G. H. Ritterman

Morgan, J.

doi:10.1111/j.1365-2133.1972.tb00323.x

Cited by 9 publications

(13 citation statements)

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“…However, a threshold effect was observed for HRP when Brownian motion and streaming forces were overcome. Such an effect has also been reported for viruses [39], metallic nanoparticles [40], and protein molecules [23]. In a study on the dielectrophoretic collection of RNA, Giraud et al [41] found the expected square dependence on voltage starting at a threshold voltage.…”

Section: Enzyme Immobilizationmentioning

confidence: 62%

Functionality of dielectrophoretically immobilized enzyme molecules

et al. 2013

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The enzyme horseradish peroxidase has been immobilized on nanoelectrode arrays by alternating current dielectrophoresis (DEP). Preservation of its enzymatic function after field application was demonstrated by oxidizing dihydrorhodamine 123 with hydrogen peroxide as co-oxidant to create its fluorescent form, rhodamine 123 (Rh123). Localization of the fluorescently labeled enzyme and its product was conducted by fluorescence microscopy. Nanoelectrodes were prepared as tungsten pins arranged in square arrays. Experimental parameters for dielectrophoretic immobilization were optimized for even enzyme distribution and for enzymatic efficiency. Enzyme activity was quantified by determination of fluorescence intensities of immobilized enzyme molecules and of Rh123 produced. These results demonstrate that DEP can be applied to immobilize enzyme molecules while retaining their activity and rendering any chemical modifications unnecessary. This introduces a novel way for the preparation of bioactive surfaces for processes such as biosensing.

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Section: Enzyme Immobilizationmentioning

confidence: 62%

Functionality of dielectrophoretically immobilized enzyme molecules

et al. 2013

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“…DEP has been employed for flow manipulation of various types of biological particles such as cancerous cells [4][5][6][7][8][9], DNA [13][14][15]53], protein molecules [15,16], white blood cells [54], sperm cells [55], bacteria [33,56], yeast cells [17,33,[57][58][59], virus [60][61][62][63], microalgae [64,65], etc. A review on DEP manipulation of submicron size biological particles has been given by Malyan and Balachandran [66].…”

Section: Dep Separation Of Biological Particlesmentioning

confidence: 99%

“…This type of high throughput cell separation chip could be useful in biomedical analysis systems. Dielectrophoretic trapping of submicron sized virus particles using sawtooth electrode array is reported by Green et al [61] and Morgan and Green [62]. The polarizability of the ellipsoidal virus (tobacco mosaic virus) was calculated con-sidering it as a homogeneous structure.…”

Section: Dep Separation Of Biological Particlesmentioning

confidence: 99%

Dielectrophoretic separation of micron and submicron particles: A review

Dash

Mohanty

2014

Electrophoresis

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This paper provides an overview on separation of micron and submicron sized biological (cells, yeast, virus, bacteria, etc.) and nonbiological particles (latex, polystyrene, CNTs, metals, etc.) by dielectrophoresis (DEP), which finds wide applications in the field of medical and environmental science. Mathematical models to predict the electric field, flow profile, and concentration profiles of the particles under the influence of DEP force have also been covered in this review. In addition, advancements made primarily in the last decade, in the area of electrode design (shape and arrangement), new materials for electrode (carbon, silicon, polymers), and geometry of the microdevice, for efficient DEP separation of particles have been highlighted.

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“…where dl is a dummy integration variables. Morgan and Green proposed a model to find the dielectrophoretic force acting on a rod shaped particle (e.g., virus) and the expression for calculating the DEP force is given by [51]:…”

Section: Simplified Modelmentioning

confidence: 99%

Dielectrophoretic separation of bioparticles in microdevices: A review

2014

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In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological entities/particles suspended in a buffer medium. Dielectrophoretic forces acting on particles depend on various parameters, for example, charge of the particle, geometry of the device, dielectric constant of the medium and particle, and physiology of the particle. Therefore, to design an effective micro-/nanofluidic separation platform, it is necessary to understand the role of the aforementioned parameters on particle motion. In this paper, we review studies particularly related to dielectrophoretic separation in microfluidic devices. Both experimental and theoretical works by several researchers are highlighted in this article covering AC and DC DEP. In addition, AC/DC DEP, which uses a combination of low frequency AC and DC voltage to manipulate bioparticles, has been discussed briefly. Contactless DEP, a variation of DC DEP in which electrodes do not come in contact with particles, has also been reviewed. Moreover, dielectrophoretic force-based field flow fractionations are featured to demonstrate the bioparticle separation in microfluidic device. In numerical front, a comprehensive review is provided starting from the most simplified effective moment Stokes-drag (EMSD) method to the most advanced interface resolved method. Unlike EMSD method, recently developed advanced numerical methods consider the size and shape of the particle in the electric and flow field calculations, and these methods provide much more accurate results than the EMSD method for microparticles.

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G. H. Ritterman

Cited by 9 publications

References 0 publications

Functionality of dielectrophoretically immobilized enzyme molecules

Functionality of dielectrophoretically immobilized enzyme molecules

Dielectrophoretic separation of micron and submicron particles: A review

Dielectrophoretic separation of bioparticles in microdevices: A review

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