G1 Dynamics at the Crossroads of Pluripotency and Cancer

Fleifel, Dalia; Cook, Jeanette Gowen

doi:10.3390/cancers15184559

Cited by 5 publications

(3 citation statements)

References 149 publications

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“…4A-C). The MYC Proto-Oncogene, BHLH Transcription Factor (C-MYC) gene regulates the transition of cells from G1 to S phase [11]. Protein p21 can inhibit the activity of cyclinE-Cyclin Dependent Kinase 2 (CDK2), thus causing the cell cycle to stall in the G1 phase [12].…”

Section: Knocking Down C8orf59 Induces Signi Cant Cell Cycle Arrestmentioning

confidence: 99%

C8ORF59 regulates ribosome biogenesis to affect progression in lung adenocarcinoma

Pan,

Liao,

et al. 2024

Preprint

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Elevated ribosome biogenesis was required by tumor growth. In this study, we initially screened a set of key genes related to ribosome biogenesis from the GSEA dataset. Then, we obtained differentially expressed gene sets between cancer tissues and adjacent non-cancerous tissues from the GSE datasets. By intersecting these gene sets, we identified potential genes that may play a significant role in the progression of lung adenocarcinoma. Subsequently, through extensive literature review, we finally identified the gene Chromosome 8 Open Reading Frame 59 (C8ORF59) as an interesting candidate. Our research findings demonstrated that the knockdown of C8ORF59 significantly inhibits the migration, invasion potential, cell growth, and clonogenicity of lung adenocarcinoma cells. Additionally, apoptosis assays revealed a significant increase in apoptosis, including both early and late stages, in lung adenocarcinoma cells upon C8ORF59 knockdown. Cell cycle analysis showed that C8ORF59 knockdown arrests cells predominantly in the G0/G1 phase, indicating inhibited cell proliferation. Moreover, knocking down C8ORF59 significantly inhibits the in vivo growth of lung cancer cells. Mechanistically, downregulation of C8ORF59 significantly decreases the expression of 47S rRNA, a component associated with ribosome assembly, ribosome proteins Fibrillarin (FBL) and Ribosomal Protein L3 (RPL3). Additionally, ribosomal biogenesis targeting drugs CX-5461 and C8ORF59 loss generate synergistic effects on key proteins regulating cell cycle and apoptosis. Knocking down C8ORF59 also substantially enhanced the sensitivity of lung adenocarcinoma cells to the chemotherapeutic drug gemcitabine, suggesting a potential association between C8ORF59 and drug resistance. Collectively, these studies suggest the close involvement of C8ORF59 in the progression of lung adenocarcinoma, providing new insights for its therapeutic intervention.

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Section: Knocking Down C8orf59 Induces Signi Cant Cell Cycle Arrestmentioning

confidence: 99%

C8ORF59 regulates ribosome biogenesis to affect progression in lung adenocarcinoma

Pan,

Liao,

et al. 2024

Preprint

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“…Cyclins can be categorized based on the specific phase of the cell cycle in which they predominantly exert their essential functions. In the context of somatic cell biology, it is crucial to recognize the four distinct phases: G1 (Gap 1), S phase (Synthesis), G2 (Gap 2) phase (collectively known as interphase), and M phase (Mitosis) (Figure 2) [18][19][20].…”

Section: Primary Biological Functions and Regulatory Mechanisms Of Cy...mentioning

confidence: 99%

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Szymański,

Bonowicz,

Antosik

et al. 2024

Cancers

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Endometriosis is a gynecological condition where endometrium-like tissue grows outside the uterus, posing challenges in understanding and treatment. This article delves into the deep cellular and molecular processes underlying endometriosis, with a focus on the crucial roles played by cyclins and cytoskeletal proteins in its pathogenesis, particularly in the context of Epithelial–Mesenchymal Transition (EMT). The investigation begins by examining the activities of cyclins, elucidating their diverse biological roles such as cell cycle control, proliferation, evasion of apoptosis, and angiogenesis among ectopic endometrial cells. A comprehensive analysis of cytoskeletal proteins follows, emphasizing their fundamental biological roles and their specific significance to endometriotic cell features. This review sheds light on the interconnected pathways through which cyclins and cytoskeletal proteins converge, contributing to the genesis and progression of endometriosis. Understanding these molecular complexities not only provides insight into the underlying causes of the disease but also holds promise for the development of specific therapeutic approaches, ushering in a new era in the management of this devastating disorder.

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“…MSCs can self-renew, meaning they can divide and maintain their undifferentiated state over extended periods of time. This self-renewal capacity allows MSCs to replenish the stem cell pool and maintain their stem cell properties [ 24 ]. In addition, MSCs exhibit multilineage differentiation potential, meaning they can give rise to various cell types of the mesenchymal lineage, including osteoblasts (bone cells), chondrocytes (cartilage cells), adipocytes (fat cells), and myocytes (muscle cells).…”

Section: The Self-renewal and Differentiation Capabilities Of Mscs In...mentioning

confidence: 99%

Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery

Matsuzaka,

Yashiro

2024

Pharmaceuticals

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Mesenchymal stem cells (MSCs) have emerged as a promising approach for drug delivery strategies because of their unique properties. These strategies include stem cell membrane-coated nanoparticles, stem cell-derived extracellular vesicles, immunomodulatory effects, stem cell-laden scaffolds, and scaffold-free stem cell sheets. MSCs offer advantages such as low immunogenicity, homing ability, and tumor tropism, making them ideal for targeted drug delivery systems. Stem cell-derived extracellular vesicles have gained attention for their immune properties and tumor-homing abilities, presenting a potential solution for drug delivery challenges. The relationship between MSC-based drug delivery and the self-renewal and differentiation capabilities of MSCs lies in the potential of engineered MSCs to serve as effective carriers for therapeutic agents while maintaining their intrinsic properties. MSCs exhibit potent immunosuppressive functions in MSC-based drug delivery strategies. Stem cell-derived EVs have low immunogenicity and strong therapeutic potential for tissue repair and regeneration. Scaffold-free stem cell sheets represent a cutting-edge approach in regenerative medicine, offering a versatile platform for tissue engineering and regeneration across different medical specialties. MSCs have shown great potential for clinical applications in regenerative medicine because of their ability to differentiate into various cell types, secrete bioactive factors, and modulate immune responses. Researchers are exploring these innovative approaches to enhance drug delivery efficiency and effectiveness in treating various diseases.

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G1 Dynamics at the Crossroads of Pluripotency and Cancer

Cited by 5 publications

References 149 publications

C8ORF59 regulates ribosome biogenesis to affect progression in lung adenocarcinoma

C8ORF59 regulates ribosome biogenesis to affect progression in lung adenocarcinoma

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Current Strategies and Therapeutic Applications of Mesenchymal Stem Cell-Based Drug Delivery

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