G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Xi, Hui; Juhas, Mario; Zhang, Yang

doi:10.1016/j.bios.2020.112494

Cited by 99 publications

(57 citation statements)

References 121 publications

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“…Previous reports have employed a similar strategy to detect both human and plant viruses (Yang et al, 2017;Kim et al, 2018;Wang et al, 2019), and a recent report has suggested the potential use of DNAzyme sensors for SARS-CoV-2 detection (Xi et al, 2020). We here demonstrate the utility of this approach for SARS-CoV-2.…”

Section: Discussionmentioning

confidence: 58%

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

Anantharaj

Das

Sharanabasava

et al. 2020

Front. Mol. Biosci.

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The gold standard for the diagnosis of SARS-CoV-2, the causative agent of COVID-19, is real-time polymerase chain reaction (PCR), which is labor-intensive, expensive, and not widely available in resource-poor settings. Therefore, it is imperative to develop novel, accurate, affordable, and easily accessible assays/sensors to diagnose and isolate COVID-19 cases. To address this unmet need, we utilized the catalytic potential of peroxidase-like DNAzyme and developed a simple visual detection assay for SARS-CoV-2 RNA using a conventional thermal cycler by the PCR-induced generation of DNAzyme sensor. The performance of RT-PCR DNAzyme-based sensor was comparable to that of real-time PCR. The pilot scale validation of RT-PCR DNAzyme-based sensor has shown ~100% sensitivity and specificity in clinical specimens (nasopharyngeal swab, n = 34), with a good correlation (Spearman r = 0.799) with the Ct-value of fluorescence probe-based real-time PCR. These findings clearly indicate the potential of this inexpensive, sensitive, and specific molecular diagnostic test to extend our testing capabilities for the detection of SARS-CoV-2 to curtail COVID-19 transmission.

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Section: Discussionmentioning

confidence: 58%

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

Anantharaj

Das

Sharanabasava

et al. 2020

Front. Mol. Biosci.

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“…. In this way, the RT-LAMP method’s sensitivity for detecting SARS-CoV-2 RNA in clinical samples with CT < 30 was confirmed [ 83 ]. Zhang et al also investigated a colorimetric LAMP biosensor.…”

Section: Biomarkers and Indicatorsmentioning

confidence: 94%

“…Mouse anti-human IgG antibody (MHIgG) and rabbit IgG (RIgG) were used to functionalize LNPs. By dispensing a recombinant nucleocapsid phosphoprotein of SARS-CoV-2 onto a nitrocellulose membrane, the specific IgG of SARS-CoV-2 was captured Anti-SARV-CoV-2 IgG - Reverse transcription loop-mediated isothermal amplification (RT-LAMP) [ 83 ] A two-color RT-LAMP assay protocol was utilized to detect the SARS-CoV-2 N gene in clinical samples. The color of the phenol-red dye was changed within 30 min of the reaction in the presence of positive clinical samples with a CT of less than 30 SARS-CoV-2 RNA (N gene) Cycle threshold (CT) less than 30 Colorimetric Loop-Mediated Isothermal Amplification (LAMP) biosensor [ 84 ] Five LAMP primers sets were designed to detect the SARS-CoV-2 RNA (ORF1a gene and Gene N).…”

Section: Biomarkers and Indicatorsmentioning

confidence: 99%

Nanomaterials to tackle the COVID-19 pandemic

Pishva

Yüce

2021

emergent mater.

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The rapid worldwide spread of the COVID-19 pandemic, caused by the severe acute respiratory SARS-CoV-2, has created an urgent need for its diagnosis and treatment. As a result, many researchers have sought to find the most efficient and appropriate methods to detect and treat the SARS-CoV-2 virus over the past few months. Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing is currently used as one of the most reliable methods to detect the new virus; however, this method is time-consuming, labor-intensive, and requires trained laboratory workers. Moreover, despite its high sensitivity and specificity, false negatives are reported, especially in non-nasopharyngeal swab samples that yield lower viral loads. Therefore, designing and employing faster and more reliable methods seems necessary. In recent years, many attempts have been made to fabricate various nanomaterial-based biosensors to detect viruses and bacteria in clinical samples. The use of nanomaterials plays a significant role in improving the performance of biosensors. Plasmonic biosensors, field-effect transistor (FET)-based biosensors, electrochemical biosensors, and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods are only some of the effective ways to detect viruses. However, to use these biosensors to detect the SARS-CoV-2 virus, modifications must be performed to increase sensitivity and speed of testing due to the rapidly spreading nature of SARS-CoV-2, which requires an early point of care detection and treatment for pandemic control. Several studies have been carried out to show the nanomaterial-based biosensors' performance and success in detecting the novel virus. The limit of detection, accuracy, selectivity, and detection speed are some vital features that should be considered during the design of the SARS-CoV-2 biosensors. This review summarizes various nanomaterials-based sensor platforms to detect the SARS-CoV-2, and their design, advantages, and limitations.

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“…Initially, a combination of computed tomography (CT) scans, next-generation sequencing, Polymerase Chain Reactions (RT-qPCR), ELISA and CRISPR based diagnostic approaches were used to scan and detect SARS-CoV-2 [ 2 , 6 ]. These molecular methods (RT-PCR, CRISPR) are more appropriate than syndromic testing and CT scans for correct identifications of virus [ 7 ]. Though, CT scanners are restricted to bigger hospitals, but cannot differentiate a particular virion from different viruses.…”

Section: Introductionmentioning

confidence: 99%

Detecting and inactivating severe acute respiratory syndrome coronavirus-2 under the auspices of electrochemistry

Ashraf¹,

Aziz²,

Qaisrani³

et al. 2021

Current Research in Chemical Biology

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The recent epidemic of novel coronavirus (nCOVID-19) has turn out to be a huge public health concern owing to its fast transmission. Rapid and cost-effective detection of SARS-CoV-2 is crucial to classify diseased individuals. Serological examination based on antibody chromatography as a substitute to RT-PCR provides inadequate help owing to sophisticated personnel, false-positive results, special equipment and high cost. Biosensing techniques provide sensitive and specific detection, recognition and quantification of pathogens. Herein, after an introduction, we review potential electrochemical (EC) biosensors for COVID-19 diagnosis, emphasizing plasmonic, optical, colorimetric and aptamer-based sensors with a special focus on EC biosensors and point-of-care (POC) diagnostic methods. We have conferred the working principle of these biosensors, EC performance in terms of particular analytical figures of merit and their real-time applications in biological matrices. Lastly, we have described briefly the inactivation of SARS-CoV-2 by EC oxidation. In the end, we have concluded this review by clearing up the strengths and weaknesses of EC sensors and future directions. Advancement in research and technology would be our unsurpassed weapons in the fight against COVID-19 and preventing imminent pandemics.

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G-quadruplex based biosensor: A potential tool for SARS-CoV-2 detection

Cited by 99 publications

References 121 publications

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor

Nanomaterials to tackle the COVID-19 pandemic

Detecting and inactivating severe acute respiratory syndrome coronavirus-2 under the auspices of electrochemistry

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