Fusion of the palatine shelves with heterotypic explants in the mouse

Vargas, Victor Ignacio Cruz

doi:10.1016/0003-9969(68)90108-8

Cited by 11 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anderson & Matthiessen (1967) assumed that the macrophages, because of their close proximity to the epithelial seam, contributed to its degeneration. Vargas (1968) believed that the macrophages were concerned with the removal of degenerating and dead cells, since they ordinarily do not destroy healthy cells. Others have proposed that macrophages are responsible only for the phagocytosis of cellular debris (Mato et al 1967 a;Brusati, 1969;Hayward, 1969).…”

Section: Localization Ofacidphosphatasementioning

confidence: 99%

Light microscopic and histochemical observations on the development of the palate in the Golden Syrian hamster

Ravindra¹,

Chaudhry²

1975

Plastic and Reconstructive Surgery

View full text Add to dashboard Cite

Section: Localization Ofacidphosphatasementioning

confidence: 99%

Light microscopic and histochemical observations on the development of the palate in the Golden Syrian hamster

Ravindra¹,

Chaudhry²

1975

Plastic and Reconstructive Surgery

View full text Add to dashboard Cite

“…Detailed descriptions of the in-vitro fusion of palatal shelves have been published (POURTOIS, 1966;POURTOIS, 1968 ;MYERS, PETRAKIS and LEE, 1968). Further, the fusion of palatal shelves with eyelids (VARGAS, 1968) has been reported. In a previous series of experiments the present investigators studied the in-vitro capability of several oral tissues to fuse.…”

Section: Introductionmentioning

confidence: 99%

Fusion capability of rat embryonic extra-oral tissue in vitro

Bodner¹,

Goss²,

Avery³

1970

Archives of Oral Biology

View full text Add to dashboard Cite

Sammary-Epithelial mesenchymal interaction in the fusion process was studied by the means of fusing different extremities of a 15 day 16 hr rat foetus. The extremities used were the hands, feet, and tails, which at this stage of embryologic development appear to have a similar type of epithelial covering but diier markedly in the degree of specialization of the underlag mesenchyme. Two hands from one embryo were placed in palm to palm contact and grown under organ culture conditions for 72 hr with ten pairs of hands making up the experimental group. Ten pairs of feet made up the second experimental group with two feet from the same embryo being placed in sole to sole contact and grown under organ culture conditions. The third experimental group consisted of ten pairs of tails from littermate embryo's and were placed side by side and cultured for 72 hr. In all three experimental groups, fusion with epithelial breakdown occurred though to different extents. It was noted that the degree of mesenchymal penetration across the interrupted epithelial barrier was related to the extent of specialiition of the mesenchymal tissue. The greater the degree of specialization of the mesenchyme the less was the intermingling with the mesenchyme of the opposite member. The most specialized extremity the hand, also showed the lowest incidence of the fusion process progressing as far as the epithelial breakdown stage.

show abstract

“…It is unknown whether MEE differentiation occurs in cultures of isolated alligator palatal shelves or whether the migrating alligator phenotype is dependent upon shelf contact. Most investigations suggest that mammalian palatal shelf fusion is a specific event which can only occur between closely paired shelves (Smiley and Koch, 1971,1975, but some purport to show that palatal shelves can fuse with other embryonic epithelia, such as those of the tongue, tail, or limb (Vargas, 1968;Goss et al, 1970;Bodner et al, 1970;Goss, 1976b). There have been no reports on whether the palatal shelf of one vertebrate can close with that of another.…”

mentioning

confidence: 99%

Differentiation of cultured palatal shelves from alligator, chick, and mouse embryos

1984

View full text Add to dashboard Cite

Palatal shelves from embryonic alligators, chicks, and mice were explanted at various stages of development and organ cultured in either chemically defined, serumless media or the same media supplemented with 10% fetal calf serum. Shelves from each vertebrate were either cultured singly or in contact, and heterologous combinations of palatal shelves from different animals were made: chick/mouse, chick/alligator, and mouse/alligator. Epithelial differentiation (particularly that of the medial shelf edge) was assayed by vital staining, histology, and scanning electron microscopy. In vitro medial edge epithelial differentiation, and consequently the mechanisms of palatal closure, were identical to those normally seen in vivo for each species, i.e., cobble-stoned migrating epithelia in the alligator, cell death and fusion in the mouse, and keratinisation and cleft palate in the chick. Differentiation was optimal in the chemically defined, serumless media and was independent of shelf contact in all three species. No heterologous combinations of palatal shelves closed with each other: Evidently, the modes of palatal closure in mice and alligators are sufficiently different to prevent them forming a chimeric palate, whilst neither is capable of inducing closure in a cocultured chick palatal shelf. These unified defined culture conditions make possible a large number of epithelial-mesenchymal recombination studies as well as specific inhibitor, teratogenic, and hormonal investigations.

show abstract

Fusion of the palatine shelves with heterotypic explants in the mouse

Cited by 11 publications

References 8 publications

Light microscopic and histochemical observations on the development of the palate in the Golden Syrian hamster

Light microscopic and histochemical observations on the development of the palate in the Golden Syrian hamster

Fusion capability of rat embryonic extra-oral tissue in vitro

Differentiation of cultured palatal shelves from alligator, chick, and mouse embryos

Contact Info

Product

Resources

About