g.640T&gt;C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens

Muhsinin, M.; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece

doi:10.20884/1.jap.2017.19.2.597

Cited by 7 publications

(8 citation statements)

References 25 publications

(33 reference statements)

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“…Polymerase chain reaction (PCR) amplified a partial region of the TGF-β2 gene, which showed a molecular weight of 284 bp (Figure 2). The present results agree with other authors (21)(22)(23).…”

Section: Pcr Assaysupporting

confidence: 94%

“…It was reported that chickens had two alleles (T and C) and three genotype variants (TT, CT, and CC) for the TGF-β2 gene (21). Polymorphic Indonesian chickens with two alleles (T and C) and three genotypes (TT, CT, and CC) were seen by (23). At the same time, the T allele had a higher prevalence than the C allele in Indonesian chickens owing to inbreeding and vigorous selection of the TGF-β2 gene.…”

Section: Distribution Of Genotype and Allele Frequencymentioning

confidence: 99%

“…A DNA extraction kit was used to obtain DNA from the blood (Favorgen, Taiwan). The primers were supplied from Alpha DNA Canada, as forward 5'-GCC ATA GGT TCA GTG CAA G-3' and reverse 5'-TGA CAG AAG CTC TCA AGC C-3' (21)(22)(23). The components of PCR reaction were prepared according to the procedure suggested by the manufacturing company (Promega, USA) using 12.5 µL master mix, 1 µL forward primer, 1 µL reverse primer, 3 μL of DNA and 7.5 µL distilled water.…”

Section: Sample Collection and Laboratory Analysismentioning

confidence: 99%

“…The substitution of thymine (T) by cytosine (C) in chickens resulted in a change in the RsaI restriction site sequences, which were changed from GT|AC to GC|AC, preventing the RsaI enzyme from recognizing its restriction site (21)(22)(23).…”

Section: Sequencingmentioning

confidence: 99%

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Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens

2021

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Iraqi native chickens have tasty meat and eggs; however, they are characterized by low production efficiency. In fact, phenotypic traits, such as growth rate, are influenced by genes and environmental factors. During health and disease, a variety of cellular processes such as proliferation, differentiation, motility, adhesion, migration, apoptosis, and immune response regulate the TGF-β genes. The enhancement in body weight can be reached through mass selection, whereas feed conversion is relatively more difficult to improve. This means, selecting for body weight has been submitted as an effective way of indirectly improving feed conversion ratio. However, the present study attempts to identify associations between productive traits and polymorphism of TGF-β2 gene in local chicken. Seventy-five male birds were used in this study. The restriction enzyme RsaI has been used to detect the target region (284 bp) in the TGF-β2 gene. A SNP was identified at the 62 position in the exon 1 region of TGF-β2 by using PCR-RFLP and DNA sequencing technique. The genotypic frequencies were 46.7, 40 and 13.3% for CC and TC and TT genotypes, respectively. While the allele frequency of C and T were 0.67 and 0.33%, respectively. Generally, during the last period of rearing the best significant (p>0.05) improve in the body weight, weight gain and FCR were recorded in the TT genotype of the TGF-β2 gene. In conclusion, a functional sequence in the genome could be attributed to the mutation. Therefore, genotype of the TGF-β2 gene could be exploited to select the best individual as a parent to the next generations for improving of growth rate in local chickens.

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Section: Pcr Assaysupporting

confidence: 94%

Section: Distribution Of Genotype and Allele Frequencymentioning

confidence: 99%

Section: Sample Collection and Laboratory Analysismentioning

confidence: 99%

Section: Sequencingmentioning

confidence: 99%

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Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens

2021

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“…Muscle growth in livestock is influenced by three main hormones namely steroids, transforming growth factor-beta (TGF-β), and myostatin (Dayton & White 2007). TGF-β is a family of growth factor hormones that regulate biological activity in a very broad range such as morphogenesis (Lorda-Diez et al 2010), development (Lu et al 2013), production (Jin et al 2013), reproduction (Gu et al 2017), and disease resistance (Muhsinin et al 2017). A previous study by Li et al (2003) showed that TGF-β3 plays a dominant role in growth, especially body weight and average daily gain (ADG).…”

Section: Introductionmentioning

confidence: 99%

The TGF-β3 Gene Polymorphisms and Carcass Components in Kampung x Meat Type Chicken Cross

Khaerunnisa

Jakaria

Sumantri

2020

JIPTHP

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The TGF-β3 gene is a subtype of TGF-β superfamily that plays a crucial role in skeletal muscle growth. The objectives of this study were to identify the TGF-β3|BslI locus polymorphisms and to evaluate their effects on carcass components in Kampung and broiler chicken cross. A total of 129 chickens used in this study, consisting of: Kampung chickens (44), parent stock broilers Cobb strains (10), F1 Kampung x broilers (35), and F2 Kampung x broilers (40). The PCR-RFLP and direct sequencing techniques were used to obtain mutations in the intron 4 of TGF-β3 gene using the BslI restriction enzyme. The TGF-β3|BslI locus was polymorphic in all populations and three genotypes were found: CC, AA, and CA. No association was found between the three genotypes with the carcass components in F2 Kampung x broiler cross.

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Polymorphic characterisation of gallinacin candidate genes and their molecular associations with growth and immunity traits in chickens

Saleh

Khalil

Iraqi

et al. 2020

British Poultry Science

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1. Four gallinacin (GAL) genes were assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to characterise these genes in Fayoumi (F) and Rhode Island Red (R) breeds and their crosses of Rhode Island Red × Fayoumi (½R½F) and Fayoumi × Rhode Island Red (½F½R). 2. Genes examined were GAL2, GAL3, GAL4 and GAL5. The molecular associations between the SNPs of the gallinacin genes and body weight, caecal bacterial count and the serum antibody titres of IgA, IgG and IgM were determined. In the R breed, the frequency of TC genotype was higher than TT and CC genotypes for the GAL3 gene. The GG genotype frequency was higher than AA and AG genotypes for the GAL4 gene in the other genetic groups, and the CA genotype frequency was higher than CC and AA genotypes in crosses for the GAL5 gene. 3. In all populations, the frequency of the C allele was higher than the T allele for the GAL3 gene, the G allele was higher than the A allele for the GAL4 gene and the C allele was higher than the A allele for the GAL5 gene. The observed heterozygosity in R, ½R½F and ½F½R was 0.476, 0.375 and 0.158 for the GAL3 gene, 0.458, 0.615 and 0.250 for the GAL4 gene and 0.053, 0.792 and 0.739 for the GAL5 gene, while the expected heterozygosities were 0.490, 0.430 and 0.145 for the GAL3 gene, 0.430, 0.348 and 0.219 for the GAL4 gene and 0.229, 0.478 and 0.496 for the GAL5 gene, respectively. 4. On a molecular level, the genotype TT was significantly higher for body weight than TC and CC genotypes in the GAL3 gene. Birds with the GG genotype had a significantly lower Salmonella typhimurium count than birds with AA genotype in the GAL4 gene. Birds with the genotype AA had higher significant body weights than those with CC and CA genotypes in the GAL5 gene. 5. The results indicated that the GAL3, GAL4 and GAL5 genes are potential candidates for selection programmes to improve S. typhimurium resistance and body weight in chickens.

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g.640T>C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens

Cited by 7 publications

References 25 publications

Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens

Association of TGF-β2 Gene Polymorphism with Growth Rate in Local Chickens

The TGF-β3 Gene Polymorphisms and Carcass Components in Kampung x Meat Type Chicken Cross

Polymorphic characterisation of gallinacin candidate genes and their molecular associations with growth and immunity traits in chickens

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