Fuzzy Liquid Analysis by an Array of Nonspecifically Interacting Reagents: The Taste of Fluorescence

Hänninen, Pekka; Siivonen, Joonas; Väisänen, Pave; Tiittanen, Satu; Lehmusto, Mirva; Teimonen, Timo; Sandell, Mari; Knaapila, Antti; Mundill, Paul; Härmä, Harri

doi:10.1021/ja401726d

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…This is in line with our study on 20 red wines reported elsewhere indicating that under selected assay conditions and modulators, wines can be identified and grouped according to their origin (Hänninen et al 2013). Therefore, the liquid fingerprint technology can be considered as a potential method for wine identification.…”

Section: Wine Identificationsupporting

confidence: 91%

Novel non‐specific liquid fingerprint technology for wine analysis: a feasibility study

Siivonen¹,

Väisänen²,

Tiittanen³

et al. 2014

Australian Journal of Grape and Wine Research

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Background and Aims: A novel, rapid and simple liquid fingerprinting technology is described and demonstrated for wine identification and for quality control. Method and Results:The wine sample, selected chemical modulators on the surfaces of an array, and a long lifetime luminescent europium label interact non-specifically providing a unique luminescence fingerprint that is highly wine specific. The technique was applied to 15 red wines of different vintages from four European vineyards. The fingerprint data, in addition to identification and after data processing, show a significant correlation with the results from existing Fourier transform infrared spectroscopic and spectrophotometric methods of wine analysis. Conclusions: Identification of individual wines through specific luminescent fingerprints provides a simple and efficient tool to combat wine adulteration and fraud. The same principles combined with proper data processing can enable the monitoring of other parameters such as wine aging. Significance of the Study: This study demonstrates a fast, affordable and rapid test platform for red wine analysis.

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Section: Wine Identificationsupporting

confidence: 91%

Novel non‐specific liquid fingerprint technology for wine analysis: a feasibility study

Siivonen¹,

Väisänen²,

Tiittanen³

et al. 2014

Australian Journal of Grape and Wine Research

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“…Cancer was classified from control samples after 15 min of incubation by measuring the sensor with TRF. Detection was based on modulation in the chemical environment, which affects the capabilities of the label to emit TRF [24]. Additionally, we measured absorbance from the chemical sensor, with a more significant correlation to cancer already after 5 min of incubation.…”

Section: Discussionmentioning

confidence: 99%

Phage Biosensor for the Classification of Metastatic Urological Cancers from Urine

Juusti,

Rannikko,

Laurila

et al. 2024

Life

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Most of the annual 10 million cancer-related deaths are caused by metastatic disease. Survival rates for cancer are strongly dependent on the type of cancer and its stage at detection. Early detection remains a challenge due to the lack of reliable biomarkers and cost-efficient screening methods. Phage biosensors can offer a solution for early detection using non-invasive liquid biopsies. Here, we report the first results of the bifunctional phage biosensor to detect metastatic urological cancers from urine. A dye-sensitized phage library was used to select metastasis-related phage binders. After selection rounds, the most promising phage candidate was used to classify metastatic cancer from controls. Additionally, we applied one chemical sensor (phenoxazine non-fluorescent dye) to classify cancer from urine. A statistical significance (p = 0.0002) was observed between metastatic and non-metastatic cancer, with sensitivity of 70% and specificity of 79%. Furthermore, the chemical sensor demonstrated significance in detecting cancer (p < 0.0001) with a sensitivity of 71% and a specificity of 75%. Our data suggest a new promising field for urine biomarker research, and further evaluation with prospectively collected samples is ongoing. In conclusion, we report, for the first time, the potential of a chemical- and phage-based biosensor method to detect metastatic cancer using urine.

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“…The TRL measurement is very sensitive, mostly due to the environmentally sensitive lanthanide label, which intermediates even small changes in the microenvironment to the measured signal [ 24 ] and eliminates the background luminescence [ 25 ]. This enables reliable and indirect estimation of the GFP analyte concentration in the reaction.…”

Section: Discussionmentioning

confidence: 99%

Biophysical Properties of Bifunctional Phage-Biosensor

Juusti

Kulpakko²,

Cudjoe

et al. 2023

Viruses

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Biosensor research is a swiftly growing field for developing rapid and precise analytical devices for biomedical, pharmaceutical, and industrial use and beyond. Herein, we propose a phage-based biosensor method to develop a sensitive and specific system for biomedical detection. Our method is based on in vitro selected phages and their interaction with the targeted analytes as well as on optical properties that change according to the concentration of the model analyte. The green fluorescent protein (GFP) was chosen as our model analyte as it has its own well-known optical properties. Brilliant green was used as a reporter component for the sensor. Its presence enables a color intensity (absorbance) change when the analyte is present in the solution. Furthermore, the reporter dye functioned as a quencher for an additional lanthanide label in our assay. It mediated the specific phage-derived interference in the signal measured with the time-resolved luminescence. Most importantly, our results confirmed that the presented bifunctional phage with its liquid crystal properties enabled the measurement of GFP in a concentration-dependent, quantitative manner with a limit of detection of 0.24 µg/mL. In the future, our novel method to develop phage-based biosensors may provide highly sensitive and specific biosensors for biomedical or otherwise-relevant targets.

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Fuzzy Liquid Analysis by an Array of Nonspecifically Interacting Reagents: The Taste of Fluorescence

Cited by 6 publications

References 8 publications

Novel non‐specific liquid fingerprint technology for wine analysis: a feasibility study

Novel non‐specific liquid fingerprint technology for wine analysis: a feasibility study

Phage Biosensor for the Classification of Metastatic Urological Cancers from Urine

Biophysical Properties of Bifunctional Phage-Biosensor

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