2020
DOI: 10.1002/cbin.11398
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G‐CSF‐dependent neutrophil differentiation requires downregulation of MAPK activities through the Gab2 signaling pathway

Abstract: Granulocyte colony-stimulating factor (G-CSF) stimulation of myeloid cells induced tyrosine-phosphorylation of cellular proteins. One of the tyrosine-phosphorylated proteins was found to be a scaffold protein, Grb2-associated binding protein 2 (Gab2). Another member of Gab family protein, Gab3, was exogenously overexpressed in neutrophil progenitor cells to make the Gab3 protein to compete with the endogenous Gab2 for the G-CSF dependent signaling. In Gab3-overexpressed cells, the level of tyrosine phosphoryla… Show more

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Cited by 2 publications
(13 citation statements)
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“…Mouse myeloid cell line GM-I62-1 is a clonal cell line of GM-I62M described previously [4] and its Gab2 functions were characterized [13]. Mouse recombinant interleukin-3 (IL-3) and granulocyte colonystimulating factor (G-CSF) were used as described [36].…”
Section: Growth Factors and Cell Linementioning
confidence: 99%
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“…Mouse myeloid cell line GM-I62-1 is a clonal cell line of GM-I62M described previously [4] and its Gab2 functions were characterized [13]. Mouse recombinant interleukin-3 (IL-3) and granulocyte colonystimulating factor (G-CSF) were used as described [36].…”
Section: Growth Factors and Cell Linementioning
confidence: 99%
“…Anti-phosphotyrosine antibody (4G10) (05-321) (EMD Millipore, MA), anti-SHP2 antibodies (anti-SH-PTP2 (N-16))(sc-424) (Santa Cruz Biotechnologies, TX), anti-phospho-Src family (Tyr416) antibody (#2101) (Cell Signaling Technology, MA), ant-Lyn (44) antibodies (sc-15) (Santa Cruz Biotechnologies, TX), anti-beta-actin (C4) antibody (sc-47778) (Santa Cruz Biotechnologies, TX), Peroxidase-conjugated rabbit anti-mouse immunoglobulins (P-0260)(DakoCytomation, Denmark), Protein A-Horseradish peroxidase-linked (NA9120) (Amersham GE Healthcare, UK) were purchased, respectively. Anti-Gab2 antiserum was described [13]. Anti-p85 subunit of PI3K antiserum was prepared as described below.…”
Section: Chemicals and Antibodiesmentioning
confidence: 99%
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