2007
DOI: 10.1016/j.imlet.2007.08.007
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G protein-coupled receptors and the modification of FcɛRI-mediated mast cell activation

Abstract: By releasing multiple pro-inflammatory mediators upon activation, mast cells are critical effector cells in the pathogenesis of allergic inflammation. The traditional viewpoint of antigen-dependent mast cell activation is that of a Th 2 -driven process whereby antigen-specific IgE molecules are produced by B cells followed by binding of the IgE to high affinity IgE receptors (FcεRI) expressed on mast cells. Subsequent antigen-dependent aggregation of the FcεRI initiates an intracellular signalling cascade that… Show more

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Cited by 117 publications
(135 citation statements)
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References 134 publications
(213 reference statements)
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“…Activation of C3a and S1P (sphingosine-1-phosphate) receptors could induce degranulation directly [22][23][24]. However, adenosine and prostaglandin E2 (PGE2) alone could not induce degranulation, but they enhanced antigen-induced mast cell degranulation via the A 3 AR and EP3 prostanoid receptor, respectively [1,5].…”
Section: Discussionmentioning
confidence: 99%
“…Activation of C3a and S1P (sphingosine-1-phosphate) receptors could induce degranulation directly [22][23][24]. However, adenosine and prostaglandin E2 (PGE2) alone could not induce degranulation, but they enhanced antigen-induced mast cell degranulation via the A 3 AR and EP3 prostanoid receptor, respectively [1,5].…”
Section: Discussionmentioning
confidence: 99%
“…For degranulation, cytokine release, and signaling studies, BMMCs were sensitized overnight with anti-mouse monoclonal dinitrophenyl (DNP)-IgE (100 ng/ml) (Sigma) in IL-3-free RPMI medium and then rinsed with HEPES buffer (10 4 •7H 2 O) containing 0.04% bovine serum albumin (BSA) (Sigma). In the case of preincubation with pertussis toxin (PTX) (Sigma), BMMCs were sensitized overnight and then pre-incubated with PTX (1 μg/ml) for 4 hours before antigen (DNP-human serum albumin) (DNP-HSA) or PGE 2 or antigen/PGE 2 was added.…”
Section: Cell Activation Degranulation and Cytokine Productionmentioning
confidence: 99%
“…Synergistic responses mediated by FcεRI and GPCRs have been reported in both rodent and human mast cells [10]. However, as studies in human mast cells may be complicated by the additional inhibitory responses with GPCRs such as adenosine and PGE 2 [10], we elected to utilize mouse BMMCs for our studies.…”
Section: Potentiation Of Fcεri-mediated Degranulation Cytokine Produmentioning
confidence: 99%
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