Future scope of plant-derived bioactive compounds in the management of methicillin-resistant Staphylococcus aureus: In vitro antimicrobial and antivirulence prospects to combat MRSA

Abdel-Raheem, Sherief M.; El-Hamid, Marwa I. Abd; Ibrahim, Doaa; El-Malt, Rania M S; El‐Ghareeb, Waleed Rizk; Ismail, Hesham; Al-Sultan, Saad I.; Meligy, Ahmed; ELTarabili, Reham

doi:10.1016/j.micpath.2023.106301

Cited by 11 publications

(9 citation statements)

References 65 publications

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“…Additionally, the excessive utilization of antimicrobials has serious negative effects including the development of antimicrobial residues in rabbit meat, disturbing the gut microflora and reducing the host defense system ( 16 ). As a result, it is critical to find novel alternative antibiotics such as phytochemicals to control bacterial infections brought on by these MDR strains ( 17 , 18 ).…”

Section: Introductionmentioning

confidence: 99%

Future impact of thymoquinone-loaded nanoemulsion in rabbits: prospects for enhancing growth, immunity, antioxidant potential and resistance against Pasteurella multocida

Abd El-Hamid,

El-Azzouny,

El-Malt

et al. 2024

Front. Vet. Sci.

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Phytochemical nanoemulsions, such as thymoquinone nanoemulsions (TQN), are regarded as innovative alternatives to antimicrobials that significantly improve the performance, digestion, antioxidant potential and immunity of rabbits. Thus, the potential effects of TQN on growth, digestibility, antioxidant potential, immunity and resistance against Pasteurella multocida (P. multocida) in rabbits were assessed. Herein, 240 rabbits were offered either a basal diet or diets fortified with three TQN-graded concentrations. At 60 days of age, rabbits were challenged with multidrug-resistant (MDR) virulent P. multocida strain. Our outcomes described that dietary inclusion of TQN, especially at higher concentrations, significantly enhanced the growth performance of rabbits, which was supported by increasing the levels of jejunal lipase, amylase and trypsin enzymes. Of note, the levels of muscle and jejunal antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and total antioxidant capacity (T-AOC)], serum immunological markers (IgG, IgG, IgM and total Igs) and blood phagocytic percentage were significantly provoked after TQN fortification; meanwhile, the levels of muscle and jejunal MDA, serum biochemical parameters (total cholesterol, TG and LDL), abdominal fat percentage, breast and thigh cholesterol were significantly decreased following TQN supplementations. Our findings showed that TQN protected rabbits against P. multocida experimental challenge as evidenced by reducing P. multocida counts in rabbits’ lungs, downregulating the transcription levels of P. multocida virulence-related genes (ptfA, toxA and nanB) at 48 and 96 h post-infection and ameliorating the expression levels of cytokines-related genes (IL-1β, IL-10, IL-8, IL-6, DEFB1, TNF-α, TLR-4 and TLR-2) at 96 h post-infection. Our findings suggest the utilization of TQN in rabbits’ diets due to their stimulating effects on digestibility as well as their growth-promoting, anti-inflammatory, antioxidant, antibacterial, anti-virulence and immunostimulant properties, which enhance the rabbits’ P. multocida resistance.

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Section: Introductionmentioning

confidence: 99%

Future impact of thymoquinone-loaded nanoemulsion in rabbits: prospects for enhancing growth, immunity, antioxidant potential and resistance against Pasteurella multocida

Abd El-Hamid,

El-Azzouny,

El-Malt

et al. 2024

Front. Vet. Sci.

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“…Natural antimicrobial compounds from EOs could interfere with membrane integrity in bacterial pathogens [ 27 ]. Recent findings have showed the potent in vitro antimicrobial and anti-virulent capacities of trans-cinnamaldehyde, thymol, and carvacrol against these challenging pathogens [ 14 ]. These compounds reduced the growth of multi-virulent and MDR-MRSA isolates and the transcriptional activity of specific virulence genes.…”

Section: Discussionmentioning

confidence: 99%

“…Significantly, sub-inhibitory concentrations (SICs) of trans-cinnamaldehyde, thymol, and carvacrol have been found to induce substantial downregulation of genes such as sea , eta , tst , icaA , and cna in evaluated isolates. Given these insights, the study advocates for the consideration of phytogenic compounds, including trans-cinnamaldehyde, thymol, and carvacrol, as potential alternative antimicrobials to address the growing challenge of MRSA infections [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

The mechanistic role of natural antimicrobials in preventing Staphylococcus aureus invasion of MAC-T cells using an in vitro mastitis model

Balta,

McCleery,

David

et al. 2024

Ir Vet J

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Background Starting primarily as an inflammation of the mammary gland, mastitis is frequently driven by infectious agents such as Staphylococcus aureus. Mastitis has a large economic impact globally, which includes diagnostic, treatment, and the production costs not to mention the potential milk contamination with antimicrobial residues. Currently, mastitis prevention and cure depends on intramammary infusion of antimicrobials, yet, their overuse risks engendering resistant pathogens, posing further threats to livestock. Methods In our study we aimed to investigate, in vitro, using bovine mammary epithelial cells (MAC-T), the efficacy of the AuraShield an antimicrobial mixture (As) in preventing S. aureus attachment, internalisation, and inflammation. The antimicrobial mixture (As) included: 5% maltodextrin, 1% sodium chloride, 42% citric acid, 18% sodium citrate, 10% silica, 12% malic acid, 9% citrus extract and 3% olive extract (w/w). Results and discussion Herein we show that As can significantly reduce both adherence and invasion of MAC-T cells by S. aureus, with no impact on cell viability at all concentrations tested (0.1, 0.2, 0.5, 1%) compared with untreated controls. The anti-apoptotic effect of As was achieved by significantly reducing cellular caspase 1, 3 and 8 activities in the infected MAC-T cells. All As concentrations were proven to be subinhibitory, suggesting that Ac can reduce S. aureus virulence without bacterial killing and that the effect could be dual including a host modulation effect. In this context, we show that As can reduce the expression of S. aureus clumping factor (ClfB) and block its interaction with the host Annexin A2 (AnxA2), resulting in decreased bacterial adherence in infection of MAC-T cells. Moreover, the ability of As to block AnxA2 had a significant decreasing effect on the levels of pro inflammatory cytokine released upon S. aureus interaction with MAC-T cells. Conclusion The results presented in this study indicate that mixtures of natural antimicrobials could potentially be considered an efficient alternative to antibiotics in treating S. aureus induced mastitis.

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resistant S. aureus (MRSA), has been considered a key contributing factor in numerous illnesses worldwide including toxic shock syndrome, septicemia, pneumonia, food-poisoning outbreaks, (Lin and Peterson, 2014;Islam et al, 2019), in addition to subclinical and clinical mastitis in dairy animals (Abdel-Raheem et al, 2023).Recently, the incidence of multidrug-resistant (MDR) bacteria has dramatically increased as a result of antimicrobials abuse, which hindered the healing process (Ammar et al, 2021a, b). The widespread dissemination of S. aureus is owing to its prospective virulence factors as well as its extraordinary capacity to withstand most antimicrobials released in recent years with the development of MDR strains (Ammar et al, 2016).

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mentioning

confidence: 99%

“…resistant S. aureus (MRSA), has been considered a key contributing factor in numerous illnesses worldwide including toxic shock syndrome, septicemia, pneumonia, food-poisoning outbreaks, (Lin and Peterson, 2014;Islam et al, 2019), in addition to subclinical and clinical mastitis in dairy animals (Abdel-Raheem et al, 2023).…”

mentioning

confidence: 99%

Antimicrobial Resistance and Virulence Genes` Profiles of Staphylococcus aureus in Meat and Meat Products

Al-Harthi,

Eldrehmy,

Eladl

et al. 2024

AAVS

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resistant S. aureus (MRSA), has been considered a key contributing factor in numerous illnesses worldwide including toxic shock syndrome, septicemia, pneumonia, food-poisoning outbreaks, (Lin and Peterson, 2014;Islam et al., 2019), in addition to subclinical and clinical mastitis in dairy animals (Abdel-Raheem et al., 2023).Recently, the incidence of multidrug-resistant (MDR) bacteria has dramatically increased as a result of antimicrobials abuse, which hindered the healing process (Ammar et al., 2021a, b). The widespread dissemination of S. aureus is owing to its prospective virulence factors as well as its extraordinary capacity to withstand most antimicrobials released in recent years with the development of MDR strains (Ammar et al., 2016). MRSA has evolved into an aggressive endemic bacterium with adverse effects on public

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Future scope of plant-derived bioactive compounds in the management of methicillin-resistant Staphylococcus aureus: In vitro antimicrobial and antivirulence prospects to combat MRSA

Cited by 11 publications

References 65 publications

Future impact of thymoquinone-loaded nanoemulsion in rabbits: prospects for enhancing growth, immunity, antioxidant potential and resistance against Pasteurella multocida

Future impact of thymoquinone-loaded nanoemulsion in rabbits: prospects for enhancing growth, immunity, antioxidant potential and resistance against Pasteurella multocida

The mechanistic role of natural antimicrobials in preventing Staphylococcus aureus invasion of MAC-T cells using an in vitro mastitis model

Antimicrobial Resistance and Virulence Genes` Profiles of Staphylococcus aureus in Meat and Meat Products

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