FXYD11 mediated modulation of Na+/K+-ATPase activity in gills of the brackish medaka (Oryzias dancena) when transferred to hypoosmotic or hyperosmotic environments

Chang, Chia-Hao; Yang, Wen Kai; Lin, Chia Hao; Kang, Can; Tang, Cheng; Lee, Tsung‐Han

doi:10.1016/j.cbpa.2016.01.013

Cited by 19 publications

(18 citation statements)

References 25 publications

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“…The increase likely occurred as a compensatory response to increase ATPase activity, but the compensatory response cannot offset the toxic effect of DCAcAm on ATPase activity. This observation was also in line with earlier studies, they reported that fish tried to upregulate Na + /K + ‐ATP activity through multiple pathways of compensatory response.…”

Section: Discussionsupporting

confidence: 92%

Chronic exposure to dichloroacetamide induces biochemical and histopathological changes in the gills of zebrafish

Sun

Zhang

Ndayambaje

et al. 2019

Environmental Toxicology

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To evaluate the impact of DCAcAm on zebrafish gill, we measure the responses of antioxidant enzyme (superoxide dismutase, SOD), lipid peroxidation (malondialdehyde, MDA), ATPase (Na+/K+‐ATPase and Ca2+/Mg2+‐ATP) and histopathological changes of gill in adult zebrafish, after exposed to different concentrations of DCAcAm (0, 1, 10, 100, and 1000 μg L−1) for 30 days. Results indicated that DCAcAm first increased and then decreased SOD activity, and DCAcAm also lowered the activities of Na+/K+‐ATPase and Ca2+/Mg2+‐ATPase. These results indicated that high affinity of DCAcAm probably be a main factor, which can damage the structures of enzymes, thereby inhibiting the SOD and ATPase activities. Besides, histopathological investigation results also manifested that chronic exposure to DCAcAm can damage the gill tissues, disrupting the normal function of gills. We conclude that chronic exposure to DCAcAm was harmful to organisms, not only influence gill function, but also further cause damage on the gill tissues.

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Section: Discussionsupporting

confidence: 92%

Chronic exposure to dichloroacetamide induces biochemical and histopathological changes in the gills of zebrafish

Sun

Zhang

Ndayambaje

et al. 2019

Environmental Toxicology

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“…Compared to the mammalian FXYD proteins, the roles of most fish FXYD proteins are unknown to date. In teleosts, salinity-dependent expression has only been investigated in depth for FXYD9, FXYD11, and FXYD12, and their potential roles in osmoregulatory organs have been illustrated (Wang et al, 2008 ; Tipsmark et al, 2010 , 2011 ; Tang et al, 2012 ; Yang et al, 2013 , 2016b ; Hu et al, 2014 ; Chang et al, 2016b ). Hence, the present study investigated the expression and function of TnFXYD8 in pufferfish kidneys to illustrate the mechanisms underlying the regulation of NKA activity in euryhaline teleosts.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, FXYD12 was also found to be expressed with the NKA α-subunit in the renal tubules of two medaka species (Yang et al, 2016b ). Furthermore, salinity-dependent interactions were found for branchial FXYD11 and renal FXYD12 with the NKA α-subunit in the brackish medaka (Chang et al, 2016b ; Yang et al, 2016b ). These results suggested that teleostean FXYD proteins might be able to modulate NKA expression/activity via their interaction, as observed in mammals (Garty and Karlish, 2006 ; Geering, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

“…In fish, FXYD10 was found in shark only and its role in inhibition of NKA activity has been demonstrated (Mahmmoud et al, 2000 , 2003 ). Positive correlations (mRNA and/or protein levels) between FXYD proteins and NKA have been commonly found, thus inferring that these FXYD proteins may induce NKA activity (Tipsmark, 2008 ; Saito et al, 2010 ; Tipsmark et al, 2010 , 2011 ; Tang et al, 2012 ; Yang et al, 2013 , 2016b ; Chang et al, 2016b ). For the functional assay, Saito et al ( 2010 ) first analyzed FXYD11-knockdown in zebrafish and found an increased number of NKA-IR cells to compensate for the functional impairment of NKA.…”

Section: Discussionmentioning

confidence: 99%

“…The intensity of the immunoreactive band of the internal control in the immunoblot was set to one and used as a standard for normalizing relative intensities of other bands in the immunoblots. Relative abundance of target proteins was calculated using the following formula (TnFXYD8 n /actin n )/(TnFXYD8 internal control /actin internal control ) (Chang et al, 2016a , b ).…”

Section: Methodsmentioning

confidence: 99%

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FXYD8, a Novel Regulator of Renal Na+/K+-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis

et al. 2017

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FXYD proteins are important regulators of Na+/K+-ATPase (NKA) activity in mammals. As an inhabitant of estuaries, the pufferfish (Tetraodon nigroviridis) responds to ambient salinity changes with efficient osmoregulation, including alterations in branchial, and renal NKA activities. Previous studies on teleostean FXYDs have mainly focused on the expression and potential functions of FXYD proteins in gills. The goal of the present study was to elucidate the potential role of FXYD8, a member of the fish FXYD protein family, in the modulation of NKA activity in the kidneys of this euryhaline pufferfish by using molecular, biochemical, and physiological approaches. The results demonstrate that T. nigroviridis FXYD8 (TnFXYD8) interacts with NKA in renal tubules. Meanwhile, the protein expression of renal TnFXYD8 was found to be significantly upregulated in hyperosmotic seawater-acclimated pufferfish. Moreover, overexpression of TnFXYD8 in Xenopus oocytes decreased NKA activity. Our results suggest the FXYD8 is able to modulate NKA activity through inhibitory effects upon salinity challenge. The present study further extends our understanding of the functions of FXYD proteins, the regulators of NKA, in vertebrates.

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Na+/K+-ATPase response to salinity change and its correlation with FXYD11 expression in Anguilla marmorata

Liang

Zhang

et al. 2017

J Comp Physiol B

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The Na/K-ATPase (NKA) is a primary electrogenic protein that promotes ion transport in teleosts. FXYD11 is a putative regulatory subunit of the NKA pump. The regulation of Na /K -ATPase and FXYD11 is of critical importance for osmotic homeostasis. To investigate the changes of the two genes under different salinity environments, we first identified NKA (AmNKAα1) and FXYD11 (AmFXYD11) in Anguilla marmorata, and then evaluated the mRNA levels of NKA and FXYD11 as well as the activity of NKA in the gill and kidney at different timepoints (0, 1, 3, 6, 12, 24, 48, 72, 96, and 360 h) under three salinity conditions-0‰ (fresh water: FW), 10‰ (brackish water: BW), and 25‰ (seawater: SW). In the gill, the mRNA levels of AmNKAα1 and AmFXYD11 and the enzyme activity of AmNKAα1 were higher in BW and SW than in FW; the protein abundance was positively correlated with the specific activity of NKA in BW/SW. However, in the kidney, the mRNA level of AmNKAα1 in the BW group was higher than that in the FW group. In addition, AmFXYD mRNA levels in both BW and SW groups were significantly lower than that in the FW control group. These results suggested that AmFXYD11 was tissue specific in response to different salinity environment. Our results clearly demonstrated the important roles of AmNKAα1 and AmFXYD11 in osmotic homeostasis of juvenile A. marmorata under saline environment.

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FXYD11 mediated modulation of Na+/K+-ATPase activity in gills of the brackish medaka (Oryzias dancena) when transferred to hypoosmotic or hyperosmotic environments

Cited by 19 publications

References 25 publications

Chronic exposure to dichloroacetamide induces biochemical and histopathological changes in the gills of zebrafish

Chronic exposure to dichloroacetamide induces biochemical and histopathological changes in the gills of zebrafish

FXYD8, a Novel Regulator of Renal Na+/K+-ATPase in the Euryhaline Teleost, Tetraodon nigroviridis

Na+/K+-ATPase response to salinity change and its correlation with FXYD11 expression in Anguilla marmorata

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