G613 SCIN: surveillance of congenital ichthyosis in neonates in UK and Ireland: a BPSU interim report

Abstract

Technical validation of an EV-D68 PCR.. Determine whether a one-step PCR is sufficient or whether a two-step PCR is required for detection of the virus in respiratory tract samples.. Determine whether any samples that tested HRV positive in our hospital between July 2018-January 2019 were in fact EV-D68 positive. Methods and Results. Primers and probes selected according to those already validated in Poelmana et al and tested in 1 step PCR with negative and positive control: successful.. Sensitivity tested against NGS sequenced EV-D68 positive samples from our own laboratory, 5 from another teaching hospital and 2 QCMD samples: 1 and 2 step PCR sensitive, including 2-step PCR with internal control.. Specificity successfully tested against many non-EV-68 samples positive for other viruses and bacteria.. Comparison of Cq values of 1 step PCR versus 2 step PCR with internal control. Cq values 2-step PCR < Cq values 1step PCR in all dilutions from 1:4-1:4000. 1-step did not detect virus >1:400 dilution.. Epidemiological study retrospectively testing all 104 HRV positive respiratory samples and DNA isolates from last week July 2018 to second week January 2019 with 2-step PCR. There were 8 positive cases, clustered in November and December. 49 35.24 35.3 Nasal aspirate 1 25.53