G-Protein-Gated Potassium Channels Containing Kir3.2 and Kir3.3 Subunits Mediate the Acute Inhibitory Effects of Opioids on Locus Ceruleus Neurons

Torrecilla, Rosa Cañada; Marker, Cheryl L.; Cintora, Stephanie C.; Stoffel, Markus; Williams, John T.; Wickman, Kevin

doi:10.1523/jneurosci.22-11-04328.2002

Cited by 184 publications

(183 citation statements)

References 36 publications

Supporting

Mentioning

173

Contrasting

Unclassified

Order By: Relevance

“…Kir3.3-deficient mice are impaired in their ability to mediate the acute inhibitory effect of opioids on locus coeruleus neurons (26) and exhibit a less severe withdrawal from sedative hypnotics such as pentobarbital and ethanol (27), suggesting a role of Kir3.3 in addiction. NCAM-deficient mice show abnormal responses in fear conditioning and addictive responses (28 -30), and TrkB has been associated with antisocial alcohol and nicotine dependence (31,32) as well as anxiety-related behavior (33).…”

Section: Discussionmentioning

confidence: 99%

Functional Consequences of the Interactions among the Neural Cell Adhesion Molecule NCAM, the Receptor Tyrosine Kinase TrkB, and the Inwardly Rectifying K+ Channel KIR3.3

Kleene

Cassens

Bähring

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Functional Consequences of the Interactions among the Neural Cell Adhesion Molecule NCAM, the Receptor Tyrosine Kinase TrkB, and the Inwardly Rectifying K+ Channel KIR3.3

Kleene

Cassens

Bähring

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…3d) recognized a prominent ~58 kDa band in brain and RPE and a fainter ~58 kDa band in retina, consistent with detection of a Kir3.1 monomer (predicted molecular mass, 57 kDa). The slower migrating band of ~75 kDa in RPE may represent differentially glycosylated version of the core polypeptide (Torrecilla et al, 2002). A goat antiKir3.1 polyclonal antibody (sc-18708; Santa Cruz Biotechnology) labeled a single band <25 kDa in human kidney, brain, RPE, and retina and was judged to be non-specific (not shown).…”

Section: Expression Of Kir Proteins In Native Human Rpe and Neural Rementioning

confidence: 99%

Expression of inwardly rectifying potassium channel subunits in native human retinal pigment epithelium

Yang

Zhang

Hughes

2008

Experimental Eye Research

View full text Add to dashboard Cite

Previously, we demonstrated that the inwardly rectifying K + (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that in addition to Kir7.1, 7 other Kir channel subunits (Kir1.1, Kir2.1, Kir2.2, Kir3.1, Kir3.4, Kir4.2 and Kir6.1) are expressed in the RPE, whereas in neural retina, all 14 of the Kir channel subunits examined are expressed. The identities of RT-PCR products in the RPE were confirmed by DNA sequencing. Real-time RT-PCR analysis showed, however, that transcripts of these channels are significantly less abundant than Kir7.1 in the RPE. Western blot analysis of the Kir channel subunits detected in the RPE by RT-PCR revealed the expression of Kir2.1, Kir3.1, Kir3.4, Kir4.2, Kir6.1, and possibly Kir2.2, but not Kir1.1, in both human RPE and neural retina. Our results indicate that human RPE expresses at least 5 other Kir channel subtypes in addition to Kir7.1, suggesting that multiple members of the Kir channel family may function in this epithelium.

show abstract

“…Horizontal brain slices (200-300 μm) were prepared from 26 mice (11 Dbh −/− mice and 15 Dbh +/− mice) as described previously (Torrecilla et al, 2002). The experimenter was blind to the genotype of the animal until after the experiments were completed.…”

Section: Slice Recordingsmentioning

confidence: 99%

Electrophysiological properties of catecholaminergic neurons in the norepinephrine-deficient mouse

Paladini

Beckstead

2007

Neuroscience

View full text Add to dashboard Cite

To determine how norepinephrine affects the basic physiological properties of catecholaminergic neurons, brain slices containing the Substantia Nigra Pars Compacta and Locus Coeruleus were studied with cell-attached and whole-cell recordings in control and dopamine β-hydroxylase knockout (Dbh −/−) mice that lack norepinephrine. In the cell-attached configuration, the spontaneous firing rate and pattern of Locus Coeruleus neurons recorded from Dbh −/− mice was the same as the firing rate and pattern recorded from heterozygous littermates (Dbh +/−). During whole-cell recordings, synaptic stimulation produced an α-2 receptor-mediated outward current in the Locus Coeruleus of control mice that was absent in Dbh −/− mice. Normal α-2 mediated outward currents were restored in Dbh −/− slices after pre-incubation with norepinephrine. Locus Coeruleus neurons also displayed similar changes in holding current in response to bath application of norepinephrine, UK 14304, and methionine-enkephalin. Dopamine neurons recorded in the Substantia Nigra Pars Compacta similarly showed no differences between slices harvested from Dbh −/− and control mice. These results indicate that endogenous norepinephrine is not necessary for the expression of catecholaminergic neuron firing properties or responses to direct agonists, but is necessary for auto-inhibition mediated by indirect α-2 receptor stimulation. Keywordsdopamine β-hydroxylase knockout; locus coeruleus; substantia nigra pars compacta; cocaine The activity of norepinephrine (NE) neurons in the locus coeruleus (LC) has an important role in selective attention, general arousal, and stress reactions upon challenging environmental situations (Foote et al., 1983;Levine et al., 1990;Berridge and Waterhouse, 2003;Aston-Jones and Cohen, 2005), and NE depletion may underlie some affective disorders and disease states (Ressler and Nemeroff, 1999). In addition, the LC projects directly to, and modulates the activity of, midbrain dopamine (DA) neurons (Swanson and Hartman, 1975;Jones and Moore, 1977;Liprando et al., 2004), which are critical for reward and motor related behaviors (Girault and Greengard, 2004;Montague et al., 2004). In an intact animal, NE neurons recorded in vitro fire spontaneously in a single-spike Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. pattern at a rate of up to 5 spikes per second (Williams et al., 1984). However, it is not known whether NE depletion affects the basic physiological properties of NE or DA neurons. NIH Public AccessDopamine β-hydroxylase knockout (Dbh −/−) mice completely lack NE and have many bra...

show abstract

G-Protein-Gated Potassium Channels Containing Kir3.2 and Kir3.3 Subunits Mediate the Acute Inhibitory Effects of Opioids on Locus Ceruleus Neurons

Cited by 184 publications

References 36 publications

Functional Consequences of the Interactions among the Neural Cell Adhesion Molecule NCAM, the Receptor Tyrosine Kinase TrkB, and the Inwardly Rectifying K+ Channel KIR3.3

Functional Consequences of the Interactions among the Neural Cell Adhesion Molecule NCAM, the Receptor Tyrosine Kinase TrkB, and the Inwardly Rectifying K+ Channel KIR3.3

Expression of inwardly rectifying potassium channel subunits in native human retinal pigment epithelium

Electrophysiological properties of catecholaminergic neurons in the norepinephrine-deficient mouse

Contact Info

Product

Resources

About