GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells

Du, Yiqin; Du, Zhao; Zheng, Huakun; Wang, Dan; Li, Shifeng; Yan, Yonggang; Li, Yiping

doi:10.2478/s11658-013-0081-4

Cited by 17 publications

(20 citation statements)

References 37 publications

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“…However, when HS significantly reduced the expression of GABA receptor mRNA, the GABA receptor functioned as a negative regulator of HS response, leading to a weakened inhibition. Previous studies have shown that treatment of spermatogonial stem cells with GABA increases the expression of GABA receptor, but inhibits cell proliferation, while treating oocytes with GABA increases the expression of GABA receptor, but reduces estrogen production (Biggs et al, 2013;Du et al, 2013). The animal's body can regulate sexual development and maturation by secreting hormones of the HPG axis, which is the center controlling the development and function of reproductive system in animals.…”

Section: Discussionmentioning

confidence: 99%

Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Xie

et al. 2016

Rev. Bras. Cienc. Avic.

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We investigated the effect of heat stress (HS) on the expression of the GABA receptor in the hypothalamic-pituitary-gonadal (HPG) axis of Wenchang chickens. Real-time quantitative RT-PCR (qRT-PCR) was used to quantify the GABA receptor mRNA levels along the HPG axis of chickens under HS (40±0.5 °C) for 1-6 weeks. Our results showed that the expression of GABA A and GABA B receptor at the mRNAs levels in the tissues of HPG axis exhibited fluctuation and variability. After HS, the mRNA level of GABA A receptor was significantly reduced in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but significantly increased in the pituitary of 1-, 4-, and 5-weekold chickens. The GABA B receptor mRNA level significantly declined in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but was significantly upregulated in the pituitary and testis of 1-and 2-week-old chickens. At other time points, the expressions of GABA A receptor and GABA B receptor showed no significant differences compared with control group. These results indicated that the levels of GABA A receptor and GABA B receptor mRNAs varied in different tissues of the HPG axis in chickens of different ages, displaying temporal and spatial variations. GABA receptor behaved as a positively-regulated gene by HS, i.e., its mRNA was increased by HS; similarly, it was a negativelyregulated gene by HS, when its expression was reduced by HS.

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Section: Discussionmentioning

confidence: 99%

Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Xie

et al. 2016

Rev. Bras. Cienc. Avic.

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“…, 2013 ), embryonic stem cells ( Ng et al. , 2010 ; Du et al. , 2013 ), myoblasts (in which hyperpolarization driven by the Kir2.1 channel plays a key role; Hinard et al.…”

Section: New Control Knobs: Resting Potential Determines Single-cell mentioning

confidence: 99%

Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo

Levin

2014

MBoC

308

325

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In addition to biochemical gradients and transcriptional networks, cell behavior is regulated by endogenous bioelectrical cues originating in the activity of ion channels and pumps, operating in a wide variety of cell types. Instructive signals mediated by changes in resting potential control proliferation, differentiation, cell shape, and apoptosis of stem, progenitor, and somatic cells. Of importance, however, cells are regulated not only by their own Vmem but also by the Vmem of their neighbors, forming networks via electrical synapses known as gap junctions. Spatiotemporal changes in Vmem distribution among nonneural somatic tissues regulate pattern formation and serve as signals that trigger limb regeneration, induce eye formation, set polarity of whole-body anatomical axes, and orchestrate craniofacial patterning. New tools for tracking and functionally altering Vmem gradients in vivo have identified novel roles for bioelectrical signaling and revealed the molecular pathways by which Vmem changes are transduced into cascades of downstream gene expression. Because channels and gap junctions are gated posttranslationally, bioelectrical networks have their own characteristic dynamics that do not reduce to molecular profiling of channel expression (although they couple functionally to transcriptional networks). The recent data provide an exciting opportunity to crack the bioelectric code, and learn to program cellular activity at the level of organs, not only cell types. The understanding of how patterning information is encoded in bioelectrical networks, which may require concepts from computational neuroscience, will have transformative implications for embryogenesis, regeneration, cancer, and synthetic bioengineering.

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“…A particularly thorough combination of biochemistry, transgenic mouse technology, and electric field perturbation dissected the mechanisms of electrotaxis showing that mammalian wound healing requires cells to sense endogenous fields (generated by trans‐epithelial potential) by a PTEN ‐ and PI(3)K‐γ‐dependent pathway. Cell differentiation is also controlled by changes in V mem , as has been shown in human mesenchymal stem cells, embryonic stem cells, myoblasts (in which hyperpolarization driven by the Kir2.1 channel plays a crucial role), the specification of neurotransmitter types, and the control of precursor differentiation in the developing nervous system and heart. Tissue engineers have also begun to take advantage of this pathway using applied electric field stimulation .…”

Section: Bioelectrical Determinants Of Individual Cell Behaviormentioning

confidence: 90%

Reprogramming cells and tissue patterning via bioelectrical pathways: molecular mechanisms and biomedical opportunities

Levin

2013

WIREs Mechanisms of Disease

105

179

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Transformative impact in regenerative medicine requires more than the reprogramming of individual cells: advances in repair strategies for birth defects or injuries, tumor normalization, and the construction of bioengineered organs and tissues all require the ability to control large-scale anatomical shape. Much recent work has focused on the transcriptional and biochemical regulation of cell behaviour and morphogenesis. However, exciting new data reveal that bioelectrical properties of cells and their microenvironment exert a profound influence on cell differentiation, proliferation, and migration. Ion channels and pumps expressed in all cells, not just excitable nerve and muscle, establish resting potentials that vary across tissues and change with significant developmental events. Most importantly, the spatio-temporal gradients of these endogenous transmembrane voltage potentials (Vmem) serve as instructive patterning cues for large-scale anatomy, providing organ identity, positional information, and prepattern template cues for morphogenesis. New genetic and pharmacological techniques for molecular modulation of bioelectric gradients in vivo have revealed the ability to initiate complex organogenesis, change tissue identity, and trigger regeneration of whole vertebrate appendages. A large segment of the spatial information processing that orchestrates individual cells’ programs towards the anatomical needs of the host organism is electrical; this blurs the line between memory and decision-making in neural networks and morphogenesis in non-neural tissues. Advances in cracking this bioelectric code will enable the rational reprogramming of shape in whole tissues and organs, revolutionizing regenerative medicine, developmental biology, and synthetic bioengineering.

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GABA exists as a negative regulator of cell proliferation in spermaogonial stem cells

Cited by 17 publications

References 37 publications

Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Effect of Heat Stress on the Expression of GABA Receptor mRNA in the HPG Axis of Wenchang Chickens

Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo

Reprogramming cells and tissue patterning via bioelectrical pathways: molecular mechanisms and biomedical opportunities

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