medium, h + and h 2 cells can proliferate actively together without mating. When nitrogen becomes scarce the cAMP level decreases, which triggers the expression of genes required for sexual differentiation. Cells arrest in G1 and polarize, giving rise to specialized cells termed shmoos (Yamamoto et al. 1997;Davey 1998;Nielsen 2004;Yamamoto 2004). After agglutination, the cross wall separating both parental cells is degraded, allowing cell fusion. Efficient cell fusion requires the correct organization of the cytoskeleton (Petersen et al. 1995(Petersen et al. , 1998aKurahashi et al. 2002;Doyle et al. 2009). The S. cerevisiae FUS1 and the S. pombe fus1 + genes have the same name and both mutants exhibit cell fusion defects during mating, leading to an accumulation of prezygotes (mating intermediates in which the mating partners have established a stable contact but have not yet fused). However, the corresponding proteins are not related; while Saccharomyces cerevisiae Fus1p is a membrane protein (Trueheart et al. 1987), S. pombe Fus1p ...

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“…Ste11 encodes for a DNA-binding protein which contains a high-mobility group (HMG) motif that binds to the TR box in mating-type specific genes (8,10). The mating-type loci, in turn, control the production of pheromones and their receptors (11). A zinc-finger protein, referred to as Rst2, directly regulates the transcription of the ste11 gene by binding to the STREP motif (12).…”

Section: Introductionmentioning

confidence: 99%

A pheromone mutant of Schizosaccharomyces pombe displays nucleolar fragmentation

Jun

Kim

2008

BMB Reports

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Stresses and nutritional starvation are two main external signals for the induction of sex pheromones in the fission yeast Schizosaccharomyces pombe. In an attempt to identify the components involved in transduction of starvation signals, we screened 135 temperature-sensitive (ts) mutants and isolated 6 mutants that induced the pheromone even in the presence of a nitrogen source. These mutants exhibited two distict induction phenotypes: pheromone induction at restrictive but not at permissive temperatures; and pheromone induction at both permissive and restrictive temperatures. The times required for the maximum pheromone induction at the restrictive temperature differed slightly in each mutant. In addition to the pheromone induction phenotype, the ts243 and ts304 mutants exhibited cell-division-cycle defects. The ts304 mutant cells showed an abnormal cytoplasmic DAPI staining pattern. The nucleolus of this mutant seemed to be fragmented, a phenomenon which is typically observed in aged yeast cells. The result of our genetic analysis indicated that the pheromone induction mutants belonged to 6 separate complementation groups. We designated these mutants pws1 to pws6. [BMB reports 2008; 41(3): 248-253]

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Fusion of a fission yeast

Cited by 95 publications

References 321 publications

The Fission Yeast TOR Homolog,tor1 +, Is Required for the Response to Starvation and Other Stresses via a Conserved Serine

The Fission Yeast TOR Homolog,tor1 +, Is Required for the Response to Starvation and Other Stresses via a Conserved Serine

Membrane Organization and Cell Fusion During Mating in Fission Yeast Requires Multipass Membrane Protein Prm1

A pheromone mutant of Schizosaccharomyces pombe displays nucleolar fragmentation

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