G-protein-coupled receptor controls steroid hormone signaling in cell membrane

Wang, Di

doi:10.1603/ice.2016.107278

Cited by 5 publications

(19 citation statements)

References 18 publications

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“…Previous work indicated that GRK2 interacted with ErGPCR-2 in the cell membrane 8 . To confirm this finding, the co-localization of ErGPCR-2-CFP and GRK2-YFP and Ap-FRET experiments were further performed.…”

Section: Resultsmentioning

confidence: 95%

“…We knocked down ErGPCR-1 that transmits 20E signal 7 and ErGPCR-2 that controls 20E entrance to determine which GPCR participated in 20E-induced GRK2 membrane translocation 8 . ErGPCR-1 knockdown had no effect on the 20E-induced GRK2 membrane localization; however, ErGPCR-2 knockdown repressed the 20E-induced GRK2 membrane localization.…”

Section: Resultsmentioning

confidence: 99%

“…In the current study, we demonstrated that the steroid hormone 20E upregulated GRK2 expression via EcRB1 and USP1, and that the increased levels of GRK2 led to the phosphorylation and internalization of ErGPCR-2 because of 20E induction. The internalization of ErGPCR-2 resulted in the reduction of the steroid hormone signal because ErGPCR-2 is necessary for the entrance of the 20E analog [ 3 H]Pon A into the cells 8 . This hypothesis was supported by the observations that 20E pathway genes were upregulated and precocious pupation was promoted after knockdown of GRK2 .…”

Section: Discussionmentioning

confidence: 99%

“…In Helicoverpa armigera , the ecdysone-responsive GPCR1 (ErGPCR-1) transmits 20E signals in the cell membrane, including rapid cellular calcium increase, protein phosphorylation, and subcellular translocation, which regulate the formation of ecdysone nuclear receptor/ultraspiracle protein (EcR/USP) transcription complex, gene transcription, and metamorphosis 7 . Another GPCR, ErGPCR-2, transmits 20E signals from the cell membrane to the nucleus for gene expression 8 . ErGPCR-2 localizes in the cell membrane and controls the entrance of 20E analog [ 3 H]Pon A into the cells.…”

mentioning

confidence: 99%

“…ErGPCR-2 localizes in the cell membrane and controls the entrance of 20E analog [ 3 H]Pon A into the cells. GRK2 directly phosphorylates ErGPCR-2 and induces the internalization of ErGPCR-2, thereby decreasing the entrance of 20E and terminating the 20E signal 8 . However, the mechanism of GRK2 activation in termination of steroid hormone signals remains unclear.…”

mentioning

confidence: 99%

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G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling

Zhao

Wang

Liu

et al. 2016

Sci Rep

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G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. However, the role and mechanism of GRKs in the desensitization of steroid hormone signaling are unclear. In this study, we propose that GRK2 is phosphorylated by protein kinase C (PKC) in response to induction by the steroid hormone 20-hydroxyecdysone (20E), which determines its translocation to the cell membrane of the lepidopteran Helicoverpa armigera. GRK2 protein expression is increased during the metamorphic stage because of induction by 20E. Knockdown of GRK2 in larvae causes accelerated pupation, an increase in 20E-response gene expression, and advanced apoptosis and metamorphosis. 20E induces translocation of GRK2 from the cytoplasm to the cell membrane via steroid hormone ecdysone-responsive GPCR (ErGPCR-2). GRK2 is phosphorylated by PKC on serine 680 after induction by 20E, which leads to the translocation of GRK2 to the cell membrane. GRK2 interacts with ErGPCR-2. These data indicate that GRK2 terminates the ErGPCR-2 function in 20E signaling in the cell membrane by a negative feedback mechanism.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling

Zhao

Wang

Liu

et al. 2016

Sci Rep

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Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

Doria

Hannapel

Pfenninger

2021

Preprint

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Evolutionary processes and acquired tolerance to toxicants are important factors governing how animals respond to chemical exposure. Evidence for increased tolerance to cadmium (Cd), a widely distributed toxic metal in aquatic environments, in Chironomus riparius is conflicting and still questioned if it happens through phenotypic plasticity or genetic adaptation. The present study considered the relevance of directional environmental changes by increasing contaminant concentration in a multigenerational selection experiment. Evaluation of measurable life-cycle traits, transcriptomic responses and quantitative genetics from an evolve and resequencing (E&R) experiment were integrated to assess the potential of C. riparius to adapt to Cd. Survival tests revealed some adaptation to Cd exposure. Genomic analyses showed a strong, genome-wide selective response in all replicates, emphasizing that even control laboratory conditions continually exert selective pressure. The integration of transcriptomic and genomic data could isolate the genes related to Cd acquired resistance. Those genes could be linked to an efflux of metals. Therefore, it is possible to conclude that C. riparius can endure long-term Cd exposure also through genetic adaptation.

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Steroid hormone 20‐hydroxyecdysone promotes CTL1‐mediated cellular immunity in Helicoverpa armigera

Wang

Liu

et al. 2020

Insect Science

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Mermithid nematodes, such as Ovomermis sinensis, are used as biological control agents against many insect pests, including cotton bollworm (Helicoverpa armigera). However, given the host's robust immune system, the infection rate of O. sinensis is low, thus restricting its widespread use. To understand the host defense mechanisms against mermithid nematodes, we identified and characterized a protein involved in the recognition of O. sinensis, the potential O. sinensis-binding protein C-type lectin 1 (HaCTL1a and/or HaCTL1b), which was eluted from the surface of O. sinensis after incubation with H. armigera plasma. HaCTL1b is homologous to the previously reported HaCTL1a protein. HaCTL1 was predominantly expressed in hemocytes and was induced by the steroid hormone 20-hydroxyecdysone through ecdysone receptor (HaEcR) or ultraspiracle (HaUSP), or both. Binding assays confirmed the interactions of the HaCTL1 proteins with O. sinensis but not with Romanomermis wuchangensis, a parasitic nematode of mosquito. Moreover, the HaCTL1 proteins were secreted into the hemocoel and promoted hemocyte-mediated encapsulation and phagocytosis. A knockdown of HaEcR and/or HaUSP resulted in compromised encapsulation and phagocytosis. Thus, HaCTL1 appears to modulate cellular immunity in the defense against parasitic nematodes, and the 20-hydroxyecdysone-HaEcR-HaUSP complex is involved in regulating the process.

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G-protein-coupled receptor controls steroid hormone signaling in cell membrane

Cited by 5 publications

References 18 publications

G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling

G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling

Detecting Cd adaptation footprint in C. riparius with a multi-genomic approach

Steroid hormone 20‐hydroxyecdysone promotes CTL1‐mediated cellular immunity in Helicoverpa armigera

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