Fuzzy clustering demonstrates that codon 72 SNP rs1042522 of TP53 gene associated with HNSCC but not with prognoses

Abstract

It is estimated that 7.6 million people will die as a consequence of head and neck squamous cell carcinoma (HNSCC). Genetic predisposition has emerged as an important risk factor in the development and prognosis of HNSCC. Considering this, the aim of the current study is to assess whether codon 72 SNP of the TP53 gene (rs1042522) is associated with an increased odds ratio of developing HNSCC or with a worse prognosis in patients with HNSCC. Analysis of the rs1042522 in HNSCC patients and in control individuals. Differences between the case and control groups were determined using chi-squared tests. Multivariate analysis was performed to evaluate the odds ratio of HNSCC. Fussy C Means Clustering was to cluster HNSCC patients for survival analyses. Time of survival was calculated using the Kaplan-Meier estimator and comparing this to the log rank test. Statistical significance was set at p < 0.05. A total of 71.4 % of the Arg/Arg genotype were from HNSCC patients, while only 28.6 % of Arg/Arg genotype were found in the control group. Logistic regression demonstrated that the Arg/Arg genotype, smoking, and alcohol consumption increase the odds ratio of HNSCC. No association between TP53 codon 72 polymorphism and P53 expression. No association between rs1042522 and survival or prognoses was observed. This study identified that individuals carrying the arginine allele at rs1042522 have an increased odds ratio of HNSCC. However, no association between codon 72 SNP of the TP53 gene and HNSCC prognosis or P53 expression was observed.

HPV, p16 and p53 As Indicators of Response to Survival in Larynx Squamous Cell Carcinoma

“…Neither P16 nor p53 expression was an independent risk factor affecting the prognosis of laryngeal cancer. This is consistent with previous studies [20,21].…”

Frequencies of TP53 Germline Variations and Identification of Two Novel 3’UTR Variants in a Series of Head and Neck Cancer Cases

“…These comparisons indicate that Arg substitution at codon 72 may be a risk factor for HNC in South-Asian populations. However, this observation is in contrast to the previous studies, which imply that Pro72 in p53 may be associated with increased risk to cancers (Cao et al, 2020, Lotfi Garavand et al, 2020, Pinheiro et al, 2015, Cortezzi et al, 2004. This contradiction may be partially explained by other genetic and/or environmental factors, which have not been considered in the present discovery study.…”

A novel human in vitro papillomavirus type 16 positive tonsil cancer cell line with high sensitivity to radiation and cisplatin

“…Interestingly, this arginine form of the gene product p53 has been shown to be more susceptible to HPV16 E6 mediated degradation than the proline form [35]. In addition, the arginine form of p53 was found to be associated with HNSCC, but not with worse prognosis of the disease [36]. Furthermore, Habbous et al reported in a meta-analysis that the arginine variant of p53 was associated with progression of squamous intraepithelial lesions to cervical cancer only in the presence of HPV positivity [37].…”