Fӧrster resonance energy transfer analysis of amyloid state of proteins

Trusova, Valeriya; Tarabara, Uliana; Zhytniakivska, Olga; Vus, Kateryna; Gorbenko, Galyna

doi:10.1016/j.bbadva.2022.100059

Cited by 3 publications

(3 citation statements)

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“…[184] FRET is increasingly being used to determine distances, structures, and dynamics of proteins at the ensemble and the single-molecule level. [50,87,[185][186] Intramolecular FRET usually occurs within inter-dye distances from 30 up to 100 Å that is comparable to the dimensions of most biological macromolecules. [174][175][187][188] Figure 5 shows an example of FRET-based measurements of pH-dependent refolding of the anti-apoptotic protein Bcl-xL.…”

Section: Long-range Förster Resonance Energy Transfer For Monitoring ...mentioning

confidence: 99%

Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein‐Lipid Interactions

Kyrychenko,

Ladokhin

2023

The Chemical Record

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Fluorescence spectroscopy provides numerous methodological tools for structural and functional studies of biological macromolecules and their complexes. All fluorescence‐based approaches require either existence of an intrinsic probe or an introduction of an extrinsic one. Moreover, studies of complex systems often require an additional introduction of a specific quencher molecule acting in combination with a fluorophore to provide structural or thermodynamic information. Here, we review the fundamentals and summarize the latest progress in applications of different classes of fluorescent probes and their specific quenchers, aimed at studies of protein folding and protein‐membrane interactions. Specifically, we discuss various environment‐sensitive dyes, FRET probes, probes for short‐distance measurements, and several probe‐quencher pairs for studies of membrane penetration of proteins and peptides. The goals of this review are: (a) to familiarize the readership with the general concept that complex biological systems often require both a probe and a quencher to decipher mechanistic details of functioning and (b) to provide example of the immediate applications of the described methods.

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Section: Long-range Förster Resonance Energy Transfer For Monitoring ...mentioning

confidence: 99%

Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein‐Lipid Interactions

Kyrychenko,

Ladokhin

2023

The Chemical Record

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“…Included among the authors are recipients of various prestigious international awards, including the Gregorio Weber Award in Biological Fluorescence and the Hans Neurath Award in Protein Science. The submissions cover a wide area of subjects, ranging from regulation of translation [ 3 , 4 ] and gene activation [5] to protein-membrane interactions [ 6 , 7 ] and cellular signaling [ 2 , [8] , [9] , [10] , [11] ]; from biotechnology [ 12 , 13 ]to structure-function studies of proteins [14] , [15] , [16] , nucleic acids [17] carbohydrates [18] and phosphoglycolipids [19] . The contributions to this issue also cover a broad spectrum of experimental and theoretical approaches, including photochemistry and biochemistry of proton transfer reactions [ 20 , 21 ], quantum chemical modeling [17] and theoretical and experimental exploration of complex spectroscopic responses of amyloids [6] .…”

mentioning

confidence: 99%

“…The submissions cover a wide area of subjects, ranging from regulation of translation [ 3 , 4 ] and gene activation [5] to protein-membrane interactions [ 6 , 7 ] and cellular signaling [ 2 , [8] , [9] , [10] , [11] ]; from biotechnology [ 12 , 13 ]to structure-function studies of proteins [14] , [15] , [16] , nucleic acids [17] carbohydrates [18] and phosphoglycolipids [19] . The contributions to this issue also cover a broad spectrum of experimental and theoretical approaches, including photochemistry and biochemistry of proton transfer reactions [ 20 , 21 ], quantum chemical modeling [17] and theoretical and experimental exploration of complex spectroscopic responses of amyloids [6] . The presented works clearly demonstrate high level of technical rigor and scientific excellence of Ukrainian researchers in many areas of basic and translational Biosciences.…”

mentioning

confidence: 99%