G894T endothelial nitric oxide synthase polymorphism and ischemic stroke in Morocco

Diakité, Bréhima; Hamzi, Khalil; Yahyaoui, M.; Alaoui, Moulay; Habbal, Rachida; Nadifi, Sellama

doi:10.1016/j.mgene.2014.04.003

Cited by 19 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, this SNP was found to be associated with a higher risk towards stroke in other Asian populations such as in Indian population (Kumar et al 2016) and Taiwanese population (Shyu et al 2017), indicating that the association of this SNP with stroke risk is ethnic-dependent as the populations in Malaysia and Singapore consist of After stratification according to gender, we found that Malaysian males with the eNOS rs1799983-GT genotype had a significant increase risk towards stroke. However, studies based on other populations such as the Morocco population (Diakite et al 2014), Tunisian population (Saidi et al 2010) and German population (Berger et al 2007) had found that the association of this polymorphism with stroke was independent according to gender. The function of eNOS rs1799983 SNP has been well-studied but there are no studies reporting this SNP plays a different role in males and females.…”

Section: Discussionmentioning

confidence: 93%

A Case-Control Study and Meta-Analysis of the Association of eNOS rs1799983 SNP with Stroke Risk

Lee¹

2019

View full text Add to dashboard Cite

The endothelial nitric oxide synthase (eNOS) rs1799983 polymorphism is known to increase the risk towards stroke, but data is under-reported in Malaysian population. Therefore, this study sought to investigate this association in a Malaysian population and in a comprehensive meta-analysis. Genotyping of the eNOS rs1799983 polymorphism was performed for 241 Malaysians using a hydrolysis probe. Odd ratio with 95% confidence interval was calculated. Meta-analysis was conducted using the Comprehensive Meta-Analysis software ver. 2.2.064. A p-value less than 0.05 was considered statistically significant. Overall, our results showed that the presence of eNOS rs1799983-T allele increases the risk towards stroke, particularly in males, fast-food goers and Malaysian Chinese. The meta-analysis showed that the rs1799983 polymorphism is significantly associated with an increase ischemic stroke risk in the recessive and allelic models. After stratified with population, these associations remain significant in the Asian population but not in the Caucasian population. In summary, this study establishes a significant relationship between the eNOS rs1799983 polymorphism with gender, lifestyle and ethnicity differences towards stroke risk in the Malaysian population. In addition, our meta-analysis suggests that the eNOS rs1799983 polymorphism is associated with an increase risk of ischemic stroke.

show abstract

Section: Discussionmentioning

confidence: 93%

A Case-Control Study and Meta-Analysis of the Association of eNOS rs1799983 SNP with Stroke Risk

Lee¹

2019

View full text Add to dashboard Cite

show abstract

“…A significant relationship between the T allele and TT genotype of the G894T polymorphism and ischemic stroke risk was found in Asians and north Indians, but not in Caucasians (Table 4) (3,6,10,17,18). In Morocco, Diakite et al reported a significant relationship between the G894T eNOS polymorphism and ischemic stroke in recessive, dominant, and codominant models ( Table 5) [16].…”

Section: G894t Polymorphismmentioning

confidence: 98%

Investigation of the relationship between ischemic stroke and endothelial nitric oxide synthase gene polymorphisms [G894T, intron 4 VNTR and T786C]

Anlıaçık¹

2019

Turk J Med Sci

View full text Add to dashboard Cite

According to the WHO, stroke is a clinical syndrome caused by vascular lesion. It is characterized by rapidly occurring clinical symptoms and/or signs of focal or generalized cerebral dysfunction lasting more than 24 h or resulting in death [1]. Despite improvements in the treatment of acute stroke, strokes continue to represent the third-leading cause of death in many countries. Therefore, identification and prevention of stroke risk factors are gaining increasing importance. Approximately 70% of stroke risk is attributed to known etiological risk factors, and genetic factors may account for a significant proportion among the remaining unidentified causes [2]. Polymorphisms affecting various genes, such as angiotensin converting enzyme (ACE), thrombinactivatable fibrinolysis inhibitor (TAFI), and nitric oxide synthase (NOS) genes, as suggested by recent advances in genetic research techniques are associated with the incidence of ischemic stroke (3-9). Nitric oxide (NO), an enzyme that regulates the endothelial microenvironment by reducing intracellular calcium levels, inhibits platelet aggregation and vascular smooth muscle cell proliferation, and reduces leukocyte adhesion [4,10]. The enzyme NOS catalyzes a reaction whereby L-arginine is synthesized from the N-terminus of guanidine. There are two forms of NOS: constitutive and inducible. Different genes synthesize different forms of NOS [NOS1 (neuronal NOS), NOS2, and NOS3 (endothelial NOS)] enzymes [11]. The NOS also inhibits the oxidation of low-density lipoprotein, thereby regulating the endothelial microenvironment [4,10]. Thus, any genetic problem disrupting the amount and structure of NO will cause an inclination towards clotting. In some populations, although a significant relationship was found between the risk of ischemic stroke Background/aim: We aimed to investigate the associations between endothelial nitric oxide synthase (eNOS) gene polymorphisms [G894T (rs1799983)], intron 4 (27-bpTR) variable number tandem repeat (VNTR) and T786C (rs2070744), and ischemic stroke in the Anatolian population. Materials and methods: This case-control study included 112 patients with "stroke of undetermined etiology" and 160 controls. Realtime polymerase chain reaction (RT-PCR) analysis was used to analyze these polymorphisms. Between-group frequencies of alleles and genotypes were compared using binary logistic regression analysis. Results: No significant difference was observed between the two groups in terms of the genotype and allele distributions of the eNOS G894T (rs1799983) polymorphism (P > 0.05). The a alleles and the 4b/a and 4a/a genotypes of the intron 4 (27-bpTR) VNTR polymorphism had significantly higher frequencies in the patient group than in the control group (OR: 2.715, P < 0.001; OR: 3.396, P < 0.001; OR: 10.631, P = 0.016, respectively). On the contrary, the TC genotype and C alleles of the T786C (rs2070744) polymorphism had a significantly lower frequency in the patient group than in the control group (OR: 0.244, P < 0.001, OR: 0.605, P = 0.006,...

show abstract

“…Regarding its critical role in vascular system and blood flow regulation, NO-the main product of endothelial NOS enzyme-has been implicated in the occurrence of numerous pathologies, such as ischemic stroke (Diakite et al, 2014), HTA (Tang et al, 2008), renal disease (Aldámiz-Echevarría and Andrade, 2012), and cardiovascular diseases (Charalambos et al, 2006). According to the literature, studies exploring the association of G894T eNOS polymorphism with MI predisposition still remain inconclusive and results are contradictory (Luo et al, 2014).…”

Section: Enosmentioning

confidence: 99%

“…NO production-known for its role in regulation of blood pressure variability-is decreased and endothelial dysfunction occurs more often, leading to myocardial infarction development (Zhang et al, 2012;Song et al, 2013;Gong et al, 2012;Forstermann and Munzel, 2006;Franco et al, 2007;Diakite et al, 2014;Tang et al, 2008;Aldámiz-Echevarría and Andrade, 2012;Charalambos et al, 2006;Luo et al, 2014;Ben Ali et al, 2015;Casas et al, 2004;Veldman et al, 2002;Rankinen et al, 2000).…”

Section: Enosmentioning

confidence: 99%

Association of G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms with susceptibility to myocardial infarction in Morocco

Idrissi¹,

Hmimech²,

Diakité³

et al. 2017

Archives of Cardiovascular Diseases Supplements

View full text Add to dashboard Cite

Background: Myocardial infarction (MI) is a common multifactorial disease. Numerous studies have found that genetic plays an essential role in MI occurrence. The main objective of our case-control study is to explore the association of G894T eNOS (rs1799983), 4G/5G PAI (rs1799889) and T1131C APOA5 (rs662799) polymorphisms with MI susceptibility in the Moroccan population. Methods and results: 118 MI patients were recruited vs 184 healthy controls. DNA samples were genotyped by PCR-RFLP method using MboI, BslI and MseI restriction enzymes respectively for the G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms. Our results show that the G894T eNOS was significantly associated with increased risk of MI under the three genetic transmission models (dominant: OR = 1.64, 95% CI = 1.05-2.58, P = 0.003; recessive: OR = 2.15, 95% CI = 0.74-6.16, P = 0.03; additive: OR = 1.54, 95% CI = 1.06-2.23, P = 0.001). The T1131C APOA5 polymorphism was associated to MI risk in recessive and additive models (OR = 1.53, 95% CI = 0.72-3.2, P = 0.04 and OR = 1.78, 95% CI = 1.26-2.51, P = 0.03 respectively). For the 4G/5G PAI variant, even the cases and controls groups were not in Hardy-Weinberg Equilibrium (HWE), the dominant and additive models show a statistically significant association with MI risk (OR = 7.96,, P = 0.01 and OR = 1.96, 95% CI = 1.4-2.72, P = 0.03 respectively). Conclusion: Our results suggest that G894T eNOS and T1131C APOA5 polymorphisms may be considered as genetic markers of MI among the Moroccan population. Further studies including larger sample sizes and exploring more genetic associations are needed to confirm our results and to better understand the susceptibility to MI.

show abstract

G894T endothelial nitric oxide synthase polymorphism and ischemic stroke in Morocco

Cited by 19 publications

References 33 publications

A Case-Control Study and Meta-Analysis of the Association of eNOS rs1799983 SNP with Stroke Risk

A Case-Control Study and Meta-Analysis of the Association of eNOS rs1799983 SNP with Stroke Risk

Investigation of the relationship between ischemic stroke and endothelial nitric oxide synthase gene polymorphisms [G894T, intron 4 VNTR and T786C]

Association of G894T eNOS, 4G/5G PAI and T1131C APOA5 polymorphisms with susceptibility to myocardial infarction in Morocco

Contact Info

Product

Resources

About