Broiler house systems are operated for the primary purpose of providing the appropriate conditions suitable to have a significant efficiency of animal production. The major environmental conditions in the poultry building are controlling the hygro-thermal parameters (temperature and relative humidity) and contaminant gases (NH 3 , CO 2). In this paper, a poultry house prototype is monitored and controlled using the Supervisory Control and Data Acquisition (SCADA) tool like LabVIEW. A full prototype is designed and an efficient hybrid control strategy is implemented to control in real-time the poultry house climate. In the suggested approach, a Multi-Input Multi-Output (MIMO) fuzzy logic controller (FLC) is combined with a proportional, integral, derivative (PID) controller tuned by fuzzy rules. The proposed method, fuzzy logic, and On/Off controllers were tested by experimental measures and studies in a prototype model over 30 days during hot climates. The comparison results showed that the root mean square error of temperature and relative humidity response with the MFLPID controller (0.8 C, 1.34%) were lower than that of FLC (1.16 C, 1.86%) and On/Off controller (2.09 C, 3.08%). The mean value of CO 2 concentration with MFLPID (2461 ppm) was lower than that of FLC (3294 ppm) and On/Off controller (3624 ppm). However, the mean value of NH 3 concentration was limited in small value (<5 ppm) for all controllers. The performance of the daily weight gained by the chickens for the MFLPID system was found to be 97%, which is higher than that of FLC (88%) and On/Off (80%). The energy consumption of the actuators can be saved at 43% and 14% with MFLPID compared to the On/Off and fuzzy controllers. These results indicate that the proposed control strategy is more efficient in the application of the poultry farming sector.