Future challenges for x-ray microscopy

Jacobsen, Chris

doi:10.1063/1.4937529

Cited by 18 publications

(2 citation statements)

References 67 publications

(64 reference statements)

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“…The cells adherent to the PMMA sheathe its surface from the radiation. Depending on the cell thickness, the 3D visualization of internal structures within eukaryotic cells becomes possible [24,25].…”

Section: Introductionmentioning

confidence: 99%

Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy

et al. 2020

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This work is to study the relationship between the exposure conditions and the quality of cell imaging with soft X-ray contact microscopy (SXCM). It is a crucial step in the efficient visualization of cell structures. Three different human cell lines: DU145 prostate carcinoma cells, HCC38 breast cancer cells, and Poietics mesenchymal stem cells were used to establish the optimal exposure conditions in SXCM. The image quality depended on the soft X-ray (SXR) absorbed energy and photoresist development conditions. At lower SXR energy (200 or 400 SXR pulses), sharp cell edges, membrane projections, and cell–cell connections were visible. In contrast, higher energy (600 or 800 SXR pulses) allowed observation of the cytoskeleton and the nucleus in a cell type-dependent manner (the influence of cell thickness and internal complexity was noted).

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Section: Introductionmentioning

confidence: 99%

Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy

et al. 2020

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“…For medical applications, it is important to quantify and minimize this dose, because it can be harmful to the patient's health. In comparison to medical scans, micro-CT scans have a much higher resolution, hence a smaller voxel size, which typically gives rise to a higher dose in the object under investigation [1,2]. For a given material, the absorbed dose scales roughly linearly with the number of interactions per unit of volume, hence for a fixed signal to noise ratio the dose will scale as (voxel volume) −1 .…”

Section: Introductionmentioning

confidence: 99%

Fast method for the estimation of the absorbed dose in X-ray microtomography

Muynck

Bonte

Dhaene

et al. 2019

Nuclear Instruments and Methods in Physics Research Section B:

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Micro-CT imaging is an increasingly popular tool in the internal investigation of objects and materials. However, as an X-ray based technique, a potentially harmful radiation dose is deposited in the sample during the measurement. In (non small-animal) micro-CT imaging one is dealing with a strong variation in measurement systems and settings, resulting in many different acquisition circumstances and the absence of standard imaging protocols. Therefore, the deposited dose is rarely studied for micro-CT applications. This research aimed at developing a fast simulation technique to predict the dose associated with micro-CT scanning. Its performance is compared with that of two different Monte Carlo simulation tools and with a straight forward approach to estimate an upper limit for the dose. The fast simulation method, obtaining a dose estimation based on the energy absorption coefficient, is much faster than the Monte Carlo simulations, and the results are accurate within 30%. This enables us to predict the dose for a known sample and a known scanner setup, without complex Monte Carlo simulations and will allow researchers to avoid radiation damage or unwanted radiation induced effects, an increasingly important concern in 3D and 4D micro-CT scanning.

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X-ray Microscopy

Jacobsen

2019

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Future challenges for x-ray microscopy

Abstract: Fabrication of hard x-ray zone plates with high aspect ratio using metal-assisted chemical etching

Cited by 18 publications

References 67 publications

Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy

Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy

Fast method for the estimation of the absorbed dose in X-ray microtomography

X-ray Microscopy

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