2016
DOI: 10.1016/j.bpj.2016.01.037
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Future Perspective of Single-Molecule FRET Biosensors and Intravital FRET Microscopy

Abstract: Fö rster (or fluorescence) resonance energy transfer (FRET) is a nonradiative energy transfer process between two fluorophores located in close proximity to each other. To date, a variety of biosensors based on the principle of FRET have been developed to monitor the activity of kinases, proteases, GTPases or lipid concentration in living cells. In addition, generation of biosensors that can monitor physical stresses such as mechanical power, heat, or electric/magnetic fields is also expected based on recent d… Show more

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Cited by 37 publications
(28 citation statements)
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“…To image intracellular protein/protein interactions and protein activity, numerous molecular biosensors were developed based on the principle of FRET. In FRET, energy transfer occurs between two fluorescent proteins from a donor to an acceptor, and its efficiency is dependent on their local proximity, disappearing at distances greater than 10 nm . Most commonly, FRET is detected by collecting emission signals of donor and acceptor proteins, following donor excitation.…”
Section: Cell Fate Mapping Imaging Molecular Activities and Cell‐cementioning
confidence: 99%
See 1 more Smart Citation
“…To image intracellular protein/protein interactions and protein activity, numerous molecular biosensors were developed based on the principle of FRET. In FRET, energy transfer occurs between two fluorescent proteins from a donor to an acceptor, and its efficiency is dependent on their local proximity, disappearing at distances greater than 10 nm . Most commonly, FRET is detected by collecting emission signals of donor and acceptor proteins, following donor excitation.…”
Section: Cell Fate Mapping Imaging Molecular Activities and Cell‐cementioning
confidence: 99%
“…In FRET, energy transfer occurs between two fluorescent proteins from a donor to an acceptor, and its efficiency is dependent on their local proximity, disappearing at distances greater than 10 nm. 72 Previously, the elevated activity of the small G-protein Rac1 was shown to facilitate the hyperproliferation of cells at the base of intestinal crypts, leading to the initiation of colorectal cancer. 74,75 To test this hypothesis in vivo, a Rac-FRET mouse exhibiting pancreatic ductal adenocarcinoma was developed.…”
Section: Imaging Molecular Activities Of Cancer Cellsmentioning
confidence: 99%
“…During FRET, a donor fluorophore is excited and transfers energy to an acceptor fluorophore through a nonradiative process. 75 The energy transfer efficiency is directly related to the intervening distance between the donor and acceptor dyes. Thus, FRET is often referred to as a molecular ruler as it can provide molecular-based distance information between two reporter dyes.…”
Section: −74mentioning
confidence: 99%
“…The use of optical biosensor mice therefore obviates the need to ‘load’ tissues with a fluorescent dye and provides a phenotypically normal mouse in which artery Ca 2+ signaling (or other) and artery diameter can be readily measured. There are advantages also of biosensor mice, since the problems of transient expression in cultured cells (Hirata and Kiyokawa, 2016), noted for FRET studies, under control of strong (e.g CMV) promoters is avoided; expression in biosensor mice can be stable and non-perturbing.…”
Section: In Vivo Imaging Studiesmentioning
confidence: 99%